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Mouse IGF-1(Insulin-like Growth Factor 1) ELISA Kit

Citations(7)Uniprot : P05017

Cat.No.:E-EL-M3006
Reactivity: Mouse

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	15.63-1000 ng/mL
Sensitivity	9.38 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Mouse IGF-1 in samples. No significant cross-reactivity or interference between Mouse IGF-1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Mouse IGF-1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IGF-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IGF-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IGF-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IGF-1. You can calculate the concentration of Mouse IGF-1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IGF-1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IGF-1 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	41.54	117.97	459.78	45.45	123.54	450.44
Standard deviation	2.23	5.39	21.79	2.50	5.60	22.03
CV (%)	5.37	4.57	4.74	5.50	4.53	4.89

Recovery

The recovery of Mouse IGF-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	87-97	92
EDTA plasma (n=8)	89-100	97
Cell culture media (n=8)	86-100	92

Linearity

Samples were spiked with high concentrations of Mouse IGF-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P05017
Synonyms	IGF1, IGFI, IGF-I, IGF1A, IGF-IA, IGF-IB, MGF, Somatomedin C
Research Area	Cancer, Developmental biology, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

JOURNAL OF NUTRITIONAL BIOCHEMISTRY (2022) IF: 6.117
Maternal omega-3 fatty acid deficiency affects fetal thermogenic development and postnatal musculoskeletal growth in mice

DOI: 10.1016/j.jnutbio.2022.109218

Sample: Plasma
Cancers (2020) IF: 6.126
Retinol-Binding Protein 4 Accelerates Metastatic Spread and Increases Impairment of Blood Flow in Mouse Mammary Gland Tumors

DOI: 10.3390/cancers12030623

Sample: plasma
Oncotarget (2017) IF: 5.168
Emodin and rhein decrease levels of hypoxia-inducible factor-1α in human pancreatic cancer cells and attenuate cancer cachexia in athymic mice carrying these cells

DOI: 10.18632/oncotarget.21330

Sample: Tissue homogenate
BMC CANCER (2018) IF: 3.288
The Warburg effect in human pancreatic cancer cells triggers cachexia in athymic mice carrying the cancer cells

DOI: 10.1186/s12885-018-4271-3

Sample: Cell culture supernatant
Dose-Response (2020) IF: 2.438
Serum Proteins as New Biomarkers for Whole-Body Exposure to High- and Low-LET Ionizing Radiation:

DOI: 10.1177/1559325820914172

Sample: serum
PLACENTA (2015) IF: 2.71
The effect of maternal obesity on the expression and functionality of placental P-glycoprotein: Implications in the individualized transplacental digoxin treatment for fetal heart failure

DOI: 10.1016/j.placenta.2015.08.007

Sample: Plasma
Advances in Pharmacological and Pharmaceutical Sciences (2020)
Antihypernociceptive, Anxiolytic, and Antidepressant Properties of Aqueous and Ethanol Extracts of Dissotis thollonii Cogn. (Melastomataceae) in Mice

DOI: 10.1155/2020/8886894

PMID: 33015629
Sample: Serum,Tissue homogenate

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