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Myeloperoxidase (MPO) Activity Assay Kit

Name: Myeloperoxidase (MPO) Activity Assay Kit
Brand: Elabscience
SKU: E-BC-K074-S
Price: 180 USD
Availability: InStock
Rating: 5 (1 reviews)

Citations(21)

Cat.No.:E-BC-K074-S
Detection instrument: Spectrophotometer(460 nm)

Detection method: Colorimetric method

Manual MSDS

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100Assays

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Detection principle

Myeloperoxidase reduces hydrogen peroxide to a complex. The complex react with o-dianisidine (as hydrogen donor) to produce a yellow product which has a maximum absorption peak at 460 nm. The activity of MPO can be calculated indirectly by measuring the OD value at 460nm.

Performance characteristics

Synonyms	MPO
Sample type	Serum,plasma,milk,animal tissue,cells
Sensitivity	16.95 U/L
Detection range	16.95-3349 U/L
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	90 min
Precision	Average inter-assay CV: 9.8%Average intra-assay CV: 4.5%
Other instruments required	Vortex mixer, Micropipettor, Water bath, Incubator, Centrifuge
Storage	2-8℃
Valid period	12 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (4.9-196.7 U/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Human plasma	1
Rat serum	1
5% Mouse brain tissue homogenization	1
5% Mouse heart tissue homogenization	1
Human milk	1

Note: The diluent is reagent 2 application solution.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Frontiers in Immunology (2021) IF: 7.561
Transcriptional Profiling and Functional Analysis of N1/N2 Neutrophils Reveal an Immunomodulatory Effect of S100A9-Blockade on the Pro-Inflammatory N1 Subpopulation

DOI: 10.3389/fimmu.2021.708770

Sample: neutrophils
Journal of Inflammation Research (2022) IF: 6.922
Nociceptor Neurons are Involved in the Host Response to Escherichia coli Urinary Tract Infections

DOI: 10.2147/JIR.S356960

Sample: Neutrophil
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES (2022) IF: 5.112
Thermosensitive smart hydrogel of PEITC ameliorates the therapeutic efficacy in rheumatoid arthritis

DOI: 10.1016/j.ejps.2022.106367

Sample: serum
CHEMICO-BIOLOGICAL INTERACTIONS (2022) IF: 5.194
PAR1, a therapeutic target for remote lung injury associated with hind limb ischemia/reperfusion: ERK5/KLF2-dependent lung capillary barrier preservation

DOI: 10.1016/j.cbi.2022.109809

PMID: 35031271
Sample: lung
SAUDI JOURNAL OF BIOLOGICAL SCIENCES (2022) IF: 4.052
Evaluation of testicular torsion management in Ogbomoso, South-Western Nigeria and surgical detorsion-augmented treatment with phytochemical fractions of Corchorus olitorius leaf in experimental rats

DOI: 10.1016/j.sjbs.2022.103495

Sample: testicular tissue
Foods (2022) IF: 4.35
Protective Effects of a Novel Probiotic Bifidobacterium pseudolongum on the Intestinal Barrier of Colitis Mice via Modulating the Pparγ/STAT3 Pathway and Intestinal Microbiota

DOI: 10.3390/foods11111551

Sample: colon tissue
JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36
Revealing the mechanism of “Huai Hua San” in the treatment of ulcerative colitis based on network pharmacology and experimental study

DOI: 10.1016/j.jep.2021.114321

Sample: colon
Scientific Reports (2022) IF: 4.38
Saffron extract and crocin exert anti-inflammatory and anti-oxidative effects in a repetitive mild traumatic brain injury mouse model

DOI: 10.1038/s41598-022-09109-9

PMID: 35322143
Sample: cortex
Scientific Reports (2022) IF: 4.38
Promotion of the inflammatory response in mid colon of complement component 3 knockout mice

DOI: 10.1038/s41598-022-05708-8

PMID: 35105928
Sample: colon
FASEB JOURNAL (2020) IF: 4.966
CaMK4‐dependent phosphorylation of Akt/mTOR underlies Th17 excessive activation in experimental autoimmune prostatitis

DOI: 10.1096/fj.201902910RRR

Sample: Cell culture supernatant,Tissue homogenate
PHYTOTHERAPY RESEARCH (2017) IF: 3.092
Protective effect of gentiopicroside from Gentiana macrophylla Pall. in ethanol‐induced gastric mucosal injury in mice

DOI: 10.1002/ptr.5965

Sample: Tissue homogenate
DISEASE MARKERS (2022) IF: 3.434
Knockdown of ILK Alleviates High Glucose-Induced Damage of H9C2 Cells through TLR4/MyD88/NF-κB Pathway

DOI: 10.1155/2022/6205190

Sample: H9C2 cell
LIFE SCIENCES (2020) IF: 3.647
Attenuation of chronic arsenic neurotoxicity via melatonin in male offspring of maternal rats exposed to arsenic during conception: Involvement of oxidative DNA damage and inflammatory signaling cascades

DOI: 10.1016/j.lfs.2020.118876

PMID: 33310035
Sample: tissue
BIOGERONTOLOGY (2020) IF: 3.667
d -galactose: a model of accelerated ageing sufficiently sensitive to reflect preventative efficacy of an antioxidant treatment

DOI: 10.1007/s10522-020-09891-x

Sample: Tissue homogenate
Immunity Inflammation and Disease (2021) IF: 2.239
Neutrophil extracellular traps induce thrombogenicity in severe carotid stenosis

DOI: 10.1002/iid3.466

PMID: 34102007
Sample: plasma
BMC Complementary and Alternative Medicine (2017) IF: 2.288
Extract from mango mistletoes Dendrophthoe pentandra ameliorates TNBS-induced colitis by regulating CD4+ T cells in mesenteric lymph nodes

DOI: 10.1186/s12906-017-1973-z

Sample: neutrophils
Journal of Experimental Zoology Part A-Ecological and Integrative Physiology (2022) IF: 2.693
Molecular mechanisms underlying ameliorative impact of melatonin against age-dependent chronic arsenic toxicity in rats' brains

DOI: 10.1002/jez.2603

Sample: brain
ANDROLOGIA (2022) IF: 2.775
IL-17 exacerbates experimental autoimmune prostatitis via CXCL1/CXCL2-mediated neutrophil infiltration

DOI: 10.1111/and.14455

PMID: 35560069
Sample: prostate tissue
BMC Complementary and Alternative Medicine (2020) IF: 2.833
Shen-ling-bai-zhu-san ameliorates inflammation and lung injury by increasing the gut microbiota in the murine model of Streptococcus pneumonia-induced pneumonia.

DOI: 10.1186/s12906-020-02958-9

Sample: Tissue homogenate
LIFE SCIENCES (2017) IF: 2.936
Gastroprotective effect of aucubin against ethanol-induced gastric mucosal injury in mice

DOI: 10.1016/j.lfs.2017.09.016

Sample: Tissue homogenate
Molecular Medicine Reports (2021) IF: 2.952
Protective effect of parecoxib sodium against ischemia reperfusion‑induced intestinal injury

DOI: 10.3892/mmr.2021.12416

PMID: 34498709
Sample: serum,small intestinal

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Verified Customer

V****lSubmitted [ Feb 05 2020 ]

Application:Biochemical
Description:The procedure of the kit very quick, hence assay was completed within an hour, but we have sample ready. The assay can be performed with half the size of the plasma.

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M*****aSubmitted [ Oct 20 2022 ]

Asked: Is there a way to prolong the shelf life to 12 months?

adminSubmitted [ Oct 20 2022 ]

Answered: Sorry, There is no way to extend the shelf life, which depends on the properties of the reagent.

M*****aSubmitted [ Sep 16 2022 ]

Asked: How many reactions can be done with this kit as you have mentioned 100 Assays (Can detect 50 samples without duplication) Can you please clarify.

adminSubmitted [ Sep 16 2022 ]

Answered: Because each sample is required for a control, it only detect 50 samples. 100Assays=50 sample tubes +50 control tubes （each sample need a control）

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