Mouse MCP-1(Monocyte Chemotactic Protein 1) ELISA Kit

    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience

      Catalog number:E-EL-M0006

      Synonyms:CCL2, GDCF-2, HC11, HSMCR30, MCAF, MCP1, SCYA2, SMC-CF

      • 96T
      • 24T
      - +
      Price: $580

      Reactivity: Mouse

      Detection Range: 15.63~1000 pg/mL

      Sensitivity: 9.38 pg/mL

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request

      Intended use

      This ELISA kit applies to the in vitro quantitative determination of Mouse MCP-1 concentrations in serum, plasma and other biological fluids.

      Test principle

      This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse MCP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse MCP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse MCP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse MCP-1. You can calculate the concentration of Mouse MCP-1 in the samples by comparing the OD of the samples to the standard curve.

      Assay type Sandwich
      Format 96T
      Assay time 3.5h
      Reactivity Mouse
      Detection Method Colormetric
      Detection Range 15.63—1000 pg/mL
      Sensitivity 9.38 pg/mL
      Sample Volume Required Per Well 100μL
      Sample Type Serum, plasma and other biological fluids


      This kit recognizes Mouse MCP-1 in samples. No significant cross-reactivity or interference between Mouse MCP-1 and analogues was observed.

      Typical data

      As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

      O.D Average Corrected
      1000 2.46
      2.466 2.388
      500 1.646
      1.662 1.584
      250 0.966
      0.956 0.878
      125 0.428
      0.439 0.361
      62.5 0.298
      0.286 0.208
      31.25 0.188
      0.178 0.1
      15.63 0.121
      0.129 0.051
      0 0.073
      0.078 --


      Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse MCP-1 were tested 20 times on one plate, respectively.
      Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse MCP-1 were tested on 3 different plates, 20 replicates in each plate.

      Intra-assay Precision Inter-assay Precision
      Sample 1 2 3 1 2 3
      n 20 20 20 20 20 20
      Mean (pg/mL) 53.80 117.30 449.90 48.70 116.20 487.50
      Standard deviation 3.40 6.10 23.40 2.60 6.00 16.10
      C V (%) 6.32 5.20 5.20 5.34 5.16 3.30


      The recovery of Mouse MCP-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

      Sample Type Range (%) Average Recovery (%)
      Serum (n=5) 88-100 94
      EDTA plasma (n=5) 86-96 91
      Cell culture media (n=5) 89-105 96


      Samples were spiked with high concentrations of Mouse MCP-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

      Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
      1:2 Range (%) 98-112 87-99 95-110
      Average (%) 104 94 101
      1:4 Range (%) 100-116 81-93 96-111
      Average (%) 107 87 104
      1:8 Range (%) 101-115 85-98 94-105
      Average (%) 106 91 99
      1:16 Range (%) 99-112 82-95 90-103
      Average (%) 106 87 97

      Kit components & Storage

      An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

      Item Specifications Storage
      Micro ELISA Plate(Dismountable) 8 wells ×12 strips -20℃, 6 months
      Reference Standard 2 vials
      Concentrated Biotinylated Detection Ab (100×) 1 vial, 120 μL
      Concentrated HRP Conjugate (100×) 1 vial, 120 μL -20℃(shading light), 6 months
      Reference Standard & Sample Diluent 1 vial, 20 mL 4℃, 6 months
      Biotinylated Detection Ab Diluent 1 vial, 14 mL
      HRP Conjugate Diluent 1 vial, 14 mL
      Concentrated Wash Buffer (25×) 1 vial, 30 mL
      Substrate Reagent 1 vial, 10 mL 4℃(shading light)
      Stop Solution 1 vial, 10 mL 4℃
      Plate Sealer 5 pieces
      Product Description 1 copy
      Certificate of Analysis 1 copy

      Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
      The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

      Other supplies required

      • Microplate reader with 450 nm wavelength filter
      • High-precision transfer pipette, EP tubes and disposable pipette tips
      • Incubator capable of maintaining 37℃
      • Deionized or distilled water
      • Absorbent paper
      • Loading slot for Wash Buffer

      Assay Procedure

      elisa assay procedure 1

      1.Add 100 μL standard or sample to each well. Incubate for 90 min at 37℃.

      elisa assay procedure 2

      2.Remove the liquid.

      elisa assay procedure 3

      3.Add 100 μL Biotinylated Detection Ab. Incubate for 1 hour at 37℃. Aspirate and wash 3 times.

      elisa assay procedure 4

      4.Add 100 μL HRP Conjugate. Incubate for 30 min at 37℃. Aspirate and wash 5 times.

      elisa assay procedure 5

      5.Add 90 μL of Substrate Reagent. Incubate for 15 min at 37℃.

      elisa assay procedure 6

      6.Add 50 μL Stop Solution.

      elisa assay procedure 7

      7.Read at 450 nm immediately. Calculation of results.


      1. Publication: Zhang M, Zhao H T, Cai J, et al. Chronic administration of mitochondrion-targeted peptide SS-31 prevents atherosclerotic development in ApoE knockout mice fed Western diet[J]. PloS one, 2017.
        Sample Type: Serum
      2. Publication: Zhang Y Q, Wang C, Guo Q Y, et al. Molecular Mechanisms Of the Analgesic Action Of Wu-tou Decoction On Neuropathic Pain In Mice Revealed Using Microarray And Network Analysis[J]. Acta Pharmacologica Sinica, 2017.
        Sample Type: Tissue homogenate
      3. Publication: Wang C, Kong X Y, Zhu C Y, et al. Wu-tou Decoction Attenuates Neuropathic Pain Via Suppressing Spinal Astrocytic IL-1R1/TRAF6/JNK Signaling[J]. Oncotarget, 2017, 8(54): 92864-92879.
        Sample Type: Tissue homogenate
      4. Publication: Cang D W, Guo K J, Zhao F C. Dendritic Cells Enhance UHMWPE Wear Particle-Induced Osteoclast Differentiation Of Macrophages[J]. Journal of Biomedical Materials Research Part A, 2015, 103(10): 3349-3354.
        Sample Type: Culture supernatant
      5. Publication: Zhang Y, Wang J, Zhou Q D, et al. Peroxisome Proliferator-Activated Receptor-Γ Agonist Pioglitazone Fails To Attenuate Renal Fibrosis Caused By Unilateral Ureteral Obstruction In Mice[J]. Journal of Huazhong University Of Science And Technology-Medical Sciences, 2016, 36(1): 41-47.
        Sample Type: Tissue lysates
      6. Publication: Wu D D, Gao B, Li M L, et al. Hydrogen Sulfide Mitigates Kidney Injury In High Fat Diet-Induced Obese Mice[J]. Oxidative Medicine And Cellular Longevity, 2016.
        Sample Type: Plasma
      7. Publication: Cheng X Y, Li Y Y, Huang C, et al. AMP-Activated Protein Kinase Reduces Inflammatory Responses And Cellular Senescence In Pulmonary Emphysema[J]. Oncotarget, 2017, 8(14): 22513-22523.
        Sample Type: Bronchoalveolar lavage fluid
      8. Publication: Xue F, Nie X, Shi J, et al. Quercetin Inhibits LPS-Induced Inflammation and ox-LDL-Induced Lipid Deposition[J]. Front Pharmacol, 2017, 8(e0130898):40.
        Sample Type: Cell
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