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(KO Validated) PDPK1 Polyclonal Antibody

Cat:E-AB-66885
Manual MSDS
  • +1

Price: $ 580

Price: $ 360

Price: $ 220

Size:
200μL 120μL 60μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human;Mouse;Rat
  • Applications: WB;IHC
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For research use only. Order now, ship in 3 days

Product Details
Verified Samples Verified Samples in WB:Mouse brain,Rat brain,HCT116
Verified Samples in IF:Rat brain
Dilution

WB 1:500-1:2000, IHC 1:50-1:200

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Recombinant fusion protein of human PDPK1
Abbre PDPK1
Synonyms PDPK1;PDK1;PDPK2;PDPK2P;PRO0461
Swissprot
Calculated MW 48kDa/50kDa/58kDa/60kDa/63kDa
Observed MW 58-68KDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Cell junction,Cell membrane,Cytoplasm,Nucleus,Peripheral membrane protein,focal adhesion
Concentration 1 mg/mL
Buffer PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Purification Method Affinity purification
Research Areas Cancer;Signal Transduction
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background PDPK1 (3-phosphoinositide dependent protein kinase 1) is a protein-coding gene. GO annotations related to this gene include protein serine/threonine kinase activity and insulin receptor binding.