Alternate Names	Integrin beta-2,Itgb2,Cell surface adhesion glycoproteins LFA-1/CR3/p150+95 subunit beta,Complement receptor C3 subunit beta,CD18
Swissprot	P11835
References	1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press. 2. Anderson DC, et al. 1987. Annu. Rev. Med. 38:175. 3. Wilson RW, et al. 1993. J. Immunol. 151:1571.

Product Details

Form	Liquid
Clone No.	M18/2(See Other Available Formats)
Host	Rat
Isotype	Rat IgG2a, κ
Isotype Control	PE Rat IgG2a, κ Isotype Control[2A3] [Product E-AB-F09832D]
Reactivity	Mouse
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	PE
	PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter).

How to select the appropriate detection channel through the spectrogram?

Background

CD18 is a 95 kD protein, also known as integrin β2 subunit. It is expressed on all leukocytes. CD18, in association with integrin α chain CD11a, CD11b, and CD11c forms LFA-1, Mac-1, and αXβ2, respectively, and plays an important role in leukocytes adhesion. The CD18 integrin complexes bind ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50), iC3b, and fibrinogen.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
AF/LE Purified Anti-Mouse CD18 Antibody[M18/2]	50μg, 500μg, 1mg	Check More
Purified Anti-Mouse CD18 Antibody[M18/2]	25μg, 100μg	Check More
Biotin Anti-Mouse CD18 Antibody[M18/2]	25μg, 100μg	Check More
FITC Anti-Mouse CD18 Antibody[M18/2]	50Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Mouse CD18 Antibody[M18/2]	50Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Mouse CD18 Antibody[M18/2]	50Tests, 100Tests, 100Tests×2	Check More
FITC Anti-Mouse CD18 Antibody[M18/2]	25μg, 100μg	Check More
PE Anti-Mouse CD18 Antibody[M18/2]	25μg, 100μg	Check More
PE/Cyanine7 Anti-Mouse CD18 Antibody[M18/2]	25μg, 100μg	Check More
Elab Fluor^® 488 Anti-Mouse CD18 Antibody[M18/2]	25μg, 100μg	Check More

Citations

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