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Iron Colorimetric Assay Kit

Cat.No.:E-BC-K139-S
Detection instrument: Spectrophotometer(520 nm)

Detection method: Colorimetric method

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Detection principle

Under the action of acidic solution and reductant, ferric ions can be separated from transferrin in serum, and reduced into ferrous ions (Fe2+). The latter then bind to bipyridine and form pink complexes. The concentration of iron can be calculated by measuring the OD value at 520 nm indirectly.

Performance characteristics

Synonyms	Fe
Sample type	Serum,tissue
Sensitivity	0.072 mg/L
Detection range	0.072-60 mg/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	50 min
Precision	Average inter-assay CV: 2.500%Average intra-assay CV: 4.600%
Other instruments required	Micropipettor, Vortex mixer, Incubator, Centrifuge
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Images

The serum iron levels were decreased in all MC-LR treatment groups (A), mice with MC-LR exposure developed iron overload in different tissue (B-F).

C Pan et al investigate risk of microcytic anemia through chronic exposure to MC-LR. Iron (Fe) level in mouse serum and tissue were determined using iron colorimetric assay kit (E-BC-K139-S).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.072-60 mg/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Mouse serum	1
10% Mouse liver tissue homogenization	1
10% Rat kidney tissue homogenization	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

EBioMedicine (2022) IF: 11.205
Single cell RNA-seq analysis identifies ferroptotic chondrocyte cluster and reveals TRPV1 as an anti-ferroptotic target in osteoarthritis

DOI: 10.1016/j.ebiom.2022.104258

PMID: 36137413
Sample: OA cartilage
JOURNAL OF CONTROLLED RELEASE (2022) IF: 9.776
A targeted nanomodulator capable of manipulating tumor microenvironment against metastasis

DOI: 10.1016/j.jconrel.2022.06.022

PMID: 35716882
Sample: 4 T1 cell-xenograft
ANTIOXIDANTS & REDOX SIGNALING (2021) IF: 8.401
A novel identified circular RNA, mmu_mmu_circRNA_0000309 involves in Germacrone-mediated the improvement of diabetic nephropathy through regulating ferroptosis by targeting miR-188-3p/GPX4 signaling axis

DOI: 10.1089/ars.2021.0063

Sample: kidney,Primary mouse podocyte MPC5
Cell and Bioscience (2022) IF: 7.133
β-amyloid protein induces mitophagy-dependent ferroptosis through the CD36/PINK/PARKIN pathway leading to blood–brain barrier destruction in Alzheimer’s disease

DOI: 10.1186/s13578-022-00807-5

Sample: pericyte
Frontiers in Oncology (2021) IF: 6.244
Deferoxamine Counteracts Cisplatin Resistance in A549 Lung Adenocarcinoma Cells by Increasing Vulnerability to Glutamine Deprivation-Induced Cell Death.

DOI: 10.3389/fonc.2021.794735

Sample: A549 cell
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2022) IF: 6.291
GPX4 degradation via chaperone-mediated autophagy contributes to antimony-triggered neuronal ferroptosis

DOI: 10.1016/j.ecoenv.2022.113413

PMID: 35305351
Sample: PC12 cell
Food & Function (2022) IF: 6.317
Arthrospira platensis (Spirulina) fortified functional foods ameliorates iron and protein malnutrition by improving growth, modulating oxidative stress and gut microbiota in rats

DOI: 10.1039/D2FO02226E

Sample: serum
International Journal of Nanomedicine (2022) IF: 6.4
A Composite Deferoxamine/Black Phosphorus Nanosheet/Gelatin Hydrogel Scaffold for Ischemic Tibial Bone Repair

DOI: 10.2147/IJN.S351814

PMID: 35299865
Sample: BPN-DFO hydrogel
Nutrients (2022) IF: 6.706
Iron Depletion in Systemic and Muscle Compartments Defines a Specific Phenotype of Severe COPD in Female and Male Patients: Implications in Exercise Tolerance

DOI: 10.3390/nu14193929

PMID: 36235581
Sample: Muscle
FEBS Journal (2021) IF: 5.542
Interferon-γ induces retinal pigment epithelial cell Ferroptosis by a JAK1-2/STAT1/SLC7A11 signaling pathway in Age-related Macular Degeneration

DOI: 10.1111/febs.16272

PMID: 34741776
Sample: eye
TOXICOLOGY (2020) IF: 4.099
Inhibition of ferroptosis attenuates busulfan-induced oligospermia in mice

DOI: 10.1016/j.tox.2020.152489

Sample: testicular tissue
BIOLOGY OF REPRODUCTION (2021) IF: 4.285
Chronic overload of concentration-dependent iron exerts different effects on ovarian function in C57BL/6J mice

DOI: 10.1093/biolre/ioab040

Sample: serum，ovary
MOLECULAR IMMUNOLOGY (2021) IF: 4.407
Hydrogen sulfide attenuates ferroptosis and stimulates autophagy by blocking mTOR signaling in sepsis-induced acute lung injury

DOI: 10.1016/j.molimm.2021.12.003

PMID: 34952420
Sample: lung tissue
EUROPEAN JOURNAL OF PHARMACOLOGY (2021) IF: 4.432
Dauricine alleviated secondary brain injury after intracerebral hemorrhage by upregulating GPX4 expression and inhibiting ferroptosis of nerve cells

DOI: 10.1016/j.ejphar.2021.174461

PMID: 34469757
Sample: brain tissue
Animals (2022) IF: 3.231
Effect of Zearalenone-Induced Ferroptosis on Mice Spermatogenesis

DOI: 10.3390/ani12213026

Sample: testicular tissue
Bioengineered (2022) IF: 3.269
Amentoflavone promotes ferroptosis by regulating reactive oxygen species (ROS) /5’AMP-activated protein kinase (AMPK)/mammalian target of rapamycin (mTOR) to inhibit the malignant progression of endometrial carcinoma cells

DOI: 10.1080/21655979.2022.2079256

Sample: KLE cell
VETERINARY RESEARCH (2020) IF: 3.357
Identification of ireA, 0007, 0008, and 2235 as TonB-dependent receptors in the avian pathogenic Escherichia coli strain DE205B

DOI: 10.1186/s13567-020-0734-z
NEUROCHEMICAL RESEARCH (2022) IF: 3.996
Epigallocatechin-3-Gallate Ameliorated Iron Accumulation and Apoptosis and Promoted Neuronal Regeneration and Memory/Cognitive Functions in the Hippocampus Induced by Exposure to a Chronic High-Altitude Hypoxia Environment

DOI: 10.1007/s11064-022-03611-2

PMID: 35552996
Sample: hippocampus

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