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Mouse SOD1(Superoxide Dismutase 1, Soluble) ELISA Kit

Citations(13)Uniprot : P08228

Cat.No.:E-EL-M2398
Reactivity: Mouse

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96T
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Product Details

Properties

Assay type	Competitive-ELISA
Format	96T/48T
Assay time	2.0h
Detection range	0.16-10 ng/mL
Sensitivity	0.10 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 50μL
Specificity	This kit recognizes SOD1 in samples. No significant cross-reactivity or interference between SOD1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of SOD1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with SOD1. During the reaction, SOD1 in the sample or standard competes with a fixed amount of SOD1 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to SOD1. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SOD1 in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8℃
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level SOD1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level SOD1 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.50	1.50	3.60	0.50	1.40	3.30
Standard deviation	0.03	0.08	0.19	0.03	0.07	0.11
CV (%)	6.00	5.33	5.28	6.00	5.00	3.33

Recovery

The recovery of SOD1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	93-106	100
EDTA plasma(n=8)	89-101	95
Cell culture media(n=8)	90-101	96

Linearity

Samples were spiked with high concentrations of Mouse SOD1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P08228
Synonyms	ALS, ALS1, IPOA, Superoxide dismutase [Cu-Zn]
Research Area	Cancer, Cell Biology, Metabolism, Signal transduction

Assay Procedures

	1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
	2. Aspirate and wash the plate for 3 times
	3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	5. Add 50μL Stop Solution
	6. Read the plate at 450nm immediately. Calculation of the results

Citations

INFLAMMATION (2022) IF: 4.092
TDAG51-Deficiency Podocytes are Protected from High-Glucose-Induced Damage Through Nrf2 Activation via the AKT–GSK-3β Pathway

DOI: 10.1007/s10753-022-01638-9

Sample: podocytes
NEUROCHEMISTRY INTERNATIONAL (2022) IF: 4.297
Calcium channel antagonists interfere with the mechanism of action of elastin-derived peptide VGVAPG in mouse cortical astrocytes in vitro

DOI: 10.1016/j.neuint.2022.105405

PMID: 35934159
Sample: astrocyte cell
BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
Endothelial progenitor cell–derived exosomes ameliorate endothelial dysfunction in a mouse model of diabetes

DOI: 10.1016/j.biopha.2020.110756

Sample: Serum
Frontiers in Physiology (2022) IF: 4.755
Abscopal effects of thoracic X-ray radiation on spermatogenesis in mice

DOI: 10.3389/fphys.2022.984429

PMID: 36091371
Sample: lung,serum,testis
PROCESS BIOCHEMISTRY (2022) IF: 4.885
Optimization of ultrasound-assisted extraction, structural, functional, and antioxidant properties of Glycyrrhiza uralensis seed protein

DOI: 10.1016/j.procbio.2022.11.001

Sample: serum,liver
NEUROTOXICITY RESEARCH (2019) IF: 3.311
The Elastin-Derived Peptide VGVAPG Does Not Activate the Inflammatory Process in Mouse Cortical Astrocytes In Vitro

DOI: 10.1007/s12640-019-00114-x

Sample: mouse astrocyte cell
PHARMACEUTICAL BIOLOGY (2023) IF: 3.889
Antihyperglycemic effects of Lysiphyllum strychnifolium leaf extract in vitro and in vivo

DOI: 10.1080/13880209.2022.2160771

Sample: muscle
Molecular Medicine Reports (2020) IF: 2.1
Coenzyme Q10 alleviates sevoflurane‑induced neuroinflammation by regulating the levels of apolipoprotein E and phosphorylated tau protein in mouse hippocampal neurons

DOI: 10.3892/mmr.2020.11131

Sample: Tissue homogenate
ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY (2019) IF: 2.11
The effects of spinosad on antioxidant system and cognitive performance of mice

DOI: 10.1080/13813455.2019.1623264

Sample: hippocampus,prefrontal cortex tissue
Experimental and Therapeutic Medicine (2021) IF: 2.447
Combination of endothelial progenitor cells and BB‑94 significantly alleviates brain damage in a mouse model of diabetic ischemic stroke

DOI: 10.3892/etm.2021.10221

Sample: Cell culture supernatant
MICROBIAL PATHOGENESIS (2019) IF: 2.581
Metabolites of stable fly reduce diarrhea in mice by modulating the immune system, antioxidants, and composition of gut microbiota

DOI: 10.1016/j.micpath.2019.103557

Sample: small intestinal tissues
HUMAN & EXPERIMENTAL TOXICOLOGY (2021) IF: 2.903
Apigenin alleviates methotrexate-induced liver and kidney injury in mice:

DOI: 10.1177/09603271211009964

Sample: Whole Blood,Tissue homogenate
Molecular Medicine Reports (2018) IF: 1.922
Embelin inhibits abdominal aortic aneurysm through decreasing IL‑6‑induced STAT3 and NF‑κB inactivation

DOI: 10.3892/mmr.2018.9221

Sample: Aortic tissues

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