PE/Cyanine5.5 Anti-Human CD28 Antibody[CD28.2] E-AB-F1195I

Uniprot : P10747
  • Host: Mouse
  • Applications: FCM
  • Isotype: Mouse IgG1, κ

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Antigen Details

Alternate Names PAS2,MNS,HGpMiX,GYPA,GPSAT
Swissprot P10747
References
1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. June CH, et al. 1994. Immunol. Today 15:321.
3. Linskey PS, et al. 1993. Annu. Rev. Immunol. 11:191.

Product Details

Form Liquid
Clone No. CD28.2(See Other Available Formats)
Host Mouse
Isotype Mouse IgG1, κ
Isotype Control PE/Cyanine5.5 Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09792I]
Reactivity Human
Application FCM (See the Application Description)
Storage Buffer PBS with 0.05% Proclin300, 1% BSA
Recommended Use Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The amount of the reagent is suggested to be used 5 μL of antibody per test (million cells in 100 μL staining volume or per 100 μL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8℃ and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation PE/Cyanine5.5

Background

CD28 is a 44 kD disulfide-linked homodimeric type I glycoprotein. It is a member of the immunoglobulin superfamily and is also known as T44 or Tp44. CD28 is expressed on most T lineage cells, NK cell subsets, and plasma cells. CD28 binds both CD80 and CD86 using a highly conserved motif MYPPY in the CDR3-like loop. CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death. In vitro studies indicate that ligation of CD28 on T cells by CD80 and CD86 on antigen presenting cells provides a costimulatory signal required for T cell activation and proliferation.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

Alternative Formats

Cat.No. Product Name Sizes
E-AB-F11950 AF/LE Purified Anti-Human CD28 Antibody[CD28.2] E-AB-F11950 50 μg, 500 μg, 1 mg
E-AB-F1195A Purified Anti-Human CD28 Antibody[CD28.2] E-AB-F1195A 25 μg, 100 μg
E-AB-F1195C FITC Anti-Human CD28 Antibody[CD28.2] E-AB-F1195C 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1195D PE Anti-Human CD28 Antibody[CD28.2] E-AB-F1195D 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1195E APC Anti-Human CD28 Antibody[CD28.2] E-AB-F1195E 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1195G PE/Cyanine5 Anti-Human CD28 Antibody[CD28.2] E-AB-F1195G 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1195H PE/Cyanine7 Anti-Human CD28 Antibody[CD28.2] E-AB-F1195H 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1195I PE/Cyanine5.5 Anti-Human CD28 Antibody[CD28.2] E-AB-F1195I 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1195J PerCP/Cyanine5.5 Anti-Human CD28 Antibody[CD28.2] E-AB-F1195J 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1195L AF488 Anti-Human CD28 Antibody[CD28.2] E-AB-F1195L 20 Tests, 50 Tests, 100 Tests, 200 Tests
E-AB-F1195M AF647 Anti-Human CD28 Antibody[CD28.2] E-AB-F1195M 20 Tests, 50 Tests, 100 Tests, 200 Tests

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