Current Position:Home>>CLIA Kits>> Human PTGES (Prostaglandin E Synthase, Microsomal) CLIA Kit
Synonyms:MGST-IV, MGST1-L1, MGST1L1, MPGES, PIG12, PP102, PP1294, TP53I12, mPGES-1
Detection Range: 31.25~2000 pg/mL
Sensitivity: 18.75 pg/mL
This CLIA kit applies to the in vitro quantitative determination of Human PTGES concentrations in serum, plasma and other biological fluids.
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human PTGES. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PTGES and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PTGES, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human PTGES. You can calculate the concentration of Human PTGES in the samples by comparing the RLU value of the samples to the standard curve.
|Detection range||31.25-2000 pg/mL|
|Sample type||Serum, plasma and other biological fluids|
|Repeatability||CV < 15%|
This kit recognizes Human PTGES in samples. No significant cross-reactivity or interference between Human PTGES and analogues was observed.
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human PTGES were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human PTGES were tested on 3 different plates, 20 replicates in each plate.
|Intra-assay Precision||Inter-assay Precision|
|C V (%)||12.80||11.27||6.04||10.18||7.83||8.17|
The recovery of Human PTGES spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
|Sample Type||Range (%)||Average Recovery (%)|
|EDTA plasma (n=5)||84-98||91|
|Cell culture media (n=5)||96-112||103|
Samples were spiked with high concentrations of Human PTGES and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
|Serum (n=5)||EDTA plasma (n=5)||Cell culture media (n=5)|
|Micro CLIA Plate(Dismountable)||8 wells ×12 strips||-20℃, 6 months|
|Reference Standard||2 vials|
|Concentrated Biotinylated Detection Ab (100×)||1 vial, 120 μL|
|Concentrated HRP Conjugate (100×)||1 vial, 120 μL||-20℃(shading light), 6 months|
|Reference Standard & Sample Diluent||1 vial, 20 mL||4℃, 6 months|
|Biotinylated Detection Ab Diluent||1 vial, 14 mL|
|HRP Conjugate Diluent||1 vial, 14 mL|
|Concentrated Wash Buffer (25×)||1 vial, 30 mL|
|Substrate Reagent A||1 vial, 5 mL||4℃ (shading light)|
|Substrate Reagent B||1 vial, 5 mL||4℃ (shading light)|
|Plate Sealer||5 pieces|
|Product Description||1 copy|
|Certificate of Analysis||1 copy|
The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).
1.Add 100 μL of standard or sample to each well. Incubate for 90 min at 37℃.
2.Remove the liquid.
3.Add 100 μL Biotinylated Detection Ab. Incubate 1 hour at 37℃. Aspirate and wash 3 times.
4.Add 100 μL HRP Conjugate. Incubate 30 min at 37℃. Aspirate and wash 5 times.
5.Add 100 μL Substrate Mixture Solution. Incubate for 5 min at 37℃.