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Human MMP-1(Matrix Metalloproteinase 1) ELISA Kit

Citations(19)Uniprot : P03956

Cat.No.:E-EL-H6073
Reactivity: Human

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	31.25-2000 pg/mL
Sensitivity	18.75 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Human MMP-1 in samples. No significant cross-reactivity or interference between Human MMP-1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human MMP-1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MMP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MMP-1. You can calculate the concentration of Human MMP-1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human MMP-1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human MMP-1 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	85.33	246.42	880.11	82.77	242.81	921.97
Standard deviation	4.95	11.58	36.08	4.59	11.07	47.02
CV (%)	5.80	4.70	4.10	5.55	4.56	5.10

Recovery

The recovery of Human MMP-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	82-98	90
EDTA plasma (n=8)	89-102	95
Cell culture media (n=8)	98-108	105

Linearity

Samples were spiked with high concentrations of Human MMP-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P03956
Synonyms	MMP1, CLG, CLGN, Interstitial Collagenase, Fibroblast Collagenase
Research Area	Cancer, Cell Biology, Cardiovascular, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Nature Communications (2021) IF: 14.919
Geometrically encoded SERS nanobarcodes for the logical detection of nasopharyngeal carcinoma-related progression biomarkers

DOI: 10.1038/s41467-021-23789-3

PMID: 34078895
Sample: plasma
Antioxidants (2022) IF: 7.675
Anti-Aging Effect and Mechanism of Proanthocyanidins Extracted from Sea buckthorn on Hydrogen Peroxide-Induced Aging Human Skin Fibroblasts

DOI: 10.3390/antiox11101900

PMID: 36290623
Sample: HSFs
Cells (2021) IF: 6.6
The Stiffness of Cardiac Fibroblast Substrates Exerts a Regulatory Influence on Collagen Metabolism via α2β1 Integrin, FAK and Src Kinases

DOI: 10.3390/cells10123506

Sample: cardiac fibroblast
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY (2022) IF: 6.814
Potential anti-skin aging effect of a peptide AYAPE isolated from Isochrysis zhanjiangensis on UVB-induced HaCaT cells and H2O2-induced BJ cells

DOI: 10.1016/j.jphotobiol.2022.112481

Sample: HaCaT cell
Oncotarget (2016) IF: 5.008
MMP-1 promoter polymorphism is associated with risk of radiation-induced lung injury in lung cancer patients treated with radiotherapy

DOI: 10.18632/oncotarget.12164

Sample: Plasma
Marine Drugs (2021) IF: 5.118
Heptapeptide Isolated from Isochrysis zhanjiangensis Exhibited Anti-Photoaging Potential via MAPK/AP-1/MMP Pathway and Anti-Apoptosis in UVB-Irradiated HaCaT Cells

DOI: 10.3390/md19110626

PMID: 34822497
Sample: HaCaT cells
CHEMICO-BIOLOGICAL INTERACTIONS (2021) IF: 5.194
A novel glyceroglycolipid from brown algae Ishige okamurae improve photoaging and counteract inflammation in UVB-induced HaCaT cells

DOI: 10.1016/j.cbi.2021.109737

PMID: 34740599
Sample: HaCaT cells
INDUSTRIAL CROPS AND PRODUCTS (2022) IF: 5.645
Preparation and evaluation of photoprotective kenaf seed oil nanocarriers-based cream of tocotrienol-rich fraction

DOI: 10.1016/j.indcrop.2022.115164

Sample: Normal human dermal fibroblasts (NHDF)
Frontiers in Pharmacology (2022) IF: 5.988
Aconiti lateralis radix praeparata total alkaloids exert anti-RA effects by regulating NF-κB and JAK/STAT signaling pathways and promoting apoptosis.

DOI: 10.3389/fphar.2022.980229

Sample: MH7A cell
Spine Journal (2022) IF: 4.166
Extracellular vesicle and soluble fractions of adipose tissue-derived mesenchymal stem cells secretome induce inflammatory cytokines modulation in an in vitro model of discogenic pain

DOI: 10.1016/j.spinee.2022.01.012

PMID: 35121152
Sample: human adipose-derived mesenchymal stem cells (HAdM
JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36
Anti-proliferation and anti-inflammation effects of corilagin in rheumatoid arthritis by downregulating NF-κB and MAPK signaling pathways

DOI: 10.1016/j.jep.2021.114791

PMID: 34737112
Sample: MH7A cell
MOLECULES (2021) IF: 4.412
Insight into Seasonal Change of Phytochemicals, Antioxidant, and Anti-Aging Activities of Root Bark of Paeonia suffruticosa (Cortex Moutan) Combined with Multivariate Statistical Analysis

DOI: 10.3390/molecules26206102

PMID: 34684685
Sample: HDF cell
Bioengineered (2022) IF: 3.269
The protective effects of Olmesartan against interleukin-29 (IL-29)-induced type 2 collagen degradation in human chondrocytes

DOI: 10.1080/21655979.2021.1997090

PMID: 35012432
Sample: T/C-28a2 cell
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH (2022) IF: 3.657
Can the Prognosis of COVID-19 Disease Be Determined by Fecal Markers and Cytokines?

DOI: 10.1089/jir.2022.0098

PMID: 36179036
Sample: fecal
Journal of Functional Foods (2020) IF: 3.701
Trehalose against UVB-induced skin photoaging by suppressing MMP expression and enhancing procollagen I synthesis in HaCaT cells

DOI: 10.1016/j.jff.2020.104198

Sample: HaCaT cell
Experimental and Therapeutic Medicine (2021) IF: 2.447
Vicenin 3 ameliorates ECM degradation by regulating the MAPK pathway in SW1353 chondrocytes

DOI: 10.3892/etm.2021.10896

Sample: SW1353 cell
Genetic Testing and Molecular Biomarkers (2019) IF: 1.121
Association of PAR-2 Gene Polymorphisms with the Inflammatory Response and Susceptibility to Knee Osteoarthritis in the Chinese Han Population

DOI: 10.1089/gtmb.2018.0219

PMID: 30688539
Sample: Synovia
Iranian Journal of Basic Medical Sciences (2018) IF: 1.514
Effects of insulin-like growth factor-induced Wharton jelly mesenchymal stem cells toward chondrogenesis in an osteoarthritis model

DOI: 10.22038/IJBMS.2018.28205.6840

Sample: Serum
EXPERIMENTAL AND CLINICAL ENDOCRINOLOGY & DIABETES (2017) IF: 1.685
Expression and Significance of MMPs in Synovial Fluid, Serum and PBMC Culture Supernatant Stimulated by LPS in Osteoarthritis Patients With or Without Diabetes

DOI: 10.1055/s-0043-122223

PMID: 29270923
Sample: Serum,Synovia

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