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Mouse TGF-β1(Transforming Growth Factor Beta 1) ELISA Kit

Citations(36)

  • Cat.No.:E-EL-M0051

  • Reactivity: Mouse
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  • 96T
  • 24T
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T
Assay time 3.5h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume Serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse TGF-β1 in samples. No significant cross-reactivity or interference between Mouse TGF-β1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse TGF-β1 concentrations in Serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TGF-β1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TGF-β1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TGF-β1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TGF-β1. You can calculate the concentration of Mouse TGF-β1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse TGF-β1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse TGF-β1 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 95.20 254.00 667.10 95.80 270.00 634.70
Standard deviation 5.10 11.40 28.70 5.70 11.10 31.10
CV (%) 5.36 4.49 4.30 5.95 4.11 4.90

Recovery

The recovery of Mouse TGF-β1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-109 101
EDTA plasma (n=8) 93-110 101
Cell culture media (n=8) 93-107 99

Target Information

SynonymsTGFB1, CED, DPD1, LAP, TGFB, TGFbeta, transforming growth factor beta 1
Research AreaCancer,Cardiovascular,Metabolism,Neuroscience,Stem cells

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. ACS Applied Materials & Interfaces (2022) IF: 10.383
    Inflammation-Regulated Nanodrug Sensitizes Hepatocellular Carcinoma to Checkpoint Blockade Therapy by Reprogramming the Tumor Microenvironment

    DOI: 10.1021/acsami.2c14448

    PMID: 36314479

    		 Sample: Hepa 1-6 cells-xenograft
  2. ACS Applied Materials & Interfaces (2022) IF: 10.383
    In Situ Growth of Tunable Gold Nanoparticles by Titania Nanotubes Templated Electrodeposition for Improving Osteogenesis through Modulating Macrophages Polarization

    DOI: 10.1021/acsami.2c13976
  3. JOURNAL OF CONTROLLED RELEASE (2021) IF: 9.776
    Simultaneous blockage of contextual TGF-β by cyto-pharmaceuticals to suppress breast cancer metastasis

    DOI: 10.1016/j.jconrel.2021.06.012

    PMID: 34119557

    		 Sample: lung
  4. Theranostics (2019) IF: 8.0630
    Temporal inhibition of mouse mammary gland cancer metastasis by CORM-A1 and DETA/NO combination therapy

    DOI: 10.7150/thno.31461
    Sample: mammary gland tumor tissue,plasma
  5. CELL PROLIFERATION (2022) IF: 8.7550
    Tetrahedral framework nucleic acids promote diabetic wound healing via the Wnt signalling pathway

    DOI: 10.1111/cpr.13316

    PMID: 35869570

    		 Sample: skin samples
  6. Frontiers in Immunology (2022) IF: 8.786
    Gut Microflora Modulates Th17/Treg Cell Differentiation in Experimental Autoimmune Prostatitis via the Short-Chain Fatty Acid Propionate

    DOI: 10.3389/fimmu.2022.915218

    PMID: 35860242

    		 Sample: serum,prostate tissue
  7. Frontiers in Immunology (2022) IF: 8.786
    Bifidobacterium breve intervention combined with environmental enrichment alleviates cognitive impairment by regulating the gut microbiota and microbial metabolites in Alzheimer's disease mice.

    DOI: 10.3389/fimmu.2022.1013664
    Sample: Hippocampal tissue
  8. CHEMOSPHERE (2020) IF: 7.086
    Interleukin 17A deficiency alleviates fluoride-induced testicular injury by inhibiting the immune response and apoptosis

    DOI: 10.1016/j.chemosphere.2020.128178
    Sample: interstitial fluid
  9. FOOD CHEMISTRY (2021) IF: 7.514
    A new method to reduce allergenicity by improving the functional properties of soybean 7S protein through covalent modification with polyphenols

    DOI: 10.1016/j.foodchem.2021.131589
    Sample: Spleen cells
  10. Journal of Materials Chemistry B (2022) IF: 7.571
    Mesoporous hollow Fe3O4 nanoparticles regulate the behavior of neuro-associated cells through induction of macrophages polarization in an alternating magnetic field

    DOI: 10.1039/D2TB00527A
    Sample: RAw 264.7 cell
  11. Cancers (2020) IF: 6.126
    Retinol-Binding Protein 4 Accelerates Metastatic Spread and Increases Impairment of Blood Flow in Mouse Mammary Gland Tumors

    DOI: 10.3390/cancers12030623
    Sample: 4T1 cell-xenograft
  12. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY (2020) IF: 6.291
    Silica particles disorganize the polarization of pulmonary macrophages in mice

    DOI: 10.1016/j.ecoenv.2020.110364

    PMID: 32114243

    		 Sample: Tissue homogenate
  13. Oxidative Medicine and Cellular Longevity (2022) IF: 6.543
    DNA Methyltransferase 3b Accelerates the Process of Atherosclerosis

    DOI: 10.1155/2022/5249367

    PMID: 35422896

    		 Sample: plasma
  14. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2020) IF: 5.162
    Insight into delivery of dermal fibroblast by non-biodegradable bacterial nanocellulose composite hydrogel on wound healing

    DOI: 10.1016/j.ijbiomac.2020.05.011
    Sample: skin
  15. Stem Cells International (2022) IF: 5.443
    Mesenchymal Stem Cell-Secreted TGF-β1 Restores Treg/Th17 Skewing Induced by Lipopolysaccharide and Hypoxia Challenge via miR-155 Suppression

    DOI: 10.1155/2022/5522828
    Sample: CD4+ T cell
  16. INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 5.714
    JMJD3/H3K27me3 epigenetic modification regulates Th17/Treg cell differentiation in ulcerative colitis

    DOI: 10.1016/j.intimp.2022.109000

    PMID: 35777266

    		 Sample: colon tissue
  17. Frontiers in Pharmacology (2022) IF: 5.811
    The Effect of Shionone on Sepsis-Induced Acute Lung Injury by the ECM1/STAT5/NF-κB Pathway

    DOI: 10.3389/fphar.2021.764247
    Sample: serum,RAW264.7 cell
  18. Drug Design Development and Therapy (2022) IF: 4.162
    Didymin Ameliorates Liver Fibrosis by Alleviating Endoplasmic Reticulum Stress and Glycerophospholipid Metabolism: Based on Transcriptomics and Metabolomics

    DOI: 10.2147/DDDT.S351092

    PMID: 35698653

    		 Sample: liver
  19. Nutrients (2019) IF: 4.171
    Red Quinoa Bran Extracts Protects against Carbon Tetrachloride-Induced Liver Injury and Fibrosis in Mice via Activation of Antioxidative Enzyme Systems and Blocking TGF-β1 Pathway

    DOI: 10.3390/nu11020395

    PMID: 30781895

    		 Sample: Tissue homogenate
  20. Orphanet Journal of Rare Diseases (2022) IF: 4.303
    Immunological consequences of compromised ocular immune privilege accelerate retinal degeneration in retinitis pigmentosa

    DOI: 10.1186/s13023-022-02528-x

    PMID: 36253797

    		 Sample: serum
  21. TOXICOLOGY AND APPLIED PHARMACOLOGY (2023) IF: 4.46
    Dihydrokaempferol attenuates CCl4-induced hepatic fibrosis by inhibiting PARP-1 to affect multiple downstream pathways and cytokines

    DOI: 10.1016/j.taap.2023.116438
    Sample: serum
  22. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2020) IF: 4.486
    Mechanistic insights into the effects of SREBP1c on hepatic stellate cell and liver fibrosis

    DOI: 10.1111/jcmm.15614

    PMID: 32678475

    		 Sample: Serum
  23. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (2020) IF: 4.486
    Defibrotide inhibits donor leucocyte‐endothelial interactions and protects against acute graft‐versus‐host disease

    DOI: 10.1111/jcmm.15434
    Sample: Plasma
  24. Frontiers in Physiology (2022) IF: 4.755
    Abscopal effects of thoracic X-ray radiation on spermatogenesis in mice

    DOI: 10.3389/fphys.2022.984429

    PMID: 36091371

    		 Sample: lung,serum,testis
  25. INTERNATIONAL JOURNAL OF PHARMACEUTICS (2020) IF: 4.845
    Targeted delivery of zoledronic acid through the sialic acid - Siglec axis for killing and reversal of M2 phenotypic tumor-associated macrophages – A promising cancer immunotherapy

    DOI: 10.1016/j.ijpharm.2020.119929
    Sample: Serum,Tissue homogenate
  26. INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 4.932
    IL-25 improves diabetic wound healing through stimulating M2 macrophage polarization and fibroblast activation

    DOI: 10.1016/j.intimp.2022.108605

    PMID: 35149293

    		 Sample: Macrophage
  27. INTERNATIONAL IMMUNOPHARMACOLOGY (2019) IF: 4.932
    Arsenic trioxide improves Treg and Th17 balance by modulating STAT3 in treatment-naïve rheumatoid arthritis patients

    DOI: 10.1016/j.intimp.2019.05.001
    Sample: serum
  28. EXPERIMENTAL BIOLOGY AND MEDICINE (2020) IF: 3.139
    Exosomes derived from synovial fibroblasts under hypoxia aggravate rheumatoid arthritis by regulating Treg/Th17 balance:

    DOI: 10.1177/1535370220934736
    Sample: Cell culture medium
  29. DIGESTIVE DISEASES AND SCIENCES (2019) IF: 3.199
    Role of Interleukin-17 in Pathogenesis of Intestinal Fibrosis in Mice

    DOI: 10.1007/s10620-019-05969-w
    Sample: blood
  30. American Journal of Translational Research (2020) IF: 3.375
    Effects of catalpol on asthma by airway remodeling via inhibiting TGF-β1 and EGF in ovalbumin-induced asthmatic mice

    PMID: 32774762

    		 Sample: serum,BALF
  31. Biomedical Materials (2021) IF: 3.715
    The osteoimmunomodulatory effect of nanostructured TiF x /TiO x coating on osteogenesis induction

    DOI: 10.1088/1748-605X/ac0863
    Sample: RAW264.7 cells
  32. CIRCULATION JOURNAL (2020) IF: 2.54
    Sustaining Circulating Regulatory T Cell Subset Contributes to the Therapeutic Effect of Paroxetine on Mice With Diabetic Cardiomyopathy

    DOI: 10.1253/circj.CJ-19-1182

    PMID: 32741881

    		 Sample: Serum
  33. MICROBIAL PATHOGENESIS (2020) IF: 2.914
    Exposure time determines the protective effect of Trichinella spiralis on experimental colitis

    DOI: 10.1016/j.micpath.2020.104263

    PMID: 32442663

    		 Sample: Serum
  34. PeerJ (2022) IF: 2.984
    Quercetin prevents chronic kidney disease on mesangial cells model by regulating inflammation, oxidative stress, and TGF-β1/SMADs pathway

    DOI: 10.7717/peerj.13257

    PMID: 35673387

    		 Sample: SV40 MES 13 cell
  35. AESTHETIC PLASTIC SURGERY (2018) IF: 1.484
    Preadipocytes In Vitro

    DOI: 10.1007/s00266-018-1294-8
    Sample: Cell culture supernatant
  36. Journal of Ayurveda and Integrative Medicine (2017)
    Exploring molecular mechanism of bone-forming capacity of Eurycoma longifolia: Evidence of enhanced expression of bone-related biomarkers

    DOI: 10.1016/j.jaim.2017.04.005
    Sample: calvariae origin osteoblastic cell line subclone 4
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