Current Position:Home>>Kits>>Metabolism Assays>> Magnesium (Mg) Colorimetric Assay Kit

Magnesium (Mg) Colorimetric Assay Kit

Cat.No.:E-BC-K162-M
Detection instrument: Microplate reader(520-550 nm,optimum wavelength: 540 nm)

Detection method: Colorimetric method

To Purchase E-BC-K162-M

Size:

96T
48T

Price: $200

Qty:

Welcome to order from local distributors.

Protocols for metabolism assays

FAQ for metabolism assays

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Detection principle

The magnesium in the serum reacts with the complexometric indicator (Calmagite) to form the Calmagite-Mg compound. The absorbance of this compound at 540 nm is proportional to the concentration of magnesium in the sample. The concentration of magnesium can be calculated by measuring the OD value at 540 nm.

Performance characteristics

Synonyms	Mg
Sample type	Serum,plasma
Sensitivity	0.18 mmol/L
Detection range	0.18-2.50 mmol/L
Detection method	Colorimetric method
Assay type	Quantitative
Assay time	20 min
Precision	Average inter-assay CV: 7.900%Average intra-assay CV: 5.100%
Other instruments required	Micropipettor, Centrifuge, Incubator, Vortex mixer
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.18-2.50 mmol/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Rat serum	1
Mouse serum	1
Porcine serum	1
Chicken serum	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Frontiers in Plant Science (2022) IF: 6.627
Halotolerant Bacillus altitudinis WR10 improves salt tolerance in wheat via a multi-level mechanism

DOI: 10.3389/fpls.2022.941388

PMID: 35909740
Sample: roots or shoots

Reviews/Q&A

Show all
Reviews
Q&A

... Show All Show Less

Cat. No	E-BC-K162-M
Application
Sample Type

*Product Name:
*Catalog Number:
*Name:
*Email:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha