OTA (Ochratoxin A) ELISA Kit

    • Mycotoxin Detection ELISA Kit-Elabscience
    • Mycotoxin Detection ELISA Kit-Elabscience
    <
    >
    • Mycotoxin Detection ELISA Kit-Elabscience
    • Mycotoxin Detection ELISA Kit-Elabscience
    • Mycotoxin Detection ELISA Kit-Elabscience
    • Mycotoxin Detection ELISA Kit-Elabscience

      Catalog number:E-TO-E001

      Size:
      • 96T
      Qty:
      - +
      Price: $280

      sample_type: Grain,Feed

      Sensitivity: 1 ppb

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Test principle

      This kit uses Competitive-ELISA as the method. It can detect Ochratoxin A (OTA) in samples, such as grain and feed. This kit is composed of ELISA Microtiter plate, HRP conjugate, antibody working solution, standard and other supplementary reagents. The microtiter plate provided in this kit has been pre-coated with coupled antigen. During the reaction, OTA in the samples or standard competes with coupled antigen on the solid phase supporter for sites of anti-OTA antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each microtiter plate well, and TMB substrate is added for color development. There is a negative correlation between the OD value of samples and the concentration of OTA. The concentration of OTA in the samples can be calculated by comparing the OD of the samples to the standard curve.



      Technical indicator

      Sensitivity: 1 ppb (ng/mL)

      Reaction mode: 37, 30 min30 min15 min

      Detection limit: Grain---5 ppb; Feed---10 ppb

      Cross-reactivity: Ochratoxin A ---100%

      Sample recovery rate:  85%±15%


      Kits components

      Item

      Specifications

      ELISA Microtiter plate


      96 wells

      Standard Liquid 

      1 mL each

      (0 ppb, 1 ppb, 3 ppb,9 ppb,27 ppb, 81 ppb)

      HRP Conjugate 

      11 mL

      Antibody Working Solution 

      5.5 mL

      Substrate Reagent A 

      6 mL

      Substrate Reagent B 

      6 mL

      Stop Solution 

      6 mL

      20×Concentrated Wash Buffer 

      40 mL

      Plate Sealer

      3 pieces

      Sealed Bag

      1 piece

      Manual

      1 copy


      Other supplies required

      Instrument: Microplate reader, Printer, Homogenizer, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01 g).

      High-precision transferpettor: Single channel (20-200 μL, 100-1000 μL), Multichannel (300 μL).

      Reagents: Methanol, NaHCO3.


      Storage and valid period

      Store at 2~8℃ for 1 year. Avoid freeze.

      Please store the opened kit at 2~8℃, the shelf life of the opend kit is up to 1 month.

      Expiry date: expiration date is on the packing box.


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