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KCNK18 Polyclonal Antibody

Cat:E-AB-18168
Manual MSDS

Price: $ 399

Price: $ 240

Price: $ 143

Price: $ 73

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human; Mouse
  • Applications: WB;IHC
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Product Details
Verified Samples Verified Samples in WB:TM4
Verified Samples in IHC:Human liver cancer
Dilution

WB 1:500-1:2000, IHC 1:70-1:350

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Synthetic peptide of human KCNK18
Abbre KCNK18
Synonyms K2p18.1;KCNK18;KCNKI;MGR13;OTTHUMP00000020575;Potassium channel subfamily K member 18;TRESK 2;TRESK;TRESK2;TRIK;TWIK related individual K+ channel;TWIK related individual potassium channel;TWIK related spinal cord K+ channel ;TWIK related spinal cord potassium channel;TWIK-related individual potassium channel;TWIK-related spinal cord potassium channel
Swissprot
Calculated MW 44 kDa
Observed MW Refer to figures
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cell membrane.
Concentration 2.28 mg/mL
Buffer PBS with 0.05% NaN3 and 40% Glycerol,pH7.4
Purification Method Antigen affinity purification
Research Areas Cancer; Neuroscience; Signal transduction
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background Potassium channels play a role in many cellular processes including maintenance of the action potential, muscle contraction, hormone secretion, osmotic regulation, and ion flow. This gene encodes a member of the superfamily of potassium channel proteins containing two pore-forming P domains and the encoded protein functions as an outward rectifying potassium channel. A mutation in this gene has been found to be associated with migraine with aura.