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L-Lactic Acid (LA) Colorimetric Assay Kit

Name: L-Lactic Acid (LA) Colorimetric Assay Kit
Brand: Elabscience
SKU: E-BC-K044-S
Price: 300 USD
Availability: InStock
Rating: 5 (2 reviews)

Citations(10)

Cat.No.:E-BC-K044-S
Detection instrument: Spectrophotometer(530 nm)

Detection method: Colorimetric method

Manual MSDS

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Detection principle

Using NAD+ as hydrogen acceptor, LDH catalyzes the conversion of both lactate and NAD+ into pyruvic acid and NADH respectively. 1-Methoxy-5-methyl phenazine methyl sulfate (PMS) transfers hydrogen from NADH to NBT which deoxidize into purple chromogenic substrate. Lactic acid content can be calculated by measuring the OD value at 530 nm.

Performance characteristics

Sample type	Serum,plasma,cell culture supernatant,tissue,cells
Sensitivity	0.05 mmol/L
Detection range	0.05-6.0 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	60 min
Precision	Average inter-assay CV: 1.900%Average intra-assay CV: 1.100%
Other instruments required	Micropipettor, Vortex mixer, Incubator, Centrifuge
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Images

DOX administration significantly increased hippocampal lactic acid content (*P<0.05 vs. Control group), AML co-treatment significantly lowered hippocampal lactic acid content to near control levels. AML alone treated rats showed no significant difference when compared to control group. (#P<0.05 and ###P<0.001 vs. DOX group)

A E Ali investigate the antitumor effects of AML and its ability to mitigate cancer resistance to DOX therapy. L-Lactate (L-LA) of rat hippocampal was determined using L-lactate colorimetric assay kit (E-BC-K044-S).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.05-6.0 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	4-8
10% Mouse muscle tissue homogenization	2-4
10% Mouse liver tissue homogenization	1
Rat serum	4-8
HePG2 cells homogenization (1.388 gprot/L)	4-8
HepG2 supernatant	2-4

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Cell Communication and Signaling (2022) IF: 7.525
Inhibition of AMPK/PFKFB3 mediated glycolysis synergizes with penfluridol to suppress gallbladder cancer growth

DOI: 10.1186/s12964-022-00882-8

PMID: 35842652
Sample: SGC-996 cell,EH-GB1 cell,GBC-SD cell
CANCER SCIENCE (2022) IF: 6.518
circ_0006089 promotes gastric cancer growth, metastasis, glycolysis, and angiogenesis by regulating miR‐361‐3p/TGFB1

DOI: 10.1111/cas.15351

Sample: AGS cell,HGC-27 cell
iScience (2021) IF: 5.458
Excipient of paclitaxel induces metabolic dysregulation and unfolded protein response

DOI: 10.1016/j.isci.2021.103170

PMID: 34646996
Sample: THP-1 cell
NEUROTOXICOLOGY (2021) IF: 4.294
The diuretic amiloride attenuates doxorubicin-induced chemobrain in rats: Behavioral and mechanistic study

DOI: 10.1016/j.neuro.2021.10.002

PMID: 34656704
Sample: hippocampus
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2023) IF: 3.094
Metabolic Phenotype Intricacies on Altered Glucose Metabolism of Breast Cancer Cells upon Glut-1 Inhibition and Mimic Hypoxia In Vitro

DOI: 10.1007/s12010-023-04373-5

Sample: SKBR-3 breast cancer cell
Bioengineered (2021) IF: 3.269
KLF9 (Kruppel Like Factor 9) induced PFKFB3 (6-Phosphofructo-2-Kinase/Fructose-2, 6-Biphosphatase 3) downregulation inhibits the proliferation, metastasis and aerobic glycolysis of cutaneous squamous cell carcinoma cells

DOI: 10.1080/21655979.2021.1980644

PMID: 34612136
Sample: A431 cell
ACS Omega (2022) IF: 3.512
Specific Tumor Cell Detection by a Metabolically Targeted Aggregation-Induced Emission-Based Gold Nanoprobe

DOI: 10.1021/acsomega.2c01494

Sample: MCF-7 cell,HepG2 cell,Caco-2 cell
Biomedical Engineering Online (2021) IF: 2.819
Ribonucleic acid-binding protein CPSF6 promotes glycolysis and suppresses apoptosis in hepatocellular carcinoma cells by inhibiting the BTG2 expression

DOI: 10.1186/s12938-021-00903-6

PMID: 34217312
Sample: Huh-8 cells
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES (2022) IF: 2.945
CircCDC6 restrains tumor growth and glycolysis energy metabolism in colorectal cancer via regulating miR-3187-3p and downstream PRKAA2

DOI: 10.1007/s10863-022-09938-3

PMID: 35438362
Sample: SW480 cell,HCT116 cell
Molecular Medicine Reports (2021) IF: 2.952
circ-PRKCI targets miR-1294 and miR-186-5p by downregulating FOXK1 expression to suppress glycolysis in hepatocellular carcinoma

DOI: 10.3892/mmr.2021.12103

Sample: Cell sample(HCCLM3)

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Reviews/Q&A

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Q&A

Verified Customer

p****uSubmitted [ Jun 19 2019 ]

Application:Biochemical
Description:This kit can be fast, reaction only takes 10 minutes, can accurately get the experimental results, less interference factors. I use this kit to detect human serum, each sample is tested three times, the difference between the results of samples is small, and all samples have a good trend. So the quality of this kit is very good.

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Verified Customer

C***ySubmitted [ May 31 2019 ]

Application:Biochemical
Description:This biochemical kit is good, easy and fast. with a small sample size, but it needs fast detection

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M*****iSubmitted [ Oct 21 2022 ]

Asked: Why did my result exceed the detection range of the kit?

adminSubmitted [ Oct 21 2022 ]

Answered: If the sample content is beyond detection range, please dilute the sample before detection.

M*****iSubmitted [ Sep 13 2022 ]

Asked: What is the homogenate medium of the tissue sample?

adminSubmitted [ Sep 13 2022 ]

Answered: You can choose PBS (0.01 M, pH 7.4) or 0.9% NaCl.

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