Current Position:Home>>Kits>>ELISA Kits>> Mouse TRACP-5b(Tartrate Resistant Acid Phosphatase 5b) ELISA Kit

Mouse TRACP-5b(Tartrate Resistant Acid Phosphatase 5b) ELISA Kit

Citations(14)Uniprot : Q05117

Cat.No.:E-EL-M1116
Reactivity: Mouse

Filter:

ELISA

To Purchase E-EL-M1116

Size:

96T
48T
24T
96T*5
96T*10

Price: $609

Qty:

Welcome to order from local distributors.

Protocols for ELISA

FAQ for ELISA

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.78-50 ng/mL
Sensitivity	0.47 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Mouse TRACP-5b in samples. No significant cross-reactivity or interference between Mouse TRACP-5b and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Mouse TRACP-5b concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TRACP-5b. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TRACP-5b and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TRACP-5b, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TRACP-5b. You can calculate the concentration of Mouse TRACP-5b in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse TRACP-5b were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse TRACP-5b were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	2.52	5.33	22.43	2.70	4.86	21.67
Standard deviation	0.15	0.26	1.11	0.18	0.24	0.75
CV (%)	5.95	4.88	4.95	6.67	4.94	3.46

Recovery

The recovery of Mouse TRACP-5b spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	94-108	99
EDTA plasma (n=8)	89-102	96
Cell culture media (n=8)	93-103	98

Linearity

Samples were spiked with high concentrations of Mouse TRACP-5b and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: Q05117
Research Area	Cancer, Metabolism, Immunology, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Journal of the American Chemical Society (2020) IF: 14.612
Smart Nanosacrificial Layer on the Bone Surface Prevents Osteoporosis through Acid–Base Neutralization Regulated Biocascade Effects

DOI: 10.1021/jacs.0c07309

PMID: 32960592
Sample: Serum
Redox Biology (2021) IF: 11.799
Sirt3-mediated mitophagy regulates AGEs-induced BMSCs senescence and senile osteoporosis

DOI: 10.1016/j.redox.2021.101915

PMID: 33662874
Sample: Serum
Acta Biomaterialia (2022) IF: 10.633
Genesis of osteoclasts on calcium phosphate ceramics and their role in material-induced bone formation

DOI: 10.1016/j.actbio.2022.11.005
CELL PROLIFERATION (2023) IF: 8.7550
Dietary nitrate improves jaw bone remodelling in zoledronate-treated mice

DOI: 10.1111/cpr.13395

Sample: Plasma,tooth sockets
BIOMEDICINE & PHARMACOTHERAPY (2022) IF: 7.419
Thiaplakortone B attenuates RANKL‐induced NF‐κB and MAPK signaling and dampens OVX‐induced bone loss in mice

DOI: 10.1016/j.biopha.2022.113622

Sample: serum
Oncotarget (2017) IF: 5.168
Dectin-1 signaling inhibits osteoclastogenesis via IL-33-induced inhibition of NFATc1

DOI: 10.18632/oncotarget.18411

Sample: Bone marrow cells (BMCs)
Bone & Joint Research (2022) IF: 5.853
N,N-Dimethylformamide inhibits high glucose-induced osteoporosis via attenuating MAPK and NF-κB signalling

DOI: 10.1302/2046-3758.114.BJR-2020-0308.R2

Sample: plasma
JOURNAL OF BONE AND MINERAL RESEARCH (2020) IF: 5.854
Hymenialdisine: A Marine Natural Product That Acts on Both Osteoblasts and Osteoclasts and Prevents Estrogen-Dependent Bone Loss in Mice

DOI: 10.1002/jbmr.4025

Sample: serum
STEM CELLS (2016) IF: 5.902
Brief Report: Consecutive Alendronate Administration‐Mediated Inhibition of Osteoclasts Improves Long‐Term Engraftment Potential and Stress Resistance of HSCs

DOI: 10.1002/stem.2425

Sample: Serum
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021) IF: 5.924
Aromatic Bis[aminomethylidenebis(phosphonic)] Acids Prevent Ovariectomy-Induced Bone Loss and Suppress Osteoclastogenesis in Mice

DOI: 10.3390/ijms22179590

PMID: 34502499
Sample: plasma
Aging and Disease (2019) IF: 4.232
Calcitriol Analogues Decrease Lung Metastasis but Impair Bone Metabolism in Aged Ovariectomized Mice Bearing 4T1 Mammary Gland Tumours

DOI: 10.14336/AD.2018.0921

Sample: serum
BONE (2021) IF: 4.398
Coupling Induction of Osteogenesis and Type H Vessels by Pulsed Electromagnetic Fields in Ovariectomy-induced Osteoporosis in Mice

DOI: 10.1016/j.bone.2021.116211

PMID: 34560308
Sample: serum
CHEMICO-BIOLOGICAL INTERACTIONS (2017) IF: 3.143
Methoxsalen supplementation attenuates bone loss and inflammatory response in ovariectomized mice

DOI: 10.1016/j.cbi.2017.10.014

Sample: Serum
Journal of Ayurveda and Integrative Medicine (2018)
Eurycoma longifolia, a promising suppressor of RANKL-induced differentiation and activation of osteoclasts: An in vitro mechanistic evaluation

DOI: 10.1016/j.jaim.2017.07.014

Sample: RAW 264.7 cells

Show AllShorten

Reviews/Q&A

Show all
Reviews
Q&A

... Show All Show Less

*Product Name:
*Catalog Number:
Size:
*Name:
*Email:
*Phone:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha