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Cat.No.:E-CL-M0289
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Assay type | Sandwich |
Format | 96T |
Assay time | 4.5h |
Detection range | 62.50-4000 pg/mL |
Sensitivity | 37.50 pg/mL |
Sample type &Sample volume | Serum, plasma and other biological fluids; 100μL |
Specificity | This kit recognizes Mouse ERα in samples. No significant cross-reactivity or interference between Mouse ERα and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 15%. |
Application | This CLIA kit applies to the in vitro quantitative determination of Mouse ERα concentrations in Serum, plasma and other biological fluids. |
Item | Specifications | Storage |
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Micro CLIA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
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Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
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Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent A | 1 vial, 5 mL | 2-8℃(Protect from light) |
Substrate Reagent B | 1 vial, 5 mL | |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse ERα were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse ERα were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 192.86 | 518.67 | 1894.66 | 178.39 | 525.64 | 1866.66 |
Standard deviation | 19.42 | 46.21 | 143.99 | 16.30 | 47.41 | 213.17 |
CV (%) | 10.07 | 8.91 | 7.60 | 9.14 | 9.02 | 11.42 |
The recovery of Mouse ERα spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 94-107 | 101 |
EDTA plasma(n=8) | 91-104 | 97 |
Cell culture media(n=8) | 86-97 | 91 |
Synonyms | ESR1, ER, ESR, ESRA, Era, NR3A1, nuclear receptor subfamily 3, group A, member 1 |
Research Area | Cancer,Neuroscience,Epigenetics and Nuclear Signaling |
1.Add 100 μL of standard or sample to each well. Incubate for 90 min at 37℃. |
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2.Remove the liquid. |
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3.Add 100 μL Biotinylated Detection Ab. Incubate 1 hour at 37℃. Aspirate and wash 3 times. |
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4.Add 100 μL HRP Conjugate. Incubate 30 min at 37℃. Aspirate and wash 5 times. |
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5.Add 100 μL Substrate Mixture Solution. Incubate for 5 min at 37℃. |
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6.Fluorescence appeared. Measure the RLU value with the Chemiluminescence immunoassay analyzer. Calculation of the results. |