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Mouse IL-12(Interleukin 12) ELISA Kit

  • Cat.No.:E-EL-M0726

  • Reactivity: Mouse

To Purchase E-EL-M0726

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse IL-12 in samples. No significant cross-reactivity or interference between Mouse IL-12 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse IL-12 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse IL-12. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse IL-12 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse IL-12, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse IL-12. You can calculate the concentration of Mouse IL-12 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse IL-12 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse IL-12 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 50.70 123.90 464.80 53.40 119.50 423.10
Standard deviation 2.70 5.60 15.30 3.30 5.10 14.40
CV (%) 5.33 4.52 3.29 6.18 4.27 3.40

Recovery

The recovery of Mouse IL-12 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 83-96 90
EDTA plasma (n=8) 88-102 94
Cell culture media (n=8) 87-97 92

Linearity

Samples were spiked with high concentrations of Mouse IL-12 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: P43432
SynonymsIL12, IL-12 p70, IL12 p70
Research AreaCancer

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Related Products

Elabscience® provides ancillary reagents required for ELISA kit developments.11 reagents in total, including reagents for coating, blocking, washing, improving the stability and reducing matrix interference etc.
Cat.No. Product Name Specifications
E-ELIR-001 ELISA Plate Coating Buffer(1×) 100mL, 1000mL
E-ELIR-002 ELISA Plate Coating Buffer(5×) 100mL, 1000mL
E-ELIR-003 ELISA Plate Blocking Buffer 100mL, 1000mL
E-ELIR-004 Wash Buffer for Sandwich-ELISA(25×) 100mL, 1000mL
E-ELIR-005 Wash Buffer for Competitive-ELISA(25×) 100mL, 1000mL
E-ELIR-006 Stop Solution 100mL, 1000mL
E-ELIR-007 HRP-conjugate Stabilizer 10mL, 100mL, 500mL
E-ELIR-008 HRP-conjugate Diluent 100mL, 1000mL
E-ELIR-009 Biotinylated Antibody Stabilizer 10mL, 100mL, 500mL
E-ELIR-0010 Biotinylated Antibody Diluent 10mL, 100mL, 500mL
E-ELIR-0011 Sample Diluent 10mL, 100mL, 500mL

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Advanced materials (2019) IF: 27.398
    Dual-Locking Nanoparticles Disrupt the PD-1/PD-L1 Pathway for Efficient Cancer Immunotherapy

    DOI: 10.1002/adma.201905751

    PMID: 31709671

    Sample: Tissue homogenate
  2. Advanced functional materials (2020) IF: 16.836
    Quantitative Photoacoustic Diagnosis and Precise Treatment of Inflammation In Vivo Using Activatable Theranostic Nanoprobe

    DOI: 10.1002/adfm.202001771

    PMID: 99999999

    Sample: Serum
  3. Advanced Functional Materials (2019) IF: 16.836
    NIR/ROS-Responsive Black Phosphorus QD Vesicles as Immunoadjuvant Carrier for Specific Cancer Photodynamic Immunotherapy

    DOI: 10.1002/adfm.201905758

    PMID: 99999999

    Sample: Serum
  4. Chem. Sci. (2020) IF: 9.346
    A tumor acidity activatable and Ca2+-assisted immuno-nanoagent enhances breast cancer therapy and suppresses cancer recurrence

    DOI: 10.1039/d0sc00293c

    PMID: 99999999

    Sample: Serum
  5. Theranostics (2020) IF: 8.579
    Magnetic Targeted Near- Infrared II PA/MR Imaging-GuidedPhotothermal Therapy to Trigger Cancer Immunotherapy

    DOI: 10.7150/thno.43604

    PMID: 99999999

    Sample: Serum
  6. Oxidative medicine and cellular longevity (2019) IF: 5.076
    Targeting IL-17A Improves the Dysmotility of the Small Intestine and Alleviates the Injury of the Interstitial Cells of Cajal during Sepsis

    DOI: 10.1155/2019/1475729

    PMID: 31531179

    Sample: Tissue homogenate
  7. International Journal of Pharmaceutics (2020) IF: 4.845
    Targeted delivery of zoledronic acid through the sialic acid - Siglec axis for killing and reversal of M2 phenotypic tumor-associated macrophages - A promising cancer immunotherapy

    DOI: 10.1016/j.ijpharm.2020.119929

    PMID: 33010395

    Sample: Serum,Tissue homogenate
  8. Journal of Cellular and Molecular Medicine (2020) IF: 4.486
    Defibrotide inhibits donor leucocyte-endothelial interactions and protects against acute graft-versus-host disease

    DOI: 10.1111/jcmm.15434

    PMID: 32519822

    Sample: Plasma
  9. international journal of pharmaceutics (2020) IF: 4.845
    Targeted delivery of zoledronic acid thrpugh the sialic acid siglec axis for killing and reversal of M2 phenotypic tumor-associated macrophages-A promising cancer immunotherapy

    DOI: http://doi.org/10.1016/j.ijpharm.2020.119929

    PMID: 99999999

    Sample: Serum,Cell lysate,Tissue homogenate
  10. Microbial Cell Factories (2020) IF: 4.187
    A probiotic treatment increases the immune response induced by the nasal delivery of spore-adsorbed TTFC

    DOI: 10.1186/s12934-020-01308-1

    PMID: 32075660

    Sample: Cell culture supernatant
  11. Nutrients (2020) IF: 4.546
    Fermented Morinda citrifolia (Noni) Alleviates DNCB-Induced Atopic Dermatitis in NC/Nga Mice through Modulating Immune Balance and Skin Barrier Function

    DOI: 10.3390/nu12010249

    PMID: 31963703

    Sample: Serum,Cell culture supernatant
  12. Scientific Reports (2017) IF: 4.259
    Glycyrrhiza Uralensis Water Extract Enhances Dendritic Cell Maturation And Antitumor Efficacy Of HPV Dendritic Cell-Based Vaccine.

    DOI: 10.1038/srep43796

    PMID: 28272545

    Sample: Cell culture supernatant
  13. Scientific reports (2020) IF: 3.998
    Latexin deficiency in mice up-regulates inflammation and aggravates colitis through HECTD1/Rps3/NF-κB pathway

    DOI: 10.1038/s41598-020-66789-x

    PMID: 32555320

    Sample: Serum
  14. Biomedicine & Pharmacotherapy (2019) IF: 3.457
    Effects of iron chelating agent on Schistosoma mansoni infected murine model

    DOI: 10.1016/j.biopha.2018.10.036

    PMID: 30391706

    Sample: Serum,Tissue homogenate
  15. Journal of Functional Foods (2018) IF: 3.47
    Components of heat-treated Helianthus annuus L. pectin inhibit tumor growth and promote immunity in a mouse CT26 tumor model.

    DOI: 10.1016/j.jff.2018.07.001

    Sample: Tissue homogenate
  16. Food & Function (2017) IF: 3.247
    Purification, Characterization And Bioactivities Of Polysaccharides From Pleurotus Ferulae.

    DOI: 10.1039/c7fo00227k

    PMID: 28436508

    Sample: Cell culture supernatant
  17. Microbial Pathogenesis (2020) IF: 2.914
    Protective immunity induced by a DNA vaccine cocktail expressing TgSAG1, TgROP2, and the genetic adjuvant HBsAg against Toxoplasma gondii infection

    DOI: 10.1016/j.micpath.2020.104441

    PMID: 32781100

    Sample: Serum,Cell culture supernatant
  18. PeerJ (2020) IF: 2.379
    The immunostimulatory activity of polysaccharides from Glycyrrhiza uralensis

    DOI: 10.7717/peerj.8294

    PMID: 32030319

    Sample: Tissue homogenate
  19. Biochemical and biophysical research communications (2019) IF: 2.559
    Eosinophils attenuate arthritis by inducing M2 macrophage polarization via inhibiting the IkB/P38 MAPK signaling pathway

    DOI: 10.1016/j.bbrc.2018.12.010

    PMID: 30528734

    Sample: Tissue homogenate,Cell culture supernatant
  20. Parasitology Research (2016) IF: 2.329
    Immunopathological Assessments Of Human Blastocystis Spp. In Experimentally Infected Immunocompetent And Immunosuppresed Mice.

    DOI: 10.1007/s00436-016-4951-3

    PMID: 26860840

    Sample: Serum,Tissue homogenate
  21. Journal of Food Biochemistry (2019) IF: 1.662
    Immunomodulatory effect of papaya (Carica papaya) pulp and seed extracts as a potential natural treatment for bacterial stress

    DOI: 10.1111/jfbc.13050

    PMID: 31571245

    Sample: Serum
  22. Aesthetic plastic surgery (2019) IF: 1.484
    M1/M2 Macrophages Play Different Roles in Adipogenic Differentiation of PDGFRα+ Preadipocytes In Vitro

    DOI: 10.1007/s00266-018-1294-8

    PMID: 30552469

    Sample: Cell culture supernatant
  23. Functional Foods in Health and Disease (2018)
    Immunological effects of Kefir produced from Kefir grains versus starter cultures when fed to mice

    DOI: 10.31989/ffhd.v8i8.533

    PMID: 99999999

    Sample: Tissue homogenate
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