OQX (Olaquindox) ELISA Kit

    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
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    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience
    • Food Safety ELISA Kit-Elabscience

      Catalog number:E-FS-E007

      Size:
      • 96T
      Qty:
      - +
      Price: $443

      Sensitivity: 0.50 ppb

      Sample type: Tissue, Feed

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Test principle and application

      This kit uses Indirect-Competitive-ELISA as the method. It can detect OQX in samples, such as tissue, feed, etc. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody, standard and other supplementary reagents. The micro-plate provided in this kit has been pre-coated with OQX. During the reaction, OQX in the samples or standard competes with OQX on the solid phase supporter for sites of OQX antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of OQX. The concentration of OQX in the samples can be calculated by comparing the OD of the samples to the standard curve.

       

      Technical indicator

      Sensitivity: 0.5 ppb (ng/mL)

      Reaction mode: 37, 30 min30 min15 min

      Detection limit: Tissue---1.5 ppb    Feed---150 ppb

      Cross-reactivity: Olaquindox---100%,   Carbadox---0.1%

      Sample recovery rate: Tissue ---80%±15%    Feed---85%±15%

       

      Kits components


      Item

      Specifications

      ELISA Microtiter plate

      96 wells

      Standard Liquid

      1 mL each

      (0 ppb,0.5 ppb,1.5 ppb,4.5 ppb,13.5 ppb,40.5 ppb)

      High Concentrated Standard (1 ppm)

      1 mL

      HRP Conjugate 

      11 mL

      Antibody Working Solution

      5.5 mL

      Substrate Reagent A

      6 mL

      Substrate Reagent B

      6 mL

      Stop Solution

      6 mL

      20×Concentrated Wash Buffer 

      40 mL

      Reconstitution Buffer 

      50 mL

      Plate Sealer

      3 pieces

      Sealed Bag

      1 piece

      Manual

      1 copy


      Other supplies required

      Instruments: Microplate reader, Printer, Homogenizer, Nitrogen Evaporators/Water bath, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01 g).

      High-precision transferpettor: single channel (20-200 μL, 100-1000 μL), Multichannel (300 μL).

      Reagents: Anhydrous acetonitrile, methyl alcohol.


      Storage and valid period

      Storage: Store at 2-8. Avoid freeze / thaw cycles.

      Valid Period: 1 year, expiration  date is on the packing box.

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