Mouse D2D(D-Dimer) ELISA Kit

    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    <
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    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience

      Catalog number:E-EL-M0400

      Synonyms:D 2 Dimer

      Size:
      • 96T
      • 24T
      Qty:
      - +
      Price: $609

      Reactivity: Mouse

      Detection Range: 78.13~5000 ng/mL

      Sensitivity: 46.88 ng/mL

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request

      Intended use


      This ELISA kit applies to the in vitro quantitative determination of Mouse D2D concentrations in plasma sample.

      Test principle


      This ELISA kit uses the Competitive-ELISA principle. The microtiter plate provided in this kit has been pre-coated with Mouse D2D. During the reaction, Mouse D2D in the sample or standard competes with a fixed amount of Mouse D2D on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse D2D. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse D2D in the samples is then determined by comparing the OD of the samples to the standard curve.


      Assay type

      Competitive

      Format

      96T

      Assay time

      2.0h

      Reactivity

      Mouse

      Detection Method

      Colormetric

      Detection Range

      78.13— 5000ng/mL

      Sensitivity

       46.88ng/mL

      Sample Volume

      50μL

      Sample Type

      Plasma


      Specificity


      This kit recognizes in samples Mouse D2D. No significant cross-reactivity or interference between Mouse D2D and analogues was observed.



      Kit components & Storage


      An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received


      Item

      Specifications

      Storage

      Micro ELISA Plate (Dismountable)

      8 wells ×12 strips

      -20, 6 months

      Reference Standard

      2 vials

      Concentrated Biotinylated Detection Ab (100×)

      1 vial, 120 uL

      Concentrated HRP Conjugate (100×)

      1 vial, 120 μL

      -20(shading light), 6 months

      Reference Standard & Sample Diluent

      1 vial, 20 mL

      4, 6 months

      Biotinylated Detection Ab Diluent

      1 vial, 14 mL

      HRP Conjugate Diluent

      1 vial, 14 mL

      Concentrated Wash Buffer (25×)

      1 vial, 30 mL

      Substrate Reagent

      1 vial, 10 mL

      4(shading light)

      Stop Solution

      1 vial, 10 mL

      4

      Plate Sealer

      5 pieces

      Product Description

      1 copy

      Certificate of Analysis

      1 copy


      Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.

              The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring 

              equipment instead of directly pouring into the vial(s).


      Other supplies required


      Microplate reader with 450 nm wavelength 

      filter High-precision transfer pipette, EP tubes and disposable pipette tips 

      Incubator capable of maintaining 37℃ 

      Deionized or distilled water 

      Absorbent paper 

      Loading slot for Wash Buffer



      Citations

      1. Publication: Bo Z, Jiang T, She X, et al. Fibrin Degradation By rtPA Enhances The Delivery Of Nanotherapeutics To A549 Tumors In Nude Mice[J]. Biomaterials, 2016, 96: 63-71.
        Species: Mouse
        Sample Type: Tissue homogenates
      2. Publication: SONG Quan Quan, NIU Jing Ping, ZHANG Shu Yu, LIANG Ting Ting, ZHOU Ji, FENG Shan Shan. Effects of Simulated Heat Wave and Ozone on High Fat Diet ApoE Deficient Mice. Biomedical and Environmental Sciences, 2018, 31(10): 757-768.
        Species: Mouse
        Sample Type: Plasma and Heart homogenate
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