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Myeloperoxidase (MPO) Activity Assay Kit

Cat.No.:E-BC-K074-M
Detection instrument: Microplate reader(450 nm-470 nm,optimum wavelength: 460 nm)

Detection method: Colorimetric method

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Detection principle

Myeloperoxidase reduces hydrogen peroxide to a complex. The complex react with o-dianisidine (as hydrogen donor) to produce a yellow product which has a maximum absorption peak at 460 nm. The activity of MPO can be calculated indirectly by measuring the OD value at 460nm.

Performance characteristics

Synonyms	MPO
Sample type	Serum, plasma, animal tissue
Sensitivity	19.42 U/L
Detection range	19.42-893.31 U/L
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	65 min
Precision	Average inter-assay CV: 7.300%Average intra-assay CV: 5.400%
Other instruments required	Tubes, Micropipette, Vortex mixer, 37℃/60℃ Water bath
Storage	2-8℃
Valid period	12 months

Images

The activity of MPO indicating the neutrophil level was slightly higher in the KO (C3 knockout) than WT (wild type) mice.

Y J Choi investigate the effective of complement component 3 (C3) deficiency promoting its receptor downstream-mediated inflammatory response. Myeloperoxidase (MPO) activity of mouse colon was determined using MPO activity assay kit (E-BC-K074-M).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (19.42-893.31 U/L).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
Human serum	1
Human plasma	1
Human milk	1
Cell culture supernatant	1
Rat serum	1
Rat plasma	1
5% Rat kidney tissue homogenate	1
5% Rat spleen tissue homogenate	1

Note: The diluent is reagent 2 application solution.

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Small (2023) IF: 15.153
Oral Metal-Free Melanin Nanozymes for Natural and Durable Targeted Treatment of Inflammatory Bowel Disease (IBD)

DOI: 10.1002/smll.202207350

Sample: colon tissue
Frontiers in Immunology (2021) IF: 7.561
Transcriptional Profiling and Functional Analysis of N1/N2 Neutrophils Reveal an Immunomodulatory Effect of S100A9-Blockade on the Pro-Inflammatory N1 Subpopulation

DOI: 10.3389/fimmu.2021.708770

Sample: neutrophils
Oxidative Medicine and Cellular Longevity (2022) IF: 6.543
MPO/HOCl Facilitates Apoptosis and Ferroptosis in the SOD1G93A Motor Neuron of Amyotrophic Lateral Sclerosis

DOI: 10.1155/2022/8217663

PMID: 35178161
Sample: plasma
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES (2022) IF: 5.112
Thermosensitive smart hydrogel of PEITC ameliorates the therapeutic efficacy in rheumatoid arthritis

DOI: 10.1016/j.ejps.2022.106367

Sample: serum
SAUDI JOURNAL OF BIOLOGICAL SCIENCES (2022) IF: 4.052
Evaluation of testicular torsion management in Ogbomoso, South-Western Nigeria and surgical detorsion-augmented treatment with phytochemical fractions of Corchorus olitorius leaf in experimental rats

DOI: 10.1016/j.sjbs.2022.103495

Sample: testicular tissue
ENVIRONMENTAL TOXICOLOGY (2022) IF: 4.109
hUC-MSCs exosomal miR-451 alleviated acute lung injury by modulating macrophage M2 polarization via regulating MIF-PI3K-AKT signaling pathway

DOI: 10.1002/tox.23639

Sample: serum,NR8383 cell
Foods (2022) IF: 4.35
Protective Effects of a Novel Probiotic Bifidobacterium pseudolongum on the Intestinal Barrier of Colitis Mice via Modulating the Pparγ/STAT3 Pathway and Intestinal Microbiota

DOI: 10.3390/foods11111551

Sample: colon tissue
JOURNAL OF ETHNOPHARMACOLOGY (2021) IF: 4.36
Revealing the mechanism of “Huai Hua San” in the treatment of ulcerative colitis based on network pharmacology and experimental study

DOI: 10.1016/j.jep.2021.114321

Sample: colon
Scientific Reports (2022) IF: 4.38
Promotion of the inflammatory response in mid colon of complement component 3 knockout mice

DOI: 10.1038/s41598-022-05708-8

PMID: 35105928
Sample: colon
INTERNATIONAL IMMUNOPHARMACOLOGY (2022) IF: 4.932
Gastroprotective effect of an active ingredients group of Lindera reflexa Hemsl. On Ethanol-Induced gastric ulcers in Rats: Involvement of VEGFR2/ERK and TLR-2/Myd88 signaling pathway

DOI: 10.1016/j.intimp.2022.108673

PMID: 35259712
Sample: stomach tissues
DISEASE MARKERS (2022) IF: 3.434
Knockdown of ILK Alleviates High Glucose-Induced Damage of H9C2 Cells through TLR4/MyD88/NF-κB Pathway

DOI: 10.1155/2022/6205190

Sample: H9C2 cell
JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2022) IF: 3.568
Examination of antimicrobial effect of fluoxetine in experimental sepsis model: An in vivo study

DOI: 10.1002/jbt.23240

Sample: blood
Immunity Inflammation and Disease (2021) IF: 2.239
Neutrophil extracellular traps induce thrombogenicity in severe carotid stenosis

DOI: 10.1002/iid3.466

PMID: 34102007
Sample: plasma
BMC Complementary and Alternative Medicine (2017) IF: 2.288
Extract from mango mistletoes Dendrophthoe pentandra ameliorates TNBS-induced colitis by regulating CD4+ T cells in mesenteric lymph nodes

DOI: 10.1186/s12906-017-1973-z

Sample: neutrophils
Journal of Experimental Zoology Part A-Ecological and Integrative Physiology (2022) IF: 2.693
Molecular mechanisms underlying ameliorative impact of melatonin against age-dependent chronic arsenic toxicity in rats' brains

DOI: 10.1002/jez.2603

Sample: brain

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Reviews/Q&A

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Q&A

M*****iSubmitted [ Oct 13 2022 ]

Asked: For tissue samples, can I calculate by protein content?

adminSubmitted [ Oct 13 2022 ]

Answered: NO, because the homogenate used in this kit has an effect on protein detection.

M*****iSubmitted [ Sep 06 2022 ]

Asked: Can I use PBS instead of reagent 1 as the homogenate?

adminSubmitted [ Sep 06 2022 ]

Answered: No. The homogenate can not be replaced by PBS, otherwise the sample will be extracted insufficiently, resulting in low results.

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