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Mouse EGF (Epidermal growth factor) CLIA Kit

Cat:E-CL-M0023
Manual MSDS

Price: $ 682

Price: $ 546

Price: $ 180

Price: Inquire

Price: Inquire

Size:
96T 48T 24T 96T*10 96T*5
Quantity:
  • Reactivity: Mouse
  • Detection Range: 7.81-500 pg/mL
  • Sensitivity: 4.69 pg/mL
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Product Details
Test Principle This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Mouse EGF. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse EGF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse EGF, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Mouse EGF. You can calculate the concentration of Mouse EGF in the samples by comparing the RLU value of the samples to the standard curve.
Assay Type Sandwich-ELISA
size 96T / 48T / 24T / 96T*10 / 96T*5
Assay Time 3.5h
Detection Method Chemiluminescence
Detection Range 7.81-500 pg/mL
Sensitivity 4.69 pg/mL
Sample Volume 100μL
Sample Type serum, plasma and other biological fluids
Specificity This kit recognizes Mouse EGF in samples.No significant cross-reactivity or interference between Mouse EGF and analogues was observed
Precision Both intra-CV and inter-CV are < 10%.
Recovery 80%-120%
Introduction This ELISA kit applies to the in vitro quantitative determination of Mouse EGF concentrations in serum, plasma and other biological fluids.
Applications CLIA
Storage 2-8℃/-20℃
Database SwissProt:  P01132
Syonoyms URG,HOMG4,Beta-Urogastrone
Research Area Cancer Cell Biology Signal Transduction