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Low-density Lipoprotein Cholesterol (LDL-C) Colorimetric Assay Kit (Double Reagents)

Cat.No.:E-BC-K205
Detection instrument: Microplate reader(546 nm), Biochemistry analyzer(546 nm)

Detection method: Colorimetric method

To Purchase E-BC-K205

Size:

96T

Price: $150

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Protocols for metabolism assays

FAQ for metabolism assays

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For research use only. Order now, ship in 3 days

Detection principle

The coloured substance have a maximum absorption peak at 546 nm. Measure the OD value at 546 nm and the LDL-C content in the sample can be calculated.

Performance characteristics

Synonyms	LDL-C
Sample type	Serum,plasma,cells,culture supernatant,tissue
Detection range	0.2-12 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	20 min
Storage	2-8℃
Valid period	12 months

Images

LDL-C levels in serum and hippocampal tissue were significantly increased in OVX/HFFD/D-Gal group, and decreased in perindopril treatment group. (a. significant change with normal control; b. significant change with OVX/HFFD/D-Gal)

B A S Messiha et al found that perindopril ameliorates Alzheimer’s disease progression. Low density lipoprotein cholesterol (LDL-C) levels in rat serum and hippocampus were determined using LDL-C colorimetric assay kit (E-BC-K205).

Ralated product

Cat Number	Product Name
E-BC-K238	Triglyceride (TG) Colorimetric Assay Kit (Single Reagent, GPO-PAP Method)
E-BC-K109-M	Total Cholesterol (TC) Colorimetric Assay Kit (Single Reagent, COD-PAP Method)
E-BC-K221	High-density Lipoprotein Cholesterol (HDL-C) Colorimetric Assay Kit (Double Reagents)

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2023) IF: 8.025
Scavenger receptor a mediates glycated LDL transcytosis across endothelial cells to promote atherosclerosis

DOI: 10.1016/j.ijbiomac.2023.123836

Sample: blood
Food & Function (2023) IF: 6.317
Analysis of the mechanism of action of quercetin in the treatment of hyperlipidemia based on metabolomics and intestinal flora

DOI: 10.1039/D2FO03509J

Sample: serum
Frontiers in Cell and Developmental Biology (2021) IF: 6.684
N6-Methyladenosine Methyltransferase METTL3 Promotes Angiogenesis and Atherosclerosis by Upregulating the JAK2/STAT3 Pathway via m6A Reader IGF2BP1.

DOI: 10.3389/fcell.2021.731810

Sample: plasma
CELL BIOLOGY AND TOXICOLOGY (2022) IF: 6.691
ATGL deficiency aggravates pressure overload-triggered myocardial hypertrophic remodeling associated with the proteasome-PTEN-mTOR-autophagy pathway

DOI: 10.1007/s10565-022-09699-0

PMID: 35218467
Sample: serum
EUROPEAN JOURNAL OF PHARMACOLOGY (2023) IF: 5.195
Pancreastatin inhibitor PSTi8 ameliorates streptozotocin-induced diabetes by suppressing hepatic glucose production

DOI: 10.1016/j.ejphar.2023.175559

Sample: plasma
JOURNAL OF ETHNOPHARMACOLOGY (2022) IF: 5.195
Integrating metabolomics and network pharmacology to investigate Panax japonicus prevents kidney injury in HFD/STZ-induced diabetic mice

DOI: 10.1016/j.jep.2022.115893

Sample: serum
Food Bioscience (2022) IF: 5.318
Phytosterol of lotus seed core powder alleviates hypercholesterolemia by regulating gut microbiota in high-cholesterol diet-induced C57BL/6J mice

DOI: 10.1016/j.fbio.2022.102279

Sample: serum,liver tissue
Food Bioscience (2022) IF: 5.318
Ameliorative effects of monascin from red mold rice on alcoholic liver injury and intestinal microbiota dysbiosis in mice

DOI: 10.1016/j.fbio.2022.102079

Sample: serum
Foods (2022) IF: 5.561
Monascuspiloin from Monascus-Fermented Red Mold Rice Alleviates Alcoholic Liver Injury and Modulates Intestinal Microbiota

DOI: 10.3390/foods11193048

PMID: 36230124
Sample: serum
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022) IF: 5.924
The Activity of Ten Natural Extracts Combined in a Unique Blend to Maintain Cholesterol Homeostasis—In Vitro Model

DOI: 10.3390/ijms23073805

Sample: HepG2 cell
ACS Omega (2022) IF: 4.132
Beneficial Effects of lncRNA-UC.360+ shRNA on Diabetic Cardiac Sympathetic Damage via NLRP3 Inflammasome-Induced Pyroptosis in Stellate Ganglion

DOI: 10.1021/acsomega.2c03619

PMID: 35967043
Sample: serum
TOXICOLOGY LETTERS (2021) IF: 4.374
Alpha-lipoic acid attenuates silica-induced pulmonary fibrosis by improving mitochondrial function via AMPK/PGC1α pathway activation in C57BL/6J mice

DOI: 10.1016/j.toxlet.2021.07.003

PMID: 34252510
Sample: serum
JOURNAL OF PSYCHIATRIC RESEARCH (2022) IF: 4.791
Comparison of cognitive impairments with lipid profiles and inflammatory biomarkers in unipolar and bipolar depression

DOI: 10.1016/j.jpsychires.2022.04.002

PMID: 35429740
Sample: serum
Diabetes Metabolic Syndrome and Obesity-Targets and Therapy (2022) IF: 3.168
Serum Levels of Free Fatty Acids in Obese Mice and Their Associations with Routine Lipid Profiles

DOI: 10.2147/DMSO.S348800

PMID: 35140491
Sample: serum
MEAT SCIENCE (2019) IF: 3.483
Effects of naturally-produced lovastatin on carcass characteristics, muscle physico-chemical properties and lipid oxidation and cholesterol content in goats

DOI: 10.1016/j.meatsci.2019.04.008

Sample: serum
LIFE SCIENCES (2020) IF: 3.647
Naringin ameliorates type 2 diabetes mellitus-induced steatohepatitis by inhibiting RAGE/NF-κB mediated mitochondrial apoptosis

DOI: 10.1016/j.lfs.2020.118118

Sample: serum
BIOMEDICINE & PHARMACOTHERAPY (2019) IF: 3.743
Pancreastatin inhibitor activates AMPK pathway via GRP78 and ameliorates dexamethasone induced fatty liver disease in C57BL/6 mice

DOI: 10.1016/j.biopha.2019.108959

Sample: plasma
Heliyon (2020) IF: 3.776
PSTi8 with metformin ameliorates perimenopause induced steatohepatitis associated ER stress by regulating SIRT-1/SREBP-1c axis

DOI: 10.1016/j.heliyon.2020.e05826

Sample: plasma
THERIOGENOLOGY (2020) IF: 2.094
Diet-induced obesity is associated with altered expression of sperm motility-related genes and testicular post-translational modifications in a mouse model

DOI: 10.1016/j.theriogenology.2020.09.023

PMID: 32980686
Sample: Serum
Journal of Developmental Origins of Health and Disease (2020) IF: 2.456
Neonatal zingerone protects against the development of high-fructose diet-induced metabolic syndrome in adult Sprague-Dawley rats

DOI: 10.1017/S2040174420000525

Sample: plasma
International Journal of Clinical and Experimental Pathology (2018) IF: 1.396
Pitavastatin attenuates atherosclerosis by suppressing NF-κB signaling in a high-cholesterol diet plus balloon catheter injury rabbit model

PMID: 31938118
Sample: serum
TOXICOLOGY AND INDUSTRIAL HEALTH (2020) IF: 1.708
Elevated, sustained, and yet reversible biotoxicity effects of lead on cessation of exposure: Melatonin is a potent therapeutic option:

DOI: 10.1177/0748233720937199

Sample: Plasma
BMC Veterinary Research (2020) IF: 1.835
Tea polyphenols attenuate liver inflammation by modulating obesity-related genes and down-regulating COX-2 and iNOS expression in high fat-fed dogs

DOI: 10.1186/s12917-020-02448-7

PMID: 32641048
Sample: Serum,Tissue homogenate

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Reviews/Q&A

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Reviews
Q&A

M*****aSubmitted [ Oct 20 2022 ]

Asked: What cell culture media and composition would you recommend for LDL assays. I would like to look at the cell culture supernatant. Thank you

adminSubmitted [ Oct 20 2022 ]

Answered: Sorry, our LDL Assay Kit can not be used on cell culture supernatant.

M*****aSubmitted [ Sep 16 2022 ]

Asked: What is meant by double reagent?

adminSubmitted [ Sep 16 2022 ]

Answered: Double reagent means that there are only two reagents in this kit: reagent 1 and reagent 2.

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