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Mouse hs-CRP(high-sensitivity C-Reactive Protein) ELISA Kit

Cat.No.:E-EL-M0677
Reactivity: Mouse

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96T*10

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Properties

Format	24T, 48T, 96T, 96T5, 96T10
Assay time	3.5h
Sample type	serum, plasma and other biological fluids
Sample volume	100μL
Sample size	Run up to 46 samples in duplicate and 30 samples in triplicate
Storage	2-8℃
Interpretation	Detection range: 6.86-5000 pg/mL Sensitivity: 4.12 pg/mL
Application	Quantitative determination of Human concentrations in human serum, plasma and other biological fluids
Reproducibility	Both intra-CV and inter-CV are < 10%

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse hs-CRP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse hs-CRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse hs-CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse hs-CRP. You can calculate the concentration of Mouse hs-CRP in the samples by comparing the OD of the samples to the standard curve.

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

FASEB JOURNAL (2021) IF: 5.192
Apela inhibits systemic and renal inflammatory reactions in mice with type I cardiorenal syndrome

DOI: 10.1096/fj.202101030R

PMID: 34516679
Sample: plasma
Frontiers in Aging Neuroscience (2022) IF: 5.702
Transplantation of fecal microbiota from APP/PS1 mice and Alzheimer’s disease patients enhanced endoplasmic reticulum stress in the cerebral cortex of wild-type mice

DOI: 10.3389/fnagi.2022.858130

Sample: serum
INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 5.714
Menthone inhibits type-I interferon signaling by promoting Tyk2 ubiquitination to relieve local inflammation of rheumatoid arthritis

DOI: 10.1016/j.intimp.2022.109228

PMID: 36095947
Sample: serum
American Journal of Translational Research (2018) IF: 4.06
Apolipoprotein B-100 peptide 210 antibody inhibits atherosclerosis by regulation of macrophages that phagocytize oxidized lipid

PMID: 30018722
Sample: plasma
STEM CELLS AND DEVELOPMENT (2022) IF: 4.39
Alteration of transcriptomic profile and anti-septic efficacy of adipose-derived mesenchymal stromal/stem cells under different culture conditions

DOI: 10.1089/scd.2022.0238

Sample: plasma

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