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Mouse Ang-Ⅱ(Angiotensin Ⅱ) ELISA Kit

Citations(7)Uniprot : P11859

Cat.No.:E-EL-M2612
Reactivity: Mouse

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Product Details

Properties

Assay type	Competitive-ELISA
Format	96T/48T
Assay time	2.0h
Detection range	15.63-1000 pg/mL
Sensitivity	9.38 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 50μL
Specificity	This kit recognizes Ang-Ⅱ in samples. No significant cross-reactivity or interference between Ang-Ⅱ and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Ang-Ⅱ concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Ang-Ⅱ. During the reaction, Ang-Ⅱ in the sample or standard competes with a fixed amount of Ang-Ⅱ on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Ang-Ⅱ. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Ang-Ⅱ in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8℃
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Ang-Ⅱ were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Ang-Ⅱ were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	46.00	138.00	442.80	42.50	130.80	407.70
Standard deviation	2.40	8.10	22.10	2.70	6.90	17.50
CV (%)	5.22	5.87	4.99	6.35	5.28	4.29

Recovery

The recovery of Ang-Ⅱ spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	92-105	100
EDTA plasma(n=8)	85-101	92
Cell culture media(n=8)	86-100	91

Target Information

Database Links	SwissProt: P11859
Research Area	Cancer, Cardiovascular, Metabolism, Signal transduction

Assay Procedures

	1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
	2. Aspirate and wash the plate for 3 times
	3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	5. Add 50μL Stop Solution
	6. Read the plate at 450nm immediately. Calculation of the results

Citations

HYPERTENSION (2019) IF: 7.017
Maternal High-Fructose Intake Induces Multigenerational Activation of the Renin-Angiotensin-Aldosterone System

DOI: 10.1161/HYPERTENSIONAHA.119.12941

PMID: 31327271
Sample: Serum
eLife (2018) IF: 7.616
Arterial smooth muscle cell PKD2 (TRPP1) channels regulate systemic blood pressure

DOI: 10.7554/eLife.42628

PMID: 30511640
Sample: Plasma
FASEB JOURNAL (2021) IF: 5.192
Apela inhibits systemic and renal inflammatory reactions in mice with type I cardiorenal syndrome

DOI: 10.1096/fj.202101030R

PMID: 34516679
Sample: plasma
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2022) IF: 5.31
LncRNA HOX transcript antisense RNA mitigates cardiac function injury in chronic heart failure via regulating microRNA-30a-5p to target KDM3A

DOI: 10.1111/jcmm.17160

PMID: 35083842
Sample: plasma
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE (2022) IF: 4.925
Short-Term Hyperuricemia Leads to Structural Retinal Changes That Can be Reversed by Serum Uric Acid Lowering Agents in Mice

DOI: 10.1167/iovs.63.10.8

PMID: 36098977
Sample: serum
NUTRITION RESEARCH (2020) IF: 2.767
Açaí seed extract prevents the renin-angiotensin system activation, oxidative stress and inflammation in white adipose tissue of high-fat diet–fed mice

DOI: 10.1016/j.nutres.2020.05.006

Sample: Plasma
Nutrition Research and Practice (2020) IF: 1.792
Maternal high-fructose intake during pregnancy and lactation induces metabolic syndrome in adult offspring

DOI: 10.4162/nrp.2021.15.2.160

PMID: 33841721
Sample: serum

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