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L-Lactic Acid (LA) Colorimetric Assay Kit (Whole Blood Samples)

Name: L-Lactic Acid (LA) Colorimetric Assay Kit (Whole Blood Samples)
Brand: Elabscience
SKU: E-BC-K043-S
Price: 300 USD
Availability: InStock
Rating: 5 (1 reviews)

Citations(2)

Cat.No.:E-BC-K043-S
Detection instrument: Spectrophotometer(530 nm)

Detection method: Colorimetric method

Manual MSDS

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Detection principle

Using NAD+ as hydrogen acceptor, LDH catalyzes the conversion of both lactate and NAD+ into pyruvic acid and NADH respectively. 1-Methoxy-5-methyl phenazine methyl sulfate (PMS) transfers hydrogen from NADH to NBT which deoxidize into purple chromogenic substrate. Lactic acid content can be calculated by measuring the OD value at 530 nm.

Performance characteristics

Sample type	Whole blood
Sensitivity	0.14 mmol/L
Detection range	0.14-7.0 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	60 min
Precision	Average inter-assay CV: 2%Average intra-assay CV: 1.600%
Other instruments required	Micropipettor, Vortex mixer, Incubator, Centrifuge
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.14-7.0 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Rabbit whole blood	1-2
Mouse whole blood	1-2
Rat whole blood	1-2

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

BIOCHEMICAL PHARMACOLOGY (2020) IF: 4.96
Canagliflozin inhibits p-gp function and early autophagy and improves the sensitivity to the antitumor effect of doxorubicin

DOI: 10.1016/j.bcp.2020.113856

PMID: 32061772
Sample: Cell lysate
Biomedical Engineering Online (2021) IF: 2.819
Ribonucleic acid-binding protein CPSF6 promotes glycolysis and suppresses apoptosis in hepatocellular carcinoma cells by inhibiting the BTG2 expression

DOI: 10.1186/s12938-021-00903-6

PMID: 34217312
Sample: Huh-8 cells

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Verified Customer

B***aSubmitted [ May 31 2019 ]

Application:Biochemical
Description:The kit is simple to operate and the reaction time is short, but it requires rapid detection after reaction. In general, the biochemical kit is good

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