The Long-arm Biotin Labeling Kit of Elabscience offering a collection of reagents required for biotin labeling is designed to label antibody with amidogen (NH2-). Long-arm reduces the steric hindrance when several biotinylated molecules combine with the same avidin molecular compound, effectively optimizes the labeling and detection results. The biotin in this kit is sufficient and has been activated for direct use. The reagents are enough for approximately 3 labeling reactions each containing 0.1-2mg of antibody or other protein. Each kit includes 6 Filtration tubes for desalination of antibody labeling without the need for dialysis. The whole procedure is simple and can be completed in 90min with proficient operation.
Information of the Long-arm
Molecular Weight: 568
Molecular formula: C26H41N5O7S
Spacer arm length: 30.5 Å
1. All-inclusive: This kit provides all the reagents required for biotin labeling.
2. Quick: The whole procedure takes only 90 min.
3. Convenient: Desalination can be achieved with Filtration tube, dialysis or gel filtration is not necessary.
4. Flexible: The procedure can be easily adapted to both micro and large scales, with 0.1-2 mg of protein labeled each time.
Perfect results: This kit has been optimized to determine the optimum labeling ratio of biotin to antibody, lowering the possibility of protein inactivation resulted from insufficient labeling or excess biotin labeling.
The NH2-Reactive Biotin reacts with the primary amine (N-terminal and the side chain of lysine residue) specifically, forming stable amide bond.