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Human SFRP2 (Secreted Frizzled Related Protein 2) CLIA Kit

Cat:E-CL-H0858
Manual MSDS

Price: $ 682

Price: $ 546

Price: $ 180

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Price: Inquire

Size:
96T 48T 24T 96T*10 96T*5
Quantity:
  • Reactivity: Human
  • Detection Range: 0.31-20 ng/mL
  • Sensitivity: 0.19 ng/mL
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Product Details
Test Principle This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SFRP2. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SFRP2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SFRP2, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human SFRP2. You can calculate the concentration of Human SFRP2 in the samples by comparing the RLU value of the samples to the standard curve.
Assay Type Sandwich-ELISA
size 96T / 48T / 24T / 96T*10 / 96T*5
Assay Time 3.5h
Detection Method Chemiluminescence
Detection Range 0.31-20 ng/mL
Sensitivity 0.19 ng/mL
Sample Volume 100μL
Sample Type serum, plasma and other biological fluids
Specificity This kit recognizes Human SFRP2 in samples.No significant cross-reactivity or interference between Human SFRP2 and analogues was observed
Precision Both intra-CV and inter-CV are < 10%.
Recovery 80%-120%
Introduction This ELISA kit applies to the in vitro quantitative determination of Human SFRP2 concentrations in serum, plasma and other biological fluids.
Applications CLIA
Storage 2-8℃/-20℃
Database SwissProt:  Q96HF1
Research Area Cancer Cardiovascular Stem Cells