Ni Focurose 6FF(IMAC)
Price: $ 326
Price: $ 160
Size:
25mL
5mL
Quantity:
- Applications: Protein Purification and Separation
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Product Details
Introduction | Ni-6FF (IMAC) can be used for separation and purification with the interactions between Ni2+and some amino acids (mainly include histidine, cysteine, tryptophan) on the side chain of protein, and it is suitable for separation and purification of His-tagged protein and biological molecules which interact with Ni2+. |
Product Features |
1. Quick and easy (one-step purification). 2. Wide application, simple-operation. Suitable for gravity column and prepacked column. 3. Lower Ni2+ abscission probability and wider compatibility with reagents (Table 2) when compared with Ni-6FF (IDA). |
Matrix | Highly cross-linked 6% agarose |
Particle Size Range | 45-165 μm |
Average Particle Size | 90 μm |
Binding Capacity | 45 mg(His-tagged protein)/mL(media) |
Media Type | Chromatographic Media |
pH Stability | 3-12(long-term),2-14(short term) |
Chemical Stability | 0.01M HCl, 0.01M NaOH(1 week) 1MNaOH, 70% Ethanol(12 hours) 2% SDS(1 hour)30% Isopropyl alcohol(30 minutes) |
Maximum Flow Rate | 300-600 cm/h (0.3MPa, XK16/40. Column bed height:30 cm) |
Pressure | ≤0.3 MPa |
Storage Buffer | 20% Ethanol |
Storage | 4~8℃ |
Expiration Date | 36 months |
Shipping | Ice bag |