Mouse TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
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    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience

      Catalog number:E-EL-M0049

      Synonyms:DIF, TNF-alpha, TNFA, TNFSF2,TNF,TNFa

      Size:
      • 96T
      • 24T
      Qty:
      - +
      Price: $495

      Reactivity: Mouse

      Detection Range: 31.25~2000 pg/mL

      Sensitivity: 18.75 pg/mL

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request

      Intended use

      This ELISA kit applies to the in vitro quantitative determination of Mouse TNF-α concentrations in Serum, plasma and other biological fluids.

      Test principle

      This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TNF-α . Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TNF-α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TNF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TNF-α. You can calculate the concentration of Mouse TNF-α in the samples by comparing the OD of the samples to the standard curve.

      Assay type Sandwich
      Format 96T
      Assay time 3.5h
      Reactivity Mouse
      Detection Method Colormetric
      Detection Range 31.25—2000 pg/mL
      Sensitivity 18.75 pg/mL
      Sample Volume Required Per Well 100μL
      Sample Type Serum, plasma and other biological fluids

      Specificity

      This kit recognizes Mouse TNF-α in samples. No significant cross-reactivity or interference between Mouse TNF-α and analogues was observed.

      Typical data

      As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

      Concentration
      (pg/mL)
      O.D Average Corrected
      2000 2.449
      2.491
      2.47 2.39
      1000 1.726
      1.752
      1.739 1.659
      500 0.993
      0.991
      0.992 0.912
      250 0.483
      0.509
      0.496 0.416
      125 0.3
      0.282
      0.291 0.211
      62.5 0.196
      0.168
      0.182 0.102
      31.25 0.128
      0.136
      0.132 0.052
      0 0.079
      0.081
      0.08 --

      Precision

      Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse TNF-α were tested 20 times on one plate, respectively.
      Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse TNF-α were tested on 3 different plates, 20 replicates in each plate.

      Intra-assay Precision Inter-assay Precision
      Sample 1 2 3 1 2 3
      n 20 20 20 20 20 20
      Mean (pg/mL) 92.50 285.40 722.10 85.50 297.30 763.30
      Standard deviation 6.00 16.60 33.90 4.50 11.90 41.20
      C V (%) 6.49 5.82 4.69 5.26 4.00 5.40

      Recovery

      The recovery of Mouse TNF-α spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

      Sample Type Range (%) Average Recovery (%)
      Serum (n=5) 94-111 101
      EDTA plasma (n=5) 96-110 102
      Cell culture media (n=5) 88-104 96

      Linearity

      Samples were spiked with high concentrations of Mouse TNF-α and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

      Serum (n=5) EDTA plasma (n=5) Cell culture media (n=5)
      1:2 Range (%) 95-106 89-101 88-102
      Average (%) 101 94 94
      1:4 Range (%) 95-111 81-91 91-105
      Average (%) 103 86 98
      1:8 Range (%) 96-112 84-97 91-106
      Average (%) 103 91 98
      1:16 Range (%) 95-112 85-96 95-106
      Average (%) 103 91 101

      Kit components & Storage

      An unopened kit can be stored at 2-8°C for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.

      Item Specifications Storage
      Micro ELISA Plate(Dismountable) 8 wells ×12 strips -20°C, 6 months
      Reference Standard 2 vials
      Concentrated Biotinylated Detection Ab (100×) 1 vial, 120 μL
      Concentrated HRP Conjugate (100×) 1 vial, 120 μL -20°C(shading light), 6 months
      Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
      Biotinylated Detection Ab Diluent 1 vial, 14 mL
      HRP Conjugate Diluent 1 vial, 14 mL
      Concentrated Wash Buffer (25×) 1 vial, 30 mL
      Substrate Reagent 1 vial, 10 mL 2-8°C(shading light)
      Stop Solution 1 vial, 10 mL 2-8°C
      Plate Sealer 5 pieces
      Product Description 1 copy
      Certificate of Analysis 1 copy

      Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.
      The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

      Other supplies required

      • Microplate reader with 450 nm wavelength filter
      • High-precision transfer pipette, EP tubes and disposable pipette tips
      • Incubator capable of maintaining 37°C
      • Deionized or distilled water
      • Absorbent paper
      • Loading slot for Wash Buffer

      Assay Procedure

      elisa assay procedure 1

      1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

      elisa assay procedure 2

      2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

      elisa assay procedure 3

      3. Aspirate and wash the plate for 3 times

      elisa assay procedure 4

      4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

      elisa assay procedure 5

      5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

      elisa assay procedure 6

      6. Add 50μL Stop Solution

      elisa assay procedure 7

      7. Read the plate at 450nm immediately. Calculation of the results

      Citations

      1. Ghrelin Ameliorates Intestinal Barrier Dysfunction In Experimental Colitis By Inhibiting The Activation Of Nuclear Factor-Kappa B.
        IF: 2.466
        Journal: Biochemical & Biophysical Research Communications(2015)
        DOI: 10.1016/j.bbrc.2015.01.083
        PMID: 25634696
        Products: E-EL-M0049 E-EL-M0048 E-EL-M0037 E-EL-M0044 E-EL-M0046 E-EL-M0047 
        Sample type: Tissue homogenate
        Reactivity: Mouse
      2. Immunopathological Assessments Of Human Blastocystis Spp. In Experimentally Infected Immunocompetent And Immunosuppresed Mice.
        IF: 2.329
        Journal: Parasitology Research(2016)
        DOI: 10.1007/s00436-016-4951-3
        PMID: 26860840
        Products: E-EL-M0048 E-EL-M0049 E-EL-M0043 E-EL-M0046 E-EL-M0726 E-EL-M1040 
        Sample type: Serum,Tissue homogenate
        Reactivity: Mouse
      3. The TIR/BB-Loop Mimetic AS-1 Attenuates Mechanical Stress-Induced Cardiac Fibroblast Activation And Paracrine Secretion Via Modulation Of Large Tumor Suppressor Kinase 1.
        IF: 5.476
        Journal: Biochimica Et Biophysica Acta-Molecular Basis of Disease(2016)
        DOI: 10.1016/j.bbadis.2016.03.002
        PMID: 26964796
        Products: E-EL-M0049 E-EL-M0037 
        Sample type: Cell culture supernatant
        Reactivity: Mouse
      4. A Comparative Study Of The Effects Upon LPS Induced Macrophage RAW264.7 Inflammation In Vitro Of The Lipids Of Hippocampus Trimaculatus Leach.
        IF: 1.076
        Journal: Journal of Oleo Science(2015)
        DOI: 10.5650/jos.ess15190
        PMID: 26582156
        Products: E-EL-M0049 E-EL-M0037 E-EL-M0044 
        Sample type: Cell culture supernatant
        Reactivity: Mouse
      5. PGC-1α Silencing Compounds The Perturbation Of Mitochondrial Function Caused By Mutant SOD1 In Skeletal Muscle Of ALS Mouse Model.
        IF: 4.504
        Journal: Frontiers in Aging Neuroscience(2015)
        DOI: 10.3389/fnagi.2015.00204
        PMID: 26539112
        Products: E-EL-M0049 E-EL-M0037 E-EL-M0044 E-EL-M0007 
        Sample type: Serum
        Reactivity: Mouse
      6. Sulfated Modification Promotes The Immunomodulatory Bioactivities Of Lyciumbarbarum Polysaccharides In Vitro.
        IF: 1.069
        Journal: International Journal of Clinical and Experimental Medicine(2015)
        PMID: 26884954
        Products: E-EL-M0042 E-EL-M0044 E-EL-M0048 E-EL-M0049 
        Sample type: Cell culture supernatant
        Reactivity: Mouse
      7. Inhibition Of Hepatocyte Apoptosis: An Important Mechanism Of Corn Peptides Attenuating Liver Injury Induced By Ethanol.
        IF: 3.226
        Journal: International Journal of Molecular Sciences(2015)
        DOI: 10.3390/ijms160922062
        PMID: 26378531
        Products: E-EL-M0049 E-EL-0162 
        Sample type: Serum,Tissue homogenate
        Reactivity: Mouse
      8. The Effect Of Maternal Obesity On The Expression And Functionality Of Placental P-Glycoprotein: Implications In The Individualized Transplacental Digoxin Treatment For Fetal Heart Failure.
        IF: 2.759
        Journal: Placenta(2015)
        DOI: 10.1016/j.placenta.2015.08.007
        PMID: 26311557
        Products: E-EL-M0037 E-EL-M3006 E-EL-M0049 
        Sample type: Plasma
        Reactivity: Mouse
      9. Capparis Spinosa Fruit Ethanol Extracts Exert Different Effects On The Maturation Of Dendritic Cells.
        IF: 2.861
        Journal: Molecules(2017)
        DOI: 10.3390/molecules22010097
        PMID: 28067853
        Products: E-EL-M2451 E-EL-M0046 E-EL-M0044 E-EL-M0037 E-EL-M0049 
        Sample type: Cell culture supernatant
        Reactivity: Mouse
      10. Effects Of Silver Nanoparticles In Combination With Antibiotics On The Resistant Bacteria Acinetobacter Baumannii.
        IF: 4.3
        Journal: International Journal of Nanomedicine(2016)
        DOI: 10.2147/IJN.S104166
        PMID: 27574420
        Products: E-EL-M0044 E-EL-M0049 
        Sample type: Plasma
        Reactivity: Mouse
      11. Structural Analysis And Immunoregulation Activity Comparison Of Five Polysaccharides From Angelica Sinensis.
        IF: 4.811
        Journal: Carbohydrate Polymers(2016)
        DOI: 10.1016/j.carbpol.2015.12.050
        PMID: 26876821
        Products: E-EL-M0049 E-EL-M0048 E-EL-M0042 E-EL-M0044 
        Sample type: Cell culture supernatant
        Reactivity: Mouse
      12. MiR-146a Modulates Macrophage Polarization By Inhibiting Notch1 Pathway In RAW264. 7 Macrophages.
        IF: 2.956
        Journal: International Immunopharmacology(2016)
        DOI: 10.1016/j.intimp.2016.01.009
        PMID: 26800097
        Products: E-EL-M0049 E-EL-M0046 
        Sample type: Cell culture supernatant
        Reactivity: Mouse
      13. Glycyrrhiza Uralensis Water Extract Enhances Dendritic Cell Maturation And Antitumor Efficacy Of HPV Dendritic Cell-Based Vaccine.
        IF: 4.259
        Journal: Scientific Reports(2017)
        DOI: 10.1038/srep43796
        PMID: 28272545
        Products: E-EL-M0037 E-EL-M0044 E-EL-M0726 E-EL-M0049 
        Sample type: Cell culture supernatant
        Reactivity: Mouse
      14. Repression Of Toll-Like Receptor-4 By microRNA-149-3p Is Associated With Smoking-Related COPD.
        IF: 3.157
        Journal: International Journal of Chronic Obstructive Pulmonary Disease(2017)
        DOI: 10.2147/COPD.S128031
        PMID: 28260877
        Products: E-EL-M0037 E-EL-M0049 
        Sample type: Cell culture supernatant
        Reactivity: Mouse
      15. Purification, Characterization And Bioactivities Of Polysaccharides From Pleurotus Ferulae.
        IF: 3.247
        Journal: Food & Function(2017)
        DOI: 10.1039/c7fo00227k
        PMID: 28436508
        Products: E-EL-M0726 E-EL-M0049 
        Sample type: Cell culture supernatant
        Reactivity: Mouse
      16. Antitumor And Immunomodulatory Effects Of Ginsenoside Rh2 And Its Octyl Ester Derivative In H22 Tumor-Bearing Mice.
        IF: 3.144
        Journal: Journal of Functional Foods(2017)
        DOI: 10.1016/j.jff.2017.03.013
        Products: E-EL-M0042 E-EL-M0049 
        Sample type: Serum
        Reactivity: Mouse
      17. Wogonin Attenuates Inflammation By Activating PPAR-γ In Alcoholic Liver Disease.
        IF: 2.956
        Journal: International Immunopharmacology(2017)
        DOI: 10.1016/j.intimp.2017.06.013
        PMID: 28646664
        Products: E-EL-M0049 E-EL-M0044 
        Sample type: Serum,Cell culture supernatant
        Reactivity: Mouse
      18. Effects Of Sepsis On The Metabolism Of Sphingomyelin And Cholesterol In Mice With Liver Dysfunction.
        IF: 1.261
        Journal: Experimental and Therapeutic Medicine(2017)
        DOI: 10.3892/etm.2017.5226
        PMID: 29285103
        Products: E-EL-M0037 E-EL-M0049 
        Sample type: Plasma
        Reactivity: Mouse
      19. Molecular Mechanisms Of the Analgesic Action Of Wu-tou Decoction On Neuropathic Pain In Mice Revealed Using Microarray And Network Analysis.
        IF: 3.223
        Journal: Acta Pharmacologica Sinica(2017)
        DOI: 10.1038/aps.2017.110
        PMID: 28816231
        Products: E-EL-M0037 E-EL-M0049 E-EL-M0018 E-EL-M3001 
        Sample type: Tissue homogenate
        Reactivity: Mouse
      20. Regulatory role of NKG2D+ NK cells in intestinal lamina propria by secreting double-edged Th1 cytokines in ulcerative colitis.
        IF: 5.168
        Journal: Oncotarget(2017)
        DOI: 10.18632/oncotarget.22132
        PMID: 29228739
        Products: E-EL-M0049 E-EL-M0048 E-EL-M0043 E-EL-M0044 E-EL-M0046 E-EL-M0047 
        Sample type: Tissue homogenate
        Reactivity: Mouse
      21. Wu-tou Decoction Attenuates Neuropathic Pain Via Suppressing Spinal Astrocytic IL-1R1/TRAF6/JNK Signaling.
        IF: 5.168
        Journal: Oncotarget(2017)
        DOI: 10.18632/oncotarget.21638
        PMID: 29190962
        Products: E-EL-M0049 E-EL-M0037 E-EL-M3001 E-EL-M0018 
        Sample type: Tissue homogenate
        Reactivity: Mouse
      22. Blocking The CD38/cADPR Pathway Plays A Double-edged Role In LPS Stimulated Microglia.
        IF: 3.277
        Journal: Neuroscience(2017)
        DOI: 10.1016/j.neuroscience.2017.08.010
        PMID: 28807785
        Products: E-EL-M0049 E-EL-M0044 E-EL-M0037 
        Sample type: Cell culture supernatant
        Reactivity: Mouse
      23. Dendritic Cells Enhance UHMWPE Wear Particle-Induced Osteoclast Differentiation Of Macrophages.
        IF: 3.076
        Journal: Journal of Biomedical Materials Research Part A(2015)
        DOI: 10.1002/jbm.a.35459
        PMID: 25808788
        Products: E-EL-M0049 E-EL-M3001 
        Sample type: Cell culture supernatant
        Reactivity: Mouse
      24. Hydrogen Sulfide Mitigates Kidney Injury In High Fat Diet-Induced Obese Mice.
        IF: 4.593
        Journal: Oxidative Medicine And Cellular Longevity(2016)
        DOI: 10.1155/2016/2715718
        PMID: 27413418
        Products: E-EL-M0049 E-EL-M0044 E-EL-M3001 E-EL-M0046 
        Sample type: Plasma
        Reactivity: Mouse
      25. Omentin-1 prevents inflammation-induced osteoporosis by downregulating the pro-inflammatory cytokines.
        IF: 9.326000000
        Journal: Bone research(2018)
        DOI: 10.1038/s41413-018-0012-0
        PMID: 29619269
        Products: E-EL-M0036 E-EL-M0037 E-EL-M0044 E-EL-M0049 E-EL-M3023 E-EL-M0857 E-EL-M1382 
        Reactivity: Mouse
      26. The Association Between Inflammation, The Microbiome and Urethane-induced Pulmonary Adenocarcinoma.
        IF: 1.39
        Journal: Oncology Letters(2018)
        DOI: 10.3892/ol.2018.8167
        PMID: 29725395
        Products: E-EL-M0838 E-EL-M0049 E-EL-M0037 E-EL-M0044 
        Reactivity: Mouse
      27. The protective effect of formononetin on cognitive impairment in streptozotocin (STZ)-induced diabetic mice.
        IF: 3.457
        Journal: Biomedicine & Pharmacotherapy(2018)
        DOI: 10.1016/j.biopha.2018.07.063
        PMID: 30119194
        Products: E-EL-M0037 E-EL-M0044 E-EL-M0049 
        Sample type: Serum,Tissue homogenate
        Reactivity: Mouse
      28. Interferon Regulatory Factor 1 Activates Autophagy to Aggravate Hepatic Ischemia-Reperfusion Injury by Increasing High Mobility Group Box 1 Release.
        IF: 5.5
        Journal: Cellular Physiology and Biochemistry(2018)
        DOI: 10.1159/000491732
        PMID: 30016764
        Products: E-EL-M0049 
        Sample type: Serum
        Reactivity: Mouse
      29. Gentiopicroside abrogates lipopolysaccharide-induced depressive-like behavior in mice through tryptophan-degrading pathway.
        IF: 2.441
        Journal: Metabolic Brain Disease
        DOI: 10.1007/s11011-018-0246-y
        PMID: 29948656
        Products: E-EL-M0037 E-EL-M0049 
        Sample type: Plasma
        Reactivity: Mouse
      30. Effects of Simulated Heat Wave and Ozone on High Fat Diet ApoE Deficient Mice.
        IF: 2.518
        Journal: Biomedical and Environmental Sciences(2018)
        DOI: 10.3967/bes2018.101
        PMID: 30423277
        Products: E-EL-M0049 E-EL-M0044 E-EL-M0077 E-EL-M0687 E-EL-M0048 E-EL-M2730 E-EL-M0400 E-EL-M0914 E-EL-0060 E-EL-M0419 E-EL-M0618 E-BC-K035-M E-BC-K020-M 
        Sample type: Plasma,Tissue homogenate
        Reactivity: Mouse
      31. Effect of Perillaldehyde on Prophylaxis and Treatment of Vaginal Candidiasis in a Murine Model
        IF: 4.259
        Journal: Frontiers in Microbiology(2019)
        DOI: 10.3389/fmicb.2019.01466
        PMID: 31333606
        Products: E-EL-M0047 E-EL-M2446 E-EL-M0049 
        Sample type: Tissue homogenate
        Reactivity: Mouse
      32. Effect of Perillaldehyde on Prophylaxis and Treatment of Vaginal Candidiasis in a Murine Model
        IF: 4.259
        Products: E-EL-M0047 E-EL-M2446 E-EL-M0049 
        Sample type: Tissue homogenates
        Reactivity: Mouse
      33. Galactan isolated from Cantharellus cibarius modulates antitumor immune response by converting tumor-associated macrophages toward M1-like phenotype
        IF: 6.044
        Journal: Carbohydrate Polymers(2019)
        DOI: 10.1016/j.carbpol.2019.115295
        PMID: 31582086
        Products: E-EL-M0049 
        Sample type: Cell culture supernatant
        Reactivity: Mouse
      34. NanoRNP Overcomes Tumor Heterogeneity in Cancer Treatment
        IF: 12.279
        Journal: NANO LETTERS(2019)
        DOI: 10.1021/acs.nanolett.9b02501
        PMID: 31593471
        Products: E-EL-M0692 E-EL-M3034 E-EL-M0695 E-EL-M0044 E-EL-M0048 E-EL-M0049 
        Sample type: Serum
        Reactivity: Mouse
      35. Design, Synthesis and Investigation of the Potential Anti-inflammatory Activity of 7-O-Amide Hesperetin Derivatives
        IF: 3.06
        Journal: Molecules(2019)
        DOI: 10.3390/molecules24203663
        PMID: 31614601
        Products: E-EL-M0037 E-EL-M0044 E-EL-M0049 
        Sample type: Cell culture supernatant
        Reactivity: Mouse
      36. NIR/ROS-Responsive Black Phosphorus QD Vesicles as Immunoadjuvant Carrier for Specific Cancer Photodynamic Immunotherapy
        IF: 15.6
        Journal: Advanced Functional Materials(2019)
        DOI: 10.1002/adfm.201905758
        Products: E-EL-M0044 E-EL-M0726 E-EL-M0049 
        Sample type: Serum
        Reactivity: Mouse
      37. Dual-Locking Nanoparticles Disrupt the PD-1/PD-L1 Pathway for Efficient Cancer Immunotherapy
        IF: 25.809
        Journal: Advanced materials(2019)
        DOI: 10.1002/adma.201905751
        PMID: 31709671
        Products: E-EL-0162 E-EL-M0048 E-EL-M0049 E-EL-M0042 E-EL-M0726 
        Sample type: Tissue homogenate
        Reactivity: Mouse
      38. Effect of Oral T2 Antigen on Chronic Prostatitis/ Chronic Pelvic Pain Syndrome in Mice Model
        IF: 2.939
        Journal: inflammation(2019)
        DOI: 10.1007/s10753-019-01072-4
        PMID: 31429013
        Products: E-EL-M0049 
        Sample type: Serum
        Reactivity: Mouse
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