Mouse IFN-γ(Interferon Gamma) ELISA Kit

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    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
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    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience

      Catalog number:E-EL-M0048

      Synonyms:IFNG, IFG, IFI, Type II Interferon

      Size:
      • 96T
      • 24T
      Qty:
      - +
      Price: $495

      Reactivity: Mouse

      Detection Range: 15.63~1000 pg/mL

      Sensitivity: 9.38 pg/mL

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request

      Intended use

      This ELISA kit applies to the in vitro quantitative determination of Mouse IFN-γ concentrations in Serum, plasma and other biological fluids.

      Test principle

      This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IFN-γ . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IFN-γ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IFN-γ, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IFN-γ. You can calculate the concentration of Mouse IFN-γ in the samples by comparing the OD of the samples to the standard curve.

      Assay type Sandwich
      Format 96T
      Assay time 3.5h
      Reactivity Mouse
      Detection Method Colormetric
      Detection Range 15.63—1000 pg/mL
      Sensitivity 9.38 pg/mL
      Sample Volume Required Per Well 100μL
      Sample Type Serum, plasma and other biological fluids

      Specificity

      This kit recognizes Mouse IFN-γ in samples. No significant cross-reactivity or interference between Mouse IFN-γ and analogues was observed.

      Typical data

      As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

      Concentration
      (pg/mL)
      O.D Average Corrected
      1000 2.384
      2.396
      2.39 2.318
      500 1.575
      1.589
      1.582 1.51
      250 0.984
      0.974
      0.979 0.907
      125 0.427
      0.459
      0.443 0.371
      62.5 0.237
      0.235
      0.236 0.164
      31.25 0.184
      0.162
      0.173 0.101
      15.63 0.12
      0.128
      0.124 0.052
      0 0.065
      0.079
      0.072 --

      Precision

      Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IFN-γ were tested 20 times on one plate, respectively.
      Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IFN-γ were tested on 3 different plates, 20 replicates in each plate.

      Intra-assay Precision Inter-assay Precision
      Sample 1 2 3 1 2 3
      n 20 20 20 20 20 20
      Mean (pg/mL) 51.50 96.50 497.20 53.30 95.50 533.30
      Standard deviation 3.20 4.40 20.90 3.70 4.70 17.10
      C V (%) 6.21 4.56 4.20 6.94 4.92 3.21

      Recovery

      The recovery of Mouse IFN-γ spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

      Sample Type Range (%) Average Recovery (%)
      Serum (n=5) 89-103 96
      EDTA plasma (n=5) 91-103 98
      Cell culture media (n=5) 96-109 102

      Linearity

      Samples were spiked with high concentrations of Mouse IFN-γ and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

      Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
      1:2 Range (%) 88-98 86-98 89-102
      Average (%) 93 91 95
      1:4 Range (%) 97-111 87-98 94-105
      Average (%) 103 92 100
      1:8 Range (%) 97-113 82-98 99-110
      Average (%) 104 89 104
      1:16 Range (%) 96-111 82-92 93-107
      Average (%) 101 87 100

      Kit components & Storage

      An unopened kit can be stored at 2-8°C for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

      Item Specifications Storage
      Micro ELISA Plate(Dismountable) 8 wells ×12 strips -20°C, 6 months
      Reference Standard 2 vials
      Concentrated Biotinylated Detection Ab (100×) 1 vial, 120 μL
      Concentrated HRP Conjugate (100×) 1 vial, 120 μL -20°C(shading light), 6 months
      Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
      Biotinylated Detection Ab Diluent 1 vial, 14 mL
      HRP Conjugate Diluent 1 vial, 14 mL
      Concentrated Wash Buffer (25×) 1 vial, 30 mL
      Substrate Reagent 1 vial, 10 mL 2-8°C(shading light)
      Stop Solution 1 vial, 10 mL 2-8°C
      Plate Sealer 5 pieces
      Product Description 1 copy
      Certificate of Analysis 1 copy

      Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
      The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

      Other supplies required

      • Microplate reader with 450 nm wavelength filter
      • High-precision transfer pipette, EP tubes and disposable pipette tips
      • Incubator capable of maintaining 37°C
      • Deionized or distilled water
      • Absorbent paper
      • Loading slot for Wash Buffer

      Assay Procedure

      elisa assay procedure 1

      1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

      elisa assay procedure 2

      2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

      elisa assay procedure 3

      3. Aspirate and wash the plate for 3 times

      elisa assay procedure 4

      4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

      elisa assay procedure 5

      5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

      elisa assay procedure 6

      6. Add 50μL Stop Solution

      elisa assay procedure 7

      7. Read the plate at 450nm immediately. Calculation of the results

      Citations

      1. Therapeutic Effects Of Anti-B7-H3 Antibody In An Ovalbumin-Induced Mouse Asthma Model.
        IF: 3.728
        Journal: Annals of Allergy Asthma & Immunology(2013)
        DOI: 10.1016/j.anai.2013.06.030
        PMID: 24054363
        Products: E-EL-M0048 E-EL-M0043 E-EL-M0047 
        Sample type: Bronchoalveolar Lavage Fluid
        Reactivity: Mouse
      2. Ghrelin Ameliorates Intestinal Barrier Dysfunction In Experimental Colitis By Inhibiting The Activation Of Nuclear Factor-Kappa B.
        IF: 2.466
        Journal: Biochemical & Biophysical Research Communications(2015)
        DOI: 10.1016/j.bbrc.2015.01.083
        PMID: 25634696
        Products: E-EL-M0049 E-EL-M0048 E-EL-M0037 E-EL-M0044 E-EL-M0046 E-EL-M0047 
        Sample type: Tissue homogenate
        Reactivity: Mouse
      3. Immunopathological Assessments Of Human Blastocystis Spp. In Experimentally Infected Immunocompetent And Immunosuppresed Mice.
        IF: 2.329
        Journal: Parasitology Research(2016)
        DOI: 10.1007/s00436-016-4951-3
        PMID: 26860840
        Products: E-EL-M0048 E-EL-M0049 E-EL-M0043 E-EL-M0046 E-EL-M0726 E-EL-M1040 
        Sample type: Serum,Tissue homogenate
        Reactivity: Mouse
      4. Sulfated Modification Promotes The Immunomodulatory Bioactivities Of Lyciumbarbarum Polysaccharides In Vitro.
        IF: 1.069
        Journal: International Journal of Clinical and Experimental Medicine(2015)
        PMID: 26884954
        Products: E-EL-M0042 E-EL-M0044 E-EL-M0048 E-EL-M0049 
        Sample type: Cell culture supernatant
        Reactivity: Mouse
      5. Immunogenicity Of The Recombinant HxuCBA Proteins Encoded By HxuCBA Gene Cluster Of Haemophilus Parasuis In Mice.
        IF: 2.415
        Journal: Gene(2016)
        DOI: 10.1016/j.gene.2016.07.001
        PMID: 27378742
        Products: E-EL-M0042 E-EL-M0043 E-EL-M0048 
        Sample type: Serum
        Reactivity: Mouse
      6. Structural Analysis And Immunoregulation Activity Comparison Of Five Polysaccharides From Angelica Sinensis.
        IF: 4.811
        Journal: Carbohydrate Polymers(2016)
        DOI: 10.1016/j.carbpol.2015.12.050
        PMID: 26876821
        Products: E-EL-M0049 E-EL-M0048 E-EL-M0042 E-EL-M0044 
        Sample type: Cell culture supernatant
        Reactivity: Mouse
      7. Treatment Of Allergic Rhinitis With CpG Oligodeoxynucleotides Alleviates The Lower Airway Outcomes Of Combined Allergic Rhinitis And Asthma Syndrome Via A Mechanism That Possibly Involves In TSLP.
        IF: 1.394
        Journal: Experimental Lung Research(2016)
        DOI: 10.1080/01902148.2016.1215571
        PMID: 27541375
        Products: E-EL-M0048 
        Sample type: Bronchoalveolar Lavage Fluid
        Reactivity: Mouse
      8. Oral Immunization With Recombinant Lactobacillus Acidophilus Expressing espA-Tir-M Confers Protection Against Enterohemorrhagic Escherichia Coli O157: H7 Challenge In Mice.
        IF: 4.076
        Journal: Frontiers in Microbiology(2017)
        DOI: 10.3389/fmicb.2017.00417
        PMID: 28360900
        Products: E-EL-M0048 E-EL-M0043 E-EL-M0046 
        Sample type: Serum
        Reactivity: Mouse
      9. Rheb1 Deletion In Myeloid Cells Aggravates OVA-Induced Allergic Inflammation In Mice.
        IF: 4.259
        Journal: Scientific Reports(2017)
        DOI: 10.1038/srep42655
        PMID: 28225024
        Products: E-EL-M0042 E-EL-M0043 E-EL-M0727 E-EL-M0048 E-EL-M3034 
        Sample type: Bronchoalveolar Lavage Fluid,Serum
        Reactivity: Mouse
      10. Regulatory role of NKG2D+ NK cells in intestinal lamina propria by secreting double-edged Th1 cytokines in ulcerative colitis.
        IF: 5.168
        Journal: Oncotarget(2017)
        DOI: 10.18632/oncotarget.22132
        PMID: 29228739
        Products: E-EL-M0049 E-EL-M0048 E-EL-M0043 E-EL-M0044 E-EL-M0046 E-EL-M0047 
        Sample type: Tissue homogenate
        Reactivity: Mouse
      11. Peroxisome Proliferator-Activated Receptor-Γ Agonist Pioglitazone Fails To Attenuate Renal Fibrosis Caused By Unilateral Ureteral Obstruction In Mice.
        IF: 0.964
        Journal: Journal of Huazhong University Of Science And Technology-Medical Sciences(2016)
        DOI: 10.1007/s11596-016-1539-1
        PMID: 26838738
        Products: E-EL-M3001 E-EL-M0048 E-EL-M0043 E-EL-M0047 E-EL-M0046 
        Sample type: Tissue homogenate
        Reactivity: Mouse
      12. Intranasal Immunization With Novel EspA-Tir-M Fusion Protein Induces Protective Immunity Against Enterohemorrhagic Escherichia coli O157: H7 Challenge In Mice.
        IF: 2.009
        Journal: Microbial Pathogenesis(2017)
        DOI: 10.1016/j.micpath.2017.01.062
        PMID: 28163157
        Products: E-EL-M0048 E-EL-M0043 E-EL-M0046 
        Sample type: Serum
        Reactivity: Mouse
      13. Components of heat-treated Helianthus annuus L. pectin inhibit tumor growth and promote immunity in a mouse CT26 tumor model.
        IF: 3.47
        Journal: Journal of Functional Foods(2018)
        DOI: 10.1016/j.jff.2018.07.001
        Products: E-EL-M0048 E-EL-M0726 
        Sample type: Tissue homogenate
        Reactivity: Mouse
      14. Dendritic cell-targeted recombinant Lactobacilli induce DC activation and elicit specific immune responses against G57 genotype of avian H9N2 influenza virus infection.
        IF: 2.524
        Journal: Veterinary Microbiology(2018)
        DOI: 10.1016/j.vetmic.2018.07.009
        PMID: 30173758
        Products: E-EL-M0048 
        Sample type: Serum,Cell culture supernatant
        Reactivity: Mouse
      15. Effects of Simulated Heat Wave and Ozone on High Fat Diet ApoE Deficient Mice.
        IF: 2.518
        Journal: Biomedical and Environmental Sciences(2018)
        DOI: 10.3967/bes2018.101
        PMID: 30423277
        Products: E-EL-M0049 E-EL-M0044 E-EL-M0077 E-EL-M0687 E-EL-M0048 E-EL-M2730 E-EL-M0400 E-EL-M0914 E-EL-0060 E-EL-M0419 E-EL-M0618 E-BC-K035-M E-BC-K020-M 
        Sample type: Plasma,Tissue homogenate
        Reactivity: Mouse
      16. Fusion of thymosin alpha 1 with mutant IgG1 CH3 prolongs half-life and enhances antitumor effects in vivo
        IF: 3.361
        Journal: International Immunopharmacology(2019)
        DOI: 10.1016/j.intimp.2019.05.047
        PMID: 31220695
        Products: E-EL-M0042 E-EL-M0048 
        Sample type: Serum
        Reactivity: Mouse
      17. Fusion of thymosin alpha 1 with mutant IgG1 CH3 prolongs half-life and enhances antitumor effects in vivo
        IF: 3.361
        Products: E-EL-M0042 E-EL-M0048 
        Sample type: Serum
        Reactivity: Mouse
      18. NanoRNP Overcomes Tumor Heterogeneity in Cancer Treatment
        IF: 12.279
        Journal: NANO LETTERS(2019)
        DOI: 10.1021/acs.nanolett.9b02501
        PMID: 31593471
        Products: E-EL-M0692 E-EL-M3034 E-EL-M0695 E-EL-M0044 E-EL-M0048 E-EL-M0049 
        Sample type: Serum
        Reactivity: Mouse
      19. Dual-Locking Nanoparticles Disrupt the PD-1/PD-L1 Pathway for Efficient Cancer Immunotherapy
        IF: 25.809
        Journal: Advanced materials(2019)
        DOI: 10.1002/adma.201905751
        PMID: 31709671
        Products: E-EL-0162 E-EL-M0048 E-EL-M0049 E-EL-M0042 E-EL-M0726 
        Sample type: Tissue homogenate
        Reactivity: Mouse
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