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Ni Focurose 6FF(IMAC)

Cat:E-CM-AF07
Manual MSDS

Price: $ 326

Price: $ 160

Size:
25mL 5mL
Quantity:
  • Applications: Protein Purification and Separation
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Product Details
Introduction Ni-6FF (IMAC) can be used for separation and purification with the interactions between Ni2+and some amino acids (mainly include histidine, cysteine, tryptophan) on the side chain of protein, and it is suitable for separation and purification of His-tagged protein and biological molecules which interact with Ni2+.
Product Features 1. Quick and easy (one-step purification).
2. Wide application, simple-operation. Suitable for gravity column and prepacked column.
3. Lower Ni2+ abscission probability and wider compatibility with reagents (Table 2) when compared with Ni-6FF (IDA).
Matrix Highly cross-linked 6% agarose
Particle Size Range 45-165 μm
Average Particle Size 90 μm
Binding Capacity 45 mg(His-tagged protein)/mL(media)
Media Type Chromatographic Media
pH Stability 3-12(long-term),2-14(short term)
Chemical Stability 0.01M HCl, 0.01M NaOH(1 week) 1MNaOH, 70% Ethanol(12 hours) 2% SDS(1 hour)30% Isopropyl alcohol(30 minutes)
Maximum Flow Rate 300-600 cm/h (0.3MPa, XK16/40. Column bed height:30 cm)
Pressure ≤0.3 MPa
Storage Buffer 20% Ethanol
Storage 4~8℃
Expiration Date 36 months
Shipping Ice bag