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Human TGF-β1(Transforming Growth Factor Beta 1) ELISA Kit

Citations(40)Uniprot : P01137

  • Cat.No.:E-EL-H0110

  • Reactivity: Human
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  • 96T
  • 24T
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T
Assay time 3.5h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume Serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human TGF-β1 in samples. No significant cross-reactivity or interference between Human TGF-β1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human TGF-β1 concentrations in Serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TGF-β1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TGF-β1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TGF-β1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human TGF-β1. You can calculate the concentration of Human TGF-β1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human TGF-β1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human TGF-β1 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 106.20 233.50 976.30 109.60 234.10 999.00
Standard deviation 7.20 11.90 51.70 5.60 12.40 43.00
CV (%) 6.78 5.10 5.30 5.11 5.30 4.30

Recovery

The recovery of Human TGF-β1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-105 100
EDTA plasma (n=8) 90-104 96
Cell culture media (n=8) 87-100 92

Target Information

Database LinksSwissProt:   P01137
SynonymsTGFB1, CED, DPD1, LAP, TGFB, TGFbeta, transforming growth factor beta 1
Research AreaCancer,Cardiovascular,Signaling transduction,Stem cells

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Theranostics (2019) IF: 8.0630
    Temporal inhibition of mouse mammary gland cancer metastasis by CORM-A1 and DETA/NO combination therapy

    DOI: 10.7150/thno.31461
    Sample: platelet poor plasma (PPP)
  2. CELL PROLIFERATION (2022) IF: 8.7550
    Tetrahedral framework nucleic acids promote diabetic wound healing via the Wnt signalling pathway

    DOI: 10.1111/cpr.13316

    PMID: 35869570

    		 Sample: HDFs
  3. Biomolecules (2022) IF: 6.064
    Serum Levels of Transforming Growth Factor Beta 1 in Systemic Lupus Erythematosus Patients

    DOI: 10.3390/biom13010073
    Sample: serum
  4. Food & Function (2022) IF: 6.317
    Whole grain germinated brown rice regulates intestinal immune homeostasis and gastrointestinal hormones in type 2 diabetic patients—a randomized control trial

    DOI: 10.1039/D2FO00477A
    Sample: serum
  5. Oxidative Medicine and Cellular Longevity (2022) IF: 6.543
    DNA Methyltransferase 3b Accelerates the Process of Atherosclerosis

    DOI: 10.1155/2022/5249367

    PMID: 35422896

    		 Sample: Tregs
  6. JOURNAL OF PERIODONTOLOGY (2021) IF: 6.993
    The effects of a novel non-invasive application of platelet-rich fibrin on periodontal clinical parameters and gingival crevicular fluid transforming growth factor-β and collagen-1 levels: A randomized, controlled, clinical study

    DOI: 10.1002/JPER.20-0713

    PMID: 33382101

    		 Sample: gingival crevicular fluid (GCF)
  7. Aging-US (2019) IF: 5.515
    Laminin α4 overexpression in the anterior lens capsule may contribute to the senescence of human lens epithelial cells in age-related cataract

    DOI: 10.18632/aging.101943
    Sample: anterior lens capsules (ALCs),HLE B-3 cells
  8. JOURNAL OF CELLULAR PHYSIOLOGY (2020) IF: 5.546
    Cytokine physiognomies of MSCs from varied sources confirm the regenerative commitment post-coculture with activated neutrophils

    DOI: 10.1002/jcp.29713

    PMID: 32385929

    		 Sample: Cell culture supernatant
  9. INDUSTRIAL CROPS AND PRODUCTS (2022) IF: 5.645
    Preparation and evaluation of photoprotective kenaf seed oil nanocarriers-based cream of tocotrienol-rich fraction

    DOI: 10.1016/j.indcrop.2022.115164
    Sample: Normal human dermal fibroblasts (NHDF)
  10. Nutrients (2021) IF: 5.719
    Tocotrienol-Rich Vitamin E (Tocovid) Improved Nerve Conduction Velocity in Type 2 Diabetes Mellitus Patients in a Phase II Double-Blind, Randomized Controlled Clinical Trial

    DOI: 10.3390/nu13113770

    PMID: 34836025

    		 Sample: serum
  11. CELL AND TISSUE RESEARCH (2022) IF: 4.051
    Sestrin2 attenuates renal damage by regulating Hippo pathway in diabetic nephropathy

    DOI: 10.1007/s00441-022-03668-z

    PMID: 35821438

    		 Sample: HMCs
  12. JOURNAL OF BIOLOGICAL CHEMISTRY (2019) IF: 4.106
    KGF-1 accelerates wound contraction through the TGF-β1/Smad signaling pathway in a double-paracrine manner

    DOI: 10.1074/jbc.RA118.006189
    Sample: Cell culture medium
  13. ENVIRONMENTAL TOXICOLOGY (2022) IF: 4.119
    LncRNA MEG3 ameliorates NiO nanoparticles-induced pulmonary inflammatory damage via suppressing the p38 mitogen activated protein kinases pathway

    DOI: 10.1002/tox.23464

    PMID: 35006638

    		 Sample: A549 cell
  14. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE (2020) IF: 4.352
    S-adenosyl-L-methionine (SAMe) halts the autoimmune response in patients with primary biliary cholangitis (PBC) via antioxidant and S-glutathionylation processes in cholangiocytes

    DOI: 10.1016/j.bbadis.2020.165895

    PMID: 32681864

    		 Sample: Serum
  15. PLoS Neglected Tropical Diseases (2021) IF: 4.411
    Anaphylatoxins orchestrate Th17 response via interactions between CD16+ monocytes and pleural mesothelial cells in tuberculous pleural effusion

    DOI: 10.1371/journal.pntd.0009508

    PMID: 34237073

    		 Sample: pleural fluid
  16. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2019) IF: 4.658
    The role and mechanism of transforming growth factor beta 3 in human myocardial infarction‐induced myocardial fibrosis

    DOI: 10.1111/jcmm.14313
    Sample: Serum
  17. Aging-US (2020) IF: 4.831
    Cyclooxygenase-2/sclerostin mediates TGF-β1-induced calcification in vascular smooth muscle cells and rats undergoing renal failure

    DOI: 10.18632/aging.103827

    PMID: 33159018

    		 Sample: Serum
  18. INTERNATIONAL IMMUNOPHARMACOLOGY (2018) IF: 3.118
    Novel anti-inflammatory target of geniposide: Inhibiting Itgβ1/Ras-Erk1/2 signal pathway via the miRNA-124a in rheumatoid arthritis synovial fibroblasts

    DOI: 10.1016/j.intimp.2018.09.049
    Sample: MH7A cells
  19. Diabetes Metabolic Syndrome and Obesity-Targets and Therapy (2021) IF: 3.168
    Inflammatory Biomarkers Levels in T2DM Emirati Patients with Diabetic Neuropathy

    DOI: 10.2147/DMSO.S319863

    PMID: 34345175

    		 Sample: serum
  20. Clinical & Translational Oncology (2022) IF: 3.34
    Cancer cell-derived exosomal LINC00313 induces M2 macrophage differentiation in non-small cell lung cancer

    DOI: 10.1007/s12094-022-02907-7

    PMID: 35980503

    		 Sample: THP-1 cell,H1299 cell
  21. RSC Advances (2019) IF: 3.361
    miR-let-7d attenuates EMT by targeting HMGA2 in silica-induced pulmonary fibrosis

    DOI: 10.1039/C9RA01031A
    Sample: macrophages
  22. Frontiers in Physiology (2018) IF: 3.394
    Participation of Arachidonic Acid Metabolism in the Aortic Aneurysm Formation in Patients with Marfan Syndrome

    DOI: 10.3389/fphys.2018.00077
    Sample: aortic tissue
  23. ORAL DISEASES (2022) IF: 3.511
    Influence of Periodontitis and Diabetes on Structure and Cytokine content of Platelet-Rich Fibrin

    DOI: 10.1111/odi.14275

    PMID: 35699366

    		 Sample: plasma
  24. CELL BIOLOGY INTERNATIONAL (2022) IF: 3.612
    The positive feedback loop of furin and TGFβ1 enhances the immune responses of Tregs to hepatocellular carcinoma cells and hepatitis B virus in vitro

    DOI: 10.1002/cbin.11806

    PMID: 35349767

    		 Sample: HepG2 cell
  25. Biology-Basel (2020) IF: 3.796
    Induction of CD4+CD25+ Regulatory T Cells from In Vitro Grown Human Mononuclear Cells by Sparteine Sulfate and Harpagoside

    DOI: 10.3390/biology9080211
    Sample: Cell culture supernatant
  26. JOURNAL OF ALZHEIMERS DISEASE (2020) IF: 3.909
    Cognitive Impairment, P300, and Transforming Growth Factor β1 in Different Forms of Dementia

    DOI: 10.3233/JAD-200885
    Sample: serum
  27. Revista da Sociedade Brasileira de Medicina Tropical (2022) IF: 2.141
    Role of transforming growth factor-beta 1 and connective tissue growth factor levels in coronavirus disease-2019-related lung Injury: a prospective, observational, cohort study

    DOI: 10.1590/0037-8682-0615-2021

    PMID: 35894403

    		 Sample: blood
  28. BMC Cardiovascular Disorders (2021) IF: 2.298
    The potential regulatory role of hsa_circ_0004104 in the persistency of atrial fibrillation by promoting cardiac fibrosis via TGF-β pathway

    DOI: 10.1186/s12872-021-01847-4

    PMID: 33421993

    		 Sample: Plasma
  29. MOLECULAR BIOLOGY REPORTS (2021) IF: 2.316
    SMAD2, SMAD3 and TGF-β GENE expressions in women suffering from urge urinary incontinence and pelvic organ prolapse

    DOI: 10.1007/s11033-021-06220-4

    PMID: 33599951

    		 Sample: Tissue（uterosacral ligament）
  30. INTERNATIONAL JOURNAL OF CLINICAL PRACTICE (2021) IF: 2.503
    Serum periostin levels in COVID-19: Is it useful as a new biomarker?

    DOI: 10.1111/ijcp.14728
    Sample: serum
  31. INVESTIGATIONAL NEW DRUGS (2019) IF: 2.663
    The rhenium(I)-diselenoether anticancer drug targets ROS, TGF-β1, VEGF-A, and IGF-1 in an in vitro experimental model of triple-negative breast cancers

    DOI: 10.1007/s10637-019-00727-1

    PMID: 30632005

    		 Sample: Cell culture medium
  32. Applied Sciences-Basel (2021) IF: 2.679
    Biochemical Mapping of the Inflamed Human Dental Pulp

    DOI: 10.3390/app112110395
    Sample: dental pulp lysates
  33. Applied Sciences-Basel (2022) IF: 2.679
    Assessment of Mineralization, Oxidative Stress, and Inflammation Mechanisms in the Pulp of Primary Teeth

    DOI: 10.3390/app12031554
    Sample: Dental pulp tissue
  34. BIOMEDICAL AND ENVIRONMENTAL SCIENCES (2022) IF: 2.831
    Periodontal Ligament Stem Cells Reverse the High Glucose Level-Induced Inflammatory State of Macrophages

    DOI: 10.3967/bes2022.089

    PMID: 35945185

    		 Sample: THP-1 cell
  35. HUMAN & EXPERIMENTAL TOXICOLOGY (2019) IF: 2.903
    Lipopolysaccharide induces epithelial–mesenchymal transition of alveolar epithelial cells cocultured with macrophages possibly via the JAK2/STAT3 signaling pathway:

    DOI: 10.1177/0960327119881678
    Sample: Cell culture supernatant
  36. TUBERCULOSIS (2022) IF: 2.973
    Inflammation-mediated changes in haemostatic variables of pulmonary tuberculosis patients during treatment

    DOI: 10.1016/j.tube.2022.102285
    Sample: serum
  37. JOURNAL OF CLINICAL LABORATORY ANALYSIS (2020) IF: 1.54
    Increased FURIN expression in rheumatoid arthritis patients and its anti‐inflammatory effect

    DOI: 10.1002/jcla.23530
    Sample: Serum
  38. MEDICINE (2020) IF: 1.552
    Regulatory role and mechanism of the inhibition of the Mcl-1 pathway during apoptosis and polarization of H37Rv-infected macrophages

    DOI: 10.1097/MD.0000000000022438

    PMID: 33080678

    		 Sample: Cell culture supernatant
  39. Journal of Obsessive-Compulsive and Related Disorders (2021) IF: 1.677
    TGF-β1, neopterin, tetrahydrobiopterin, and nitric oxide levels in pediatric obsessive–compulsive disorder

    DOI: 10.1016/j.jocrd.2021.100649
    Sample: Serum
  40. Turkish Journal of Biochemistry-Turk Biyokimya Dergisi (2022) IF: 0.481
    Direct-acting antiviral therapy may help restore HCV-induced impaired redox balance and liver fibrosis process

    DOI: 10.1515/tjb-2022-0081
    Sample: serum
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