Ni Focurose 6FF (IDA)

    • Ni-4FF(IDA)-Elabscience
    • Ni-4FF(IDA)-Elabscience
    • Ni-4FF(IDA)-Elabscience
    <
    >
    • Ni-4FF(IDA)-Elabscience
    • Ni-4FF(IDA)-Elabscience
    • Ni-4FF(IDA)-Elabscience
    • Ni-4FF(IDA)-Elabscience
    • Ni-4FF(IDA)-Elabscience
    • Ni-4FF(IDA)-Elabscience

      Catalog number:E-CM-AF08

      Size:
      • 25mL
      • 100mL
      • 300mL
      • 500mL
      • 1L
      Qty:
      - +
      Price: $208

      Matrix: Highly cross-linked 4% agarose

      Particle size range: 45-165 µm

      Average particle size: 90 µm

      Pressure: ≤ 0.3 MPa

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Product introduction

      Ni-4FF (IDA) can be used for separation and purification with the interactions between Ni2+and some amino acids (mainly include histidine, cysteine, tryptophan) on the side chain of protein, and it is suitable for separation and purification of His-tagged protein and biological molecules which interact with Ni2+.

       

      Advantages

      1.  Quick and easy (one-step purification).

      2.  Wide application, simple-operation. Suitable for gravity column and prepacked column.

      3.  Multiple selection. Other metal ions (e.g. Cu2+, Zn2+, Fe2+, Co2+, Ca2+, etc.) can be used if Ni2+ cannot be applied well.

      Note: Avoid of using PB buffer when coupled with Ca2+ (precipitate will occur).

       

      Performance index

      Matrix

      Highly cross-linked 4% agarose

      Particle size range

      45-165 µm

      Average particle size

      90 µm

      Binding capacity

      40-50 mg (His-tagged protein)/mL (media)

      pH stability*

      3-12 (long-term)

      2-14 (short term)

      Chemical stability

      All of the commonly used solution. Avoid of chelating agent (e.g. EDTA, EGTA) and reductant (e.g. DTT, DTE).

      Flow rate

      300-600 cm/h

      (0.3MPa, XK16/40. Column bed height:30 cm)

      Pressure

      0.3 MPa

      Storage buffer

      20% Ethanol

      Storage temperature

      4~8


      *: The stability of media when unchelated with metal ions.


      Application examples

      Example 1




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