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Mouse TNF-α(Tumor Necrosis Factor Alpha) ELISA Kit

  • Cat.No.:E-EL-M0049

  • Reactivity: Mouse

To Purchase E-EL-M0049

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 31.25-2000 pg/mL
Sensitivity 18.75 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Mouse TNF-α in samples. No significant cross-reactivity or interference between Mouse TNF-α and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Mouse TNF-α concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TNF-α. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TNF-α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TNF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse TNF-α. You can calculate the concentration of Mouse TNF-α in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse TNF-α were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse TNF-α were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 92.50 285.40 722.10 85.50 297.30 763.30
Standard deviation 6.00 16.60 33.90 4.50 11.90 41.20
CV (%) 6.49 5.82 4.69 5.26 4.00 5.40

Recovery

The recovery of Mouse TNF-α spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-111 101
EDTA plasma (n=8) 96-110 102
Cell culture media (n=8) 88-104 96

Linearity

Samples were spiked with high concentrations of Mouse TNF-α and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt: P06804
SynonymsDIF, TNF-alpha, TNFA, TNFSF2,TNF,TNFa
Research AreaCancer,Stem cells

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Related Products

Elabscience® provides ancillary reagents required for ELISA kit developments.11 reagents in total, including reagents for coating, blocking, washing, improving the stability and reducing matrix interference etc.
Cat.No. Product Name Specifications
E-ELIR-001 ELISA Plate Coating Buffer(1×) 100mL, 1000mL
E-ELIR-002 ELISA Plate Coating Buffer(5×) 100mL, 1000mL
E-ELIR-003 ELISA Plate Blocking Buffer 100mL, 1000mL
E-ELIR-004 Wash Buffer for Sandwich-ELISA(25×) 100mL, 1000mL
E-ELIR-005 Wash Buffer for Competitive-ELISA(25×) 100mL, 1000mL
E-ELIR-006 Stop Solution 100mL, 1000mL
E-ELIR-007 HRP-conjugate Stabilizer 10mL, 100mL, 500mL
E-ELIR-008 HRP-conjugate Diluent 100mL, 1000mL
E-ELIR-009 Biotinylated Antibody Stabilizer 10mL, 100mL, 500mL
E-ELIR-0010 Biotinylated Antibody Diluent 10mL, 100mL, 500mL
E-ELIR-0011 Sample Diluent 10mL, 100mL, 500mL

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

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    Sample: Tissue homogenate
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    Sample: Tissue homogenate
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    Sample: Tissue homogenate
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    Sample: Tissue homogenate
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    PMID: 31811349

    Sample: Serum
  78. Scientific reports (2020) IF: 3.998
    Latexin deficiency in mice up-regulates inflammation and aggravates colitis through HECTD1/Rps3/NF-κB pathway

    DOI: 10.1038/s41598-020-66789-x

    PMID: 32555320

    Sample: Serum
  79. Journal of Functional Foods (2020) IF: 3.701
    Anti-fatigue activity of purified anthocyanins prepared from purple passion fruit (P. edulis Sim) epicarp in mice

    DOI: 10.1016/j.jff.2019.103725

    PMID: 99999999

    Sample: Tissue homogenate
  80. Frontiers in Endocrinology (2020) IF: 3.644
    The Protective Effects of Melatonin Against LPS-Induced Septic Myocardial Injury: A Potential Role of AMPK-Mediated Autophagy

    DOI: 10.3389/fendo.2020.00162

    PMID: 32373063

    Sample: Serum
  81. European journal of pharmacology (2019) IF: 3.263
    Circular RNA expression profile of liver tissues in an EtOH-induced mouse model of alcoholic hepatitis

    DOI: 10.1016/j.ejphar.2019.172642

    PMID: 31493407

    Sample: Serum,Tissue homogenate
  82. Journal of biochemical and molecular toxicology (2019) IF: 3.606
    Gastrodin alleviates Tourette syndrome via Nrf-2/HO-1/HMGB1/NF-кB pathway

    DOI: 10.1002/jbt.22389

    PMID: 31468582

    Sample: Tissue homogenate
  83. International immunopharmacology (2019) IF: 3.943
    Regulation of CD39 expression in ATP-P2Y2R-mediated alcoholic liver steatosis and inflammation

    DOI: 10.1016/j.intimp.2019.105915

    PMID: 31639617

    Sample: Serum,Cell culture supernatant
  84. European journal of pharmacology (2019) IF: 3.263
    Pancreastatin inhibitor PSTi8 attenuates hyperinsulinemia induced obesity and inflammation mediated insulin resistance via MAPK/NOX3-JNK pathway

    DOI: 10.1016/j.ejphar.2019.172723

    PMID: 31586632

    Sample: Plasma,Cell culture supernatant,Tissue homogenate
  85. American Journal of Translational Research (2019) IF: 3.375
    Xiaoyaosan prevents atherosclerotic vulnerable plaque formation through heat shock protein/glucocorticoid receptor axis-mediated mechanism

    DOI: 99999999

    PMID: 31632527

    Sample: Serum
  86. Investigative ophthalmology & visual science (2018) IF: 3.27
    Aspergillus fumigatus Increased PAR-2 Expression and Elevated Proinflammatory Cytokines Expression Through the Pathway of PAR-2/ERK1/2 in Cornea

    DOI: 10.1167/iovs.17-21887

    PMID: 29332129

    Sample: Tissue homogenate
  87. International Immunopharmacology (2020) IF: 3.361
    10-Hydroxydecanoic acid inhibits LPS-induced inflammation by targeting p53 in microglial cells

    DOI: 10.1016/j.intimp.2020.106501

    PMID: 99999999

    Sample: Cell culture supernatant
  88. International immunopharmacology (2018) IF: 3.118
    Interaction of mannose binding lectin and other pattern recognition receptors in human corneal epithelial cells during Aspergillus fumigatus infection

    DOI: 10.1016/j.intimp.2018.08.003

    PMID: 30096599

    Sample: Tissue homogenate
  89. Frontiers in pharmacology (2018) IF: 3.831
    Emodin attenuates lipopolysaccharide-induced acute liver injury through inhibiting the TLR4 signaling pathway in vitro and in vivo

    DOI: 10.3389/fphar.2018.00962

    PMID: 30186181

    Sample: Cell culture supernatant
  90. Bioorganic chemistry (2019) IF: 3.929
    Assessment of novel azaanthraquinone derivatives as potent multi-target inhibitors of inflammation and amyloid-β aggregation in Alzheimer's disease

    DOI: 10.1016/j.bioorg.2018.10.073

    PMID: 30448726

    Sample: Cell culture medium
  91. Life sciences (2019) IF: 3.234
    GPR 30 reduces myocardial infarct area and fibrosis in female ovariectomized mice by activating the PI3K/AKT pathway

    DOI: 10.1016/j.lfs.2019.03.049

    PMID: 30905784

    Sample: Serum
  92. American journal of translational research (2019) IF: 3.061
    Vascular endothelial Cdc42 deficiency delays skin wound-healing processes by increasing IL-1β and TNF-α expression

    DOI: 99999999

    PMID: 30787984

    Sample: Tissue homogenate
  93. Toxicology and applied pharmacology (2019) IF: 3.616
    SIRT1 activation by butein attenuates sepsis-induced brain injury in mice subjected to cecal ligation and puncture via alleviating inflammatory and oxidative stress

    DOI: 10.1016/j.taap.2018.10.013

    PMID: 30336174

    Sample: Serum,Tissue homogenate
  94. Molecules (2018) IF: 3.098
    Nuciferine Inhibits Proinflammatory Cytokines via the PPARs in LPS-Induced RAW264. 7 Cells

    DOI: 10.3390/molecules23102723

    PMID: 30360404

    Sample: Cell culture supernatant
  95. International journal of molecular sciences (2019) IF: 3.687
    Salidroside Ameliorates Renal Interstitial Fibrosis by Inhibiting the TLR4/NF-κB and MAPK Signaling Pathways

    DOI: 10.3390/ijms20051103

    PMID: 30836660

    Sample: Serum
  96. Toxicology (2019) IF: 3.265
    4-Methylcoumarin-[5,6–g]-hesperetin attenuates inflammatory responses in alcoholic hepatitis through PPAR-γ activation

    DOI: 10.1016/j.tox.2019.04.004

    PMID: 30951781

    Sample: Serum,Cell culture supernatant
  97. Molecules (2019) IF: 3.06
    Design, Synthesis and Investigation of the Potential Anti-inflammatory Activity of 7-O-Amide Hesperetin Derivatives

    DOI: 10.3390/molecules24203663

    PMID: 31614601

    Sample: Cell culture supernatant
  98. Biomedicine & Pharmacotherapy (2018) IF: 3.457
    The protective effect of formononetin on cognitive impairment in streptozotocin (STZ)-induced diabetic mice.

    DOI: 10.1016/j.biopha.2018.07.063

    PMID: 30119194

    Sample: Serum,Tissue homogenate
  99. Journal of Biomedical Materials Research Part A (2015) IF: 3.076
    Dendritic Cells Enhance UHMWPE Wear Particle-Induced Osteoclast Differentiation Of Macrophages.

    DOI: 10.1002/jbm.a.35459

    PMID: 25808788

    Sample: Cell culture supernatant
  100. Neuroscience (2017) IF: 3.277
    Blocking The CD38/cADPR Pathway Plays A Double-edged Role In LPS Stimulated Microglia.

    DOI: 10.1016/j.neuroscience.2017.08.010

    PMID: 28807785

    Sample: Cell culture supernatant
  101. Acta Pharmacologica Sinica (2017) IF: 3.223
    Molecular Mechanisms Of the Analgesic Action Of Wu-tou Decoction On Neuropathic Pain In Mice Revealed Using Microarray And Network Analysis.

    DOI: 10.1038/aps.2017.110

    PMID: 28816231

    Sample: Tissue homogenate
  102. Journal of Functional Foods (2017) IF: 3.144
    Antitumor And Immunomodulatory Effects Of Ginsenoside Rh2 And Its Octyl Ester Derivative In H22 Tumor-Bearing Mice.

    DOI: 10.1016/j.jff.2017.03.013

    Sample: Serum
  103. Food & Function (2017) IF: 3.247
    Purification, Characterization And Bioactivities Of Polysaccharides From Pleurotus Ferulae.

    DOI: 10.1039/c7fo00227k

    PMID: 28436508

    Sample: Cell culture supernatant
  104. International Journal of Chronic Obstructive Pulmonary Disease (2017) IF: 3.157
    Repression Of Toll-Like Receptor-4 By microRNA-149-3p Is Associated With Smoking-Related COPD.

    DOI: 10.2147/COPD.S128031

    PMID: 28260877

    Sample: Cell culture supernatant
  105. International Journal of Molecular Sciences (2015) IF: 3.226
    Inhibition Of Hepatocyte Apoptosis: An Important Mechanism Of Corn Peptides Attenuating Liver Injury Induced By Ethanol.

    DOI: 10.3390/ijms160922062

    PMID: 26378531

    Sample: Serum,Tissue homogenate
  106. Plants (2020) IF: 2.762
    Phytochemical, Cytotoxicity, Antioxidant and Anti-Inflammatory Effects of Psilocybe Natalensis Magic Mushroom

    DOI: 10.3390/plants9091127

    PMID: 32878164

    Sample: Cell culture supernatant
  107. Journal of Neuroscience Methods (2020) IF: 2.214
    Frequent exposure to varied home cage sizes alters pain sensitivity and some key inflammation-related biomarkers

    DOI: 10.1016/j.jneumeth.2020.108890

    PMID: 32768413

    Sample: Tissue homogenate
  108. Bioscience Reports (2020) IF: 2.942
    Atractylon treatment prevents sleep-disordered breathing-induced cognitive dysfunction by suppression of chronic intermittent hypoxia-induced M1 microglial activation

    DOI: 10.1042/BSR20192800

    PMID: 32490526

    Sample: Cell culture supernatant
  109. BioMed Research International (2020) IF: 2.276
    Irisin Pretreatment Protects Kidneys against Acute Kidney Injury Induced by Ischemia/Reperfusion via Upregulating the Expression of Uncoupling Protein 2

    DOI: 10.1155/2020/6537371

    PMID: 32934963

    Sample: Tissue homogenate
  110. Molecular Medicine Reports (2020) IF: 2.1
    Osthole inhibits proliferation of kainic acid-activated BV-2 cells by modulating the Notch signaling pathway

    DOI: 10.3892/mmr.2020.11455

    PMID: 33000274

    Sample: Cell culture supernatant
  111. Journal of Pharmacy and Pharmacology (2020) IF: 2.571
    Therapeutic effects of Qianlie Tongli decoction on chronic prostatitis/chronic pelvic pain syndrome induced by peptide T2 in mice

    DOI: 10.1111/jphp.13325

    PMID: 32648958

    Sample: Plasma
  112. Immunopharmacology and Immunotoxicology (2020) IF: 2.352
    Kupffer cell inactivation ameliorates immune liver injury via TNF-α/TNFR1 signal pathway in trichloroethylene sensitized mice

    DOI: 10.1080/08923973.2020.1811306

    PMID: 32811237

    Sample: Serum
  113. Brain and behavior (2020) IF: 2.091
    MicroRNA-345-5p regulates depression by targeting suppressor of cytokine signaling 1

    DOI: 10.1002/brb3.1653

    PMID: 32730696

    Sample: Cell culture supernatant
  114. Molecular Medicine Reports (2020) IF: 2.1
    Placenta-derived mesenchymal stem cells ameliorate lipopolysaccharide-induced inflammation in RAW264. 7 cells and acute lung injury in rats

    DOI: 10.3892/mmr.2020.11231

    PMID: 32626979

    Sample: Cell culture supernatant
  115. Microbial Pathogenesis (2020) IF: 2.914
    Exposure time determines the protective effect of Trichinella spiralis on experimental colitis

    DOI: 10.1016/j.micpath.2020.104263

    PMID: 32442663

    Sample: Serum
  116. Microbial Pathogenesis (2020) IF: 2.914
    Curcumin ameliorates Staphylococcus aureus-induced mastitis injury through attenuating TLR2-mediated NF-κB activation

    DOI: 10.1016/j.micpath.2020.104054

    PMID: 32061917

    Sample: Serum
  117. Molecular Medicine Reports (2020) IF: 2.1
    Coenzyme Q10 alleviates sevoflurane-induced neuroinflammation by regulating the levels of apolipoprotein E and phosphorylated tau protein in mouse hippocampal neurons

    DOI: 10.3892/mmr.2020.11131

    PMID: 32377738

    Sample: Tissue homogenate
  118. PeerJ (2020) IF: 2.379
    The immunostimulatory activity of polysaccharides from Glycyrrhiza uralensis

    DOI: 10.7717/peerj.8294

    PMID: 32030319

    Sample: Tissue homogenate
  119. Poultry science (2019) IF: 2.659
    Anti-inflammatory effect of preserved egg with simulated gastrointestinal digestion on LPS-stimulated RAW264.7 cells

    DOI: 10.3382/ps/pez243

    PMID: 31041437

    Sample: Cell culture supernatant
  120. Molecular medicine reports (2018) IF: 2.1
    Dioscin prevents LPS-induced acute lung injury through inhibiting the TLR4/MyD88 signaling pathway via upregulation of HSP70

    DOI: 10.3892/mmr.2018.8667

    PMID: 29512786

    Sample: Serum,Tissue homogenate
  121. Medicine (2018) IF: 2.028
    Effect of gap junctions on RAW264. 7 macrophages infected with H37Rv

    DOI: 10.1097/MD.0000000000012125

    PMID: 30170447

    Sample: Cell culture supernatant
  122. Inflammation (2018) IF: 2.884
    miR-590-3p Attenuates Acute Kidney Injury by Inhibiting Tumor Necrosis Factor Receptor-Associated Factor 6 in Septic Mice

    DOI: 10.1007/s10753-018-0921-5

    PMID: 30392071

    Sample: Serum
  123. Biochemical and biophysical research communications (2019) IF: 2.559
    Eosinophils attenuate arthritis by inducing M2 macrophage polarization via inhibiting the IkB/P38 MAPK signaling pathway

    DOI: 10.1016/j.bbrc.2018.12.010

    PMID: 30528734

    Sample: Tissue homogenate,Cell culture supernatant
  124. Inflammation (2018) IF: 2.884
    Resveratrol Protects Against Renal Damage via Attenuation of Inflammation and Oxidative Stress in High-Fat-Diet-Induced Obese Mice

    DOI: 10.1007/s10753-018-0948-7

    PMID: 30554371

    Sample: Tissue homogenate
  125. Molecular diversity (2019) IF: 2.229
    Hybrids of oxoisoaporphine-tetrahydroisoquinoline: novel multi target inhibitors of infammation and amyloid-β aggregation in Alzheimer's disease

    DOI: 10.1007/s11030-018-9905-5

    PMID: 30603938

    Sample: Cell culture medium
  126. Steroids (2019) IF: 2.523
    Synthesis and Evaluation of Novel D-ring Substituted Steroidal Pyrazolines as Potential Anti-inflammatory Agents

    DOI: 10.1016/j.steroids.2019.03.012

    PMID: 30951758

    Sample: Cell culture supernatant
  127. Journal of the Science of Food and Agriculture (2019) IF: 2.379
    Effect and mechanism of citral against methicillin-resistant Staphylococcus aureus in vivo

    DOI: 10.1002/jsfa.9677

    PMID: 30891759

    Sample: Serum
  128. Journal of cellular biochemistry (2019) IF: 2.959
    Attractylone Attenuates Sepsis-Associated Encephalopathy and Cognitive Dysfunction by Inhibiting Microglial Activation and Neuroinflammation

    DOI: 10.1002/jcb.27983

    PMID: 30672013

    Sample: Cell culture supernatant,Tissue homogenate
  129. PloS one (2018) IF: 2.766
    AT1-receptor blockade: Protective effects of irbesartan in cardiomyocytes under hypoxic stress

    DOI: 10.1371/journal.pone.0202297

    PMID: 30356256

    Sample: Cell culture supernatant
  130. Gene (2019) IF: 2.498
    The inhibition of microRNA-203 on ischemic reperfusion injury after total knee arthroplasty via suppressing MYD88-mdiated toll-like receptor signaling pathway

    DOI: 10.1016/j.gene.2019.02.030

    PMID: 30772517

    Sample: Tissue homogenate
  131. Inflammation (2019) IF: 2.884
    Sinomenine Attenuates Cartilage Degeneration by Regulating miR-223-3p/NLRP3 Inflammasome Signaling

    DOI: 10.1007/s10753-019-00986-3

    PMID: 30847744

    Sample: Serum,Cell culture supernatant
  132. inflammation (2019) IF: 2.939
    Effect of Oral T2 Antigen on Chronic Prostatitis/ Chronic Pelvic Pain Syndrome in Mice Model

    DOI: 10.1007/s10753-019-01072-4

    PMID: 31429013

    Sample: Serum
  133. Biomedical and Environmental Sciences (2018) IF: 2.518
    Effects of Simulated Heat Wave and Ozone on High Fat Diet ApoE Deficient Mice.

    DOI: 10.3967/bes2018.101

    PMID: 30423277

    Sample: Plasma,Tissue homogenate
  134. Metabolic Brain Disease IF: 2.441
    Gentiopicroside abrogates lipopolysaccharide-induced depressive-like behavior in mice through tryptophan-degrading pathway.

    DOI: 10.1007/s11011-018-0246-y

    PMID: 29948656

    Sample: Plasma
  135. International Immunopharmacology (2017) IF: 2.956
    Wogonin Attenuates Inflammation By Activating PPAR-γ In Alcoholic Liver Disease.

    DOI: 10.1016/j.intimp.2017.06.013

    PMID: 28646664

    Sample: Serum,Cell culture supernatant
  136. International Immunopharmacology (2016) IF: 2.956
    MiR-146a Modulates Macrophage Polarization By Inhibiting Notch1 Pathway In RAW264. 7 Macrophages.

    DOI: 10.1016/j.intimp.2016.01.009

    PMID: 26800097

    Sample: Cell culture supernatant
  137. Molecules (2017) IF: 2.861
    Capparis Spinosa Fruit Ethanol Extracts Exert Different Effects On The Maturation Of Dendritic Cells.

    DOI: 10.3390/molecules22010097

    PMID: 28067853

    Sample: Cell culture supernatant
  138. Placenta (2015) IF: 2.759
    The Effect Of Maternal Obesity On The Expression And Functionality Of Placental P-Glycoprotein: Implications In The Individualized Transplacental Digoxin Treatment For Fetal Heart Failure.

    DOI: 10.1016/j.placenta.2015.08.007

    PMID: 26311557

    Sample: Plasma
  139. Parasitology Research (2016) IF: 2.329
    Immunopathological Assessments Of Human Blastocystis Spp. In Experimentally Infected Immunocompetent And Immunosuppresed Mice.

    DOI: 10.1007/s00436-016-4951-3

    PMID: 26860840

    Sample: Serum,Tissue homogenate
  140. Biochemical & Biophysical Research Communications (2015) IF: 2.466
    Ghrelin Ameliorates Intestinal Barrier Dysfunction In Experimental Colitis By Inhibiting The Activation Of Nuclear Factor-Kappa B.

    DOI: 10.1016/j.bbrc.2015.01.083

    PMID: 25634696

    Sample: Tissue homogenate
  141. Experimental and Therapeutic Medicine (2020) IF: 1.785
    Clostridium butyricum protects intestinal barrier function via upregulation of tight junction proteins and activation of the Akt/mTOR signaling pathway in a mouse model of dextran sodium sulfate-induced colitis

    DOI: 10.3892/etm.2020.9138

    PMID: 32934675

    Sample: Serum
  142. Current Medical Science (2020) IF: 1.273
    Role of Interleukin 17A in Aortic Valve Inflammation in Apolipoprotein E-deficient Mice

    DOI: 10.1007/s11596-020-2230-0

    PMID: 32862384

    Sample: Plasma
  143. Asian Pacific Journal of Tropical Medicine (2019) IF: 1.94
    Alpinia officinarum Hance extract alleviates particulate matter-induced lung injury in mice

    DOI: 10.4103/1995-7645.272487

    PMID: 99999999

    Sample: Serum,Bronchoalveolar Lavage Fluid,Tissue homogena
  144. Experimental and Therapeutic Medicine (2020) IF: 1.785
    Curcumin affects ox-LDL-induced IL-6, TNF-α, MCP-1 secretion and cholesterol efflux in THP-1 cells by suppressing the TLR4/NF-κB/miR-3a signaling pathway

    DOI: 10.3892/etm.2020.8915

    PMID: 99999999

    Sample: Cell culture supernatant
  145. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research (2020) IF: 1.918
    The Effects of RKI-1447 in a Mouse Model of Nonalcoholic Fatty Liver Disease Induced by a High-Fat Diet and in HepG2 Human Hepatocellular Carcinoma Cells Treated with Oleic Acid

    DOI: 10.12659/MSM.919220

    PMID: 32026851

    Sample: Serum,Cell culture supernatant,Tissue homogenate
  146. International journal of ophthalmology (2019) IF: 1.33
    Expression and role of calcitonin gene-related peptide in mouse Aspergillus fumigatus keratitis

    DOI: 10.18240/ijo.2019.05.01

    PMID: 31131225

    Sample: Cell culture supernatant
  147. Current medical science (2019) IF: 1.273
    Berberine Attenuates Cigarette Smoke Extract-induced Airway Inflammation in Mice: Involvement of TGF-β1/Smads Signaling Pathway

    DOI: 10.1007/s11596-019-2101-8

    PMID: 31612392

    Sample: Bronchoalveolar Lavage Fluid
  148. Canadian journal of physiology and pharmacology (2019) IF: 1.946
    Role of Brd4 in the production of inflammatory cytokines in mouse macrophages treated with titanium particles

    DOI: 10.1139/cjpp-2019-0142

    PMID: 31330113

    Sample: Cell culture supernatant
  149. Journal of Food Biochemistry (2019) IF: 1.662
    Immunomodulatory effect of papaya (Carica papaya) pulp and seed extracts as a potential natural treatment for bacterial stress

    DOI: 10.1111/jfbc.13050

    PMID: 31571245

    Sample: Serum
  150. Neuroreport (2018) IF: 1.394
    Gentiopicroside protects neurons from astrocyte-mediated inflammatory injuries by inhibition of nuclear factor-κB and mitogen-activated protein kinase signaling pathways

    DOI: 10.1097/WNR.0000000000001082

    PMID: 29958245

    Sample: Cell culture supernatant
  151. NeuroReport (2018) IF: 1.266
    Role of receptor-interacting protein 1/receptor-interacting protein 3 in inflammation and necrosis following chronic constriction injury of the sciatic nerve

    DOI: 10.1097/WNR.0000000000001120

    PMID: 30192300

    Sample: Tissue homogenate
  152. Aesthetic plastic surgery (2019) IF: 1.484
    M1/M2 Macrophages Play Different Roles in Adipogenic Differentiation of PDGFRα+ Preadipocytes In Vitro

    DOI: 10.1007/s00266-018-1294-8

    PMID: 30552469

    Sample: Cell culture supernatant
  153. Medical molecular morphology (2019) IF: 1.217
    The therapeutic effects of resveratrol on hepatic steatosis in high-fat diet-induced obese mice by improving oxidative stress, inflammation and lipid-related gene transcriptional expression

    DOI: 10.1007/s00795-019-00216-7

    PMID: 30673851

    Sample: Serum,Tissue homogenate
  154. Aerobiologia (2019) IF: 1.515
    Aerofloral investigation and allergenic potentials of two dominant airborne pollen types at selected sites in South-western Nigeria

    DOI: 10.1007/s10453-018-9533-7

    PMID: 99999999

    Sample: Plasma
  155. Cellular & molecular biology letters (2019) IF: 1.291
    Tetrahydroxy stilbene glucoside alleviates palmitic acid-induced inflammation and apoptosis in cardiomyocytes by regulating miR-129-3p/Smad3 signaling

    DOI: 10.1186/s11658-018-0125-x

    PMID: 30820195

    Sample: Cell culture supernatant
  156. Journal of biochemical and molecular toxicology (2019) IF: 1.837
    Gastrodin attenuates neuroinflammation in DOI-induce Tourette syndrome in rats

    DOI: 10.1002/jbt.22302

    PMID: 30790395

    Sample: Serum,Tissue homogenate
  157. Cellular & molecular biology letters (2019) IF: 1.291
    Honokiol alleviates sepsis-induced acute kidney injury in mice by targeting the miR-218-5p/heme oxygenase-1 signaling pathway

    DOI: 10.1186/s11658-019-0142-4

    PMID: 30833971

    Sample: Serum
  158. BMC Pharmacology and Toxicology (2020) IF: 1.771
    Impact of Lesinurad and allopurinol on experimental Hyperuricemia in mice: biochemical, molecular and Immunohistochemical study

    DOI: 10.1186/s40360-020-0386-7

    PMID: 32041665

    Sample: Serum
  159. Oncology Letters (2018) IF: 1.39
    The Association Between Inflammation, The Microbiome and Urethane-induced Pulmonary Adenocarcinoma.

    DOI: 10.3892/ol.2018.8167

    PMID: 29725395

  160. Experimental and Therapeutic Medicine (2017) IF: 1.261
    Effects Of Sepsis On The Metabolism Of Sphingomyelin And Cholesterol In Mice With Liver Dysfunction.

    DOI: 10.3892/etm.2017.5226

    PMID: 29285103

    Sample: Plasma
  161. International Journal of Clinical and Experimental Medicine (2015) IF: 1.069
    Sulfated Modification Promotes The Immunomodulatory Bioactivities Of Lyciumbarbarum Polysaccharides In Vitro.

    PMID: 26884954

    Sample: Cell culture supernatant
  162. Journal of Oleo Science (2015) IF: 1.076
    A Comparative Study Of The Effects Upon LPS Induced Macrophage RAW264.7 Inflammation In Vitro Of The Lipids Of Hippocampus Trimaculatus Leach.

    DOI: 10.5650/jos.ess15190

    PMID: 26582156

    Sample: Cell culture supernatant
  163. Porto Biomedical Journal (2020)
    An albino mouse model of nonalcoholic fatty liver disease induced using high-fat liquid "Lieber-DeCarli" diet: a preliminary investigation

    DOI: 10.1097/j.pbj.0000000000000071

    PMID: 32734012

    Sample: Plasma,Tissue homogenate
  164. Medical Science Monitor: International Medical Journal of Experimental and Clinical Research (2020)
    Methamphetamine induced neuroinflammation in mouse brain and microglial cell line BV2: Roles of the TLR4/TRIF/Peli1 signaling axis

    DOI: 10.1016/j.toxlet.2020.07.028

    PMID: 32768653

    Sample: Cell culture supernatant,Tissue homogenate
  165. Advances in Pharmacological and Pharmaceutical Sciences (2020)
    Antihypernociceptive, Anxiolytic, and Antidepressant Properties of Aqueous and Ethanol Extracts of Dissotis thollonii Cogn. (Melastomataceae) in Mice

    DOI: 10.1155/2020/8886894

    PMID: 33015629

    Sample: Serum,Tissue homogenate
  166. bioRxiv (2020)
    Fecal microbiota transplantation reconstructs the gut microbiota of septic mice and protects the intestinal mucosal barrier

    DOI: 10.1101/2020.06.22.164541

    PMID: 99999999

    Sample: Serum
  167. Annals of Medical Research (2020)
    Effects of exercise on netrin-1 and TNF-α levels in non-inflammatory conditions

    DOI: 10.5455/annalsmedres.2019.12.798

    PMID: 99999999

    Sample: Tissue homogenate
  168. Indian Journal of Public Health Research & Development (2019)
    Efficacy of Topical Cream of Garlic Extract (Allium Sativum) on Wound Healing in Experimental Mice using Aa Acute> Wound Modeling: Determination of Expresión of Tumor Necrotic Factor (TNF-α)

    DOI: 10.5958/0976-5506.2019.03028.6

    PMID: 99999999

    Sample: Tissue homogenate
  169. SSRN (2020)
    Salidroside Attenuates Liver Injury in Diabetes Mice

    DOI: 10.2139/ssrn.3546025

    PMID: 99999999

    Sample: Serum,Cell culture supernatant,Tissue homogenate
  170. Journal of Cellular and Molecular Medicine (2020)
    Polysaccharides from Dicliptera chinensis ameliorate liver disturbance by regulating TLR-4/NF-κB and AMPK/Nrf2 signalling pathways

    DOI: 10.1111/jcmm.15286

    PMID: 32337831

    Sample: Serum,Tissue homogenate
  171. Open access Macedonian journal of medical sciences (2019)
    Neuroprotective Activity of Evolvulus alsinoides & Centella asiatica Ethanolic Extracts in Scopolamine-Induced Amnesia in Swiss Albino Mice

    DOI: 10.3889/oamjms.2019.247

    PMID: 31049081

    Sample: Tissue homogenate
  172. Iraqi Journal of Pharmaceutical Sciences (2018)
    Ameliorating Effect of Oral Guggulsterone Administration in Imiquimod-Induced Psoriasis in Mice

    DOI: 99999999

    PMID: 99999999

    Sample: Serum,Tissue homogenate
  173. Naunyn-Schmiedeberg's archives of pharmacology (2019)
    Antihypernociceptive and neuroprotective effects of Combretin A and Combretin B on streptozotocin-induced diabetic neuropathy in mice.

    DOI: 10.1007/s00210-019-01626-1

    PMID: 30759263

    Sample: Serum,Spinal cord
  174. Preprints (2019)
    Ameliorative Effects of Lesinurad (Zurampic) on Experimental Hyperuricemia, Biochemical, Molecular and Immunohistochemical Study

    DOI: 10.21203/rs.2.17096/v1

    PMID: 99999999

    Sample: Serum
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