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Mouse INS(Insulin) ELISA Kit

Citations(11)Uniprot : P01325,P01326

Cat.No.:E-EL-M2614
Reactivity: Mouse

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T
Assay time	3.5h
Detection range	0.31-20 ng/mL
Sensitivity	0.19 ng/mL
Sample type &Sample volume	cell culture supernatant and tissue homogenate; 100μL
Specificity	This kit recognizes Mouse INS in samples. No significant cross-reactivity or interference between Mouse INS and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Mouse INS concentrations in cell culture supernatant and tissue homogenate.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse INS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse INS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse INS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse INS. You can calculate the concentration of Mouse INS in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse INS were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse INS were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	1.00	2.30	8.50	1.00	2.30	8.70
Standard deviation	0.10	0.10	0.40	0.10	0.10	0.30
CV (%)	10.00	4.35	4.71	10.00	4.35	3.45

Recovery

The recovery of Mouse INS spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Cell culture media (n=8)	95-110	101

Linearity

Samples were spiked with high concentrations of Mouse INS and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P01325 P01326
Synonyms	IDDM2, ILPR, IRDN, MODY10
Research Area	Cancer

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Cell Death & Disease (2021) IF: 8.4689
Inhibition of Notch activity promotes pancreatic cytokeratin 5-positive cell differentiation to beta cells and improves glucose homeostasis following acute pancreatitis

DOI: 10.1038/s41419-021-04160-2

PMID: 34556631
Sample: Krt5+ cell
FOOD RESEARCH INTERNATIONAL (2022) IF: 7.425
Effects of distinct n-6 to n-3 polyunsaturated fatty acid ratios on insulin resistant and AD-like phenotypes in high-fat diets-fed APP/PS1 mice

DOI: 10.1016/j.foodres.2022.112207

Sample: serum,cortical
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2020) IF: 4.192
Modulation of Gut Microbiota by Fucoxanthin During Alleviation of Obesity in High-Fat Diet-Fed Mice

DOI: 10.1021/acs.jafc.0c01467

PMID: 32309947
Sample: Serum,Tissue homogenate
PHARMACOLOGICAL RESEARCH (2018) IF: 4.897
Baicalin regulates SirT1/STAT3 pathway and restrains excessive hepatic glucose production

DOI: 10.1016/j.phrs.2018.08.018

PMID: 30144531
Sample: Cell culture supernatant
EXPERIMENTAL CELL RESEARCH (2019) IF: 3.329
The protective role of the MKP-5-JNK/P38 pathway in glucolipotoxicity-induced islet β-cell dysfunction and apoptosis

DOI: 10.1016/j.yexcr.2019.06.012

Sample: MIN6 cells
Evidence-based Complementary and Alternative Medicine (2021) IF: 2.63
Huang-Pu-Tong-Qiao Formula Ameliorates the Hippocampus Apoptosis in Diabetic Cognitive Dysfunction Mice by Activating CREB/BDNF/TrkB Signaling Pathway

DOI: 10.1155/2021/5514175

PMID: 34211563
Sample: hippocampu
EXPERIMENTAL BIOLOGY AND MEDICINE (2021) IF: 2.691
Sigma receptor knockdown augments dysfunction and apoptosis of beta cells induced by palmitate:

DOI: 10.1177/1535370221997780

Sample: Cell culture supernatant
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2018) IF: 2.8
In type 2 diabetes induced by cigarette smoking, activation of p38 MAPK is involved in pancreatic β-cell apoptosis

DOI: 10.1007/s11356-018-1337-3

Sample: MIN6 cells
Molecular Medicine Reports (2022) IF: 2.952
Sigma‑1 receptor overexpression promotes proliferation and ameliorates cell apoptosis in β‑cells

DOI: 10.3892/mmr.2022.12686

PMID: 35302175
Sample: MIN6 cell
Medical Molecular Morphology (2019) IF: 1.383
The therapeutic effects of resveratrol on hepatic steatosis in high-fat diet-induced obese mice by improving oxidative stress, inflammation and lipid-related gene transcriptional expression

DOI: 10.1007/s00795-019-00216-7

PMID: 30673851
Sample: Serum,Tissue homogenate
Journal of Diabetes and Metabolic Disorders (2022)
Primaquine activates Keratin 7 to treat diabetes and its complications

DOI: 10.1007/s40200-022-01135-8

PMID: 36404863
Sample: liver tissue

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