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Mouse RANTES(Regulated On Activation, Normal T-Cell Expressed and Secreted) ELISA Kit

Citations(5)Uniprot : P30882

Cat.No.:E-EL-M0009
Reactivity: Mouse

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	31.25-2000 pg/mL
Sensitivity	18.75 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Mouse RANTES in samples. No significant cross-reactivity or interference between Mouse RANTES and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Mouse RANTES concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse RANTES. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse RANTES and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse RANTES, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse RANTES. You can calculate the concentration of Mouse RANTES in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse RANTES were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse RANTES were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	100.20	288.20	866.90	94.20	312.30	800.90
Standard deviation	6.80	16.10	32.90	4.90	15.60	29.60
CV (%)	6.79	5.59	3.80	5.20	5.00	3.70

Recovery

The recovery of Mouse RANTES spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	84-99	91
EDTA plasma (n=8)	87-100	94
Cell culture media (n=8)	93-106	100

Target Information

Database Links	SwissProt: P30882
Synonyms	CCL5, SCYA5, SISd, TCP228, D17S136E
Research Area	Cancer, Cardiovascular, Immunology

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Cell Reports (2023) IF: 9.9949
RNF138 inhibits late inflammatory gene transcription through degradation of SMARCC1 of the SWI/SNF complex.

DOI: 10.1016/j.celrep.2023.112097

Sample: serum,RAW264.7 cell
FEBS LETTERS (2022) IF: 4.124
The Microglial membrane receptor TREM2 mediates exosome secretion to promote phagocytosis of amyloid-β by microglia

DOI: 10.1002/1873-3468.14336

PMID: 35292963
Sample: Bv2 cell
CLINICAL AND EXPERIMENTAL IMMUNOLOGY (2021) IF: 4.33
The role of Dock2 on macrophage migration and functions during Citrobacter rodentium infection

DOI: 10.1111/cei.13590

PMID: 33662140
Sample: Colon tissue
NEUROPHARMACOLOGY (2020) IF: 4.431
Seizure-induced neuroinflammation contributes to ectopic neurogenesis and aggressive behavior in pilocarpine-induced status epilepticus mice

DOI: 10.1016/j.neuropharm.2020.108044

PMID: 32179291
Sample: Cell lysate
Molecular Medicine Reports (2017) IF: 1.692
Protective effects of marrubiin improve endometriosis through suppression of the expression of RANTES

DOI: 10.3892/mmr.2017.6969

Sample: endometriotic cell-Xenograft

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