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Peroxidase (POD) Activity Assay Kit (Plant Samples)

Cat.No.:E-BC-K227-S
Detection instrument: Spectrophotometer(240 nm、420 nm)

Detection method: Colorimetric method

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Detection principle

The peroxidase can catalyze the decomposition of H2O2 and produce water and oxygen. And oxygen oxidized pyrogallic acid to form yellow product. The activity of peroxidase can be calculated by measuring the absorbance at 420 nm.

Performance characteristics

Synonyms	POD
Sample type	Plant tissue
Sensitivity	0.5 U/mL
Detection range	0.5-40 U/mL
Detection method	Colorimetric method
Assay type	Enzyme Activity
Assay time	60 min
Precision	Average inter-assay CV: 5.500%Average intra-assay CV: 3.800%
Other instruments required	Incubator, Vortex mixer, Micropipettor, Centrifuge
Other reagents required	Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage	2-8℃
Valid period	12 months

Images

Activity of POD was significantly induced in OsABCD43-OE9 group. (*P<0.05)

J Tian et al investigate the cadmium accumulation regulator and the harmful to host plant and leaf bacteria.. Peroxidase (POD) avtivity in crop leaf was determined using peroxidase activity assay kit (E-BC-K227-S).

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.5-40 U/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type	Dilution factor
10% Chinese rose tissue homogenization	1
10% Epipremnum aureum tissue homogenization	1
10% Green pepper tissue homogenization	2-3
10% Mushrooms tissue homogenization	1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Troubleshooting

Collection and treatment of common samples for metabolism assay detection

Questions and Answers of Metabolism Assay Kits

Citations

Journal of Advanced Research (2022) IF: 10.479
Cadmium accumulation regulated by a rice heavy-metal importer is harmful for host plant and leaf bacteria

DOI: 10.1016/j.jare.2022.05.010

PMID: 35640876
Sample: leaf
Communications Biology (2022) IF: 6.548
A recessive mutation in muscadine grapes causes berry color-loss without influencing anthocyanin pathway

DOI: 10.1038/s42003-022-04001-8

PMID: 36153380
Sample: skin
SAUDI JOURNAL OF BIOLOGICAL SCIENCES (2022) IF: 4.052
Physiological, biochemical and molecular evaluation of mungbean genotypes for agronomical yield under drought and salinity stresses in the presence of humic acid

DOI: 10.1016/j.sjbs.2022.103385

PMID: 35942166
Sample: leaf
EUROPEAN JOURNAL OF PHARMACOLOGY (2019) IF: 3.17
Pancreastatin inhibitor PSTi8 attenuates hyperinsulinemia induced obesity and inflammation mediated insulin resistance via MAPK/NOX3-JNK pathway

DOI: 10.1016/j.ejphar.2019.172723

PMID: 31586632
Sample: Plasma
Plants-Basel (2021) IF: 3.935
Salinity Effects on Morpho-Physiological and Yield Traits of Soybean (Glycine max L.) as Mediated by Foliar Spray with Brassinolide

DOI: 10.3390/plants10030541

Sample: leaf
HORTSCIENCE (2021) IF: 1.455
Effects of Salt Stress on Photosynthetic Fluorescence Characteristics, Antioxidant System, and Osmoregulation of Coreopsis tinctoria Nutt.

DOI: 10.21273/HORTSCI15956-21

Sample: seed

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Reviews/Q&A

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Reviews
Q&A

M*******aSubmitted [ Oct 24 2023 ]

Asked: I have a problem with using this kit. I want to determine POD activity in tomato leaves. Tissue extract is 50 mg in 0,45 ml PBS with protein content about 1.5-2 mg/ml. I dilute the extract 1, 5, 10 and 20-fold (100 mcl extract, 20 extract + 80 PBS, etc.) and take into account dilution factor (f in the formula) - 1, 5, 10 and 20, respectively. The problem is that OD420 values does not change properly - it declines from 0,31 to 0,25 in controls and from 0,7 to 0,57 in samples. Using the formula from manual I get increasing results of POD activity with declining extract concentration - 24,9 U/mgprot in sample without dilution and 430 U/mgprot in sample with dilution factor 20. Could you please give me some hint? Maybe I do something wrong in my calculations?

adminSubmitted [ Oct 24 2023 ]

Answered: After-sales questions will be answered by email

M**********dSubmitted [ Aug 25 2022 ]

Asked: Would this kit work for rose petals? It may produce some reddish purple extract, I found the detection wavelehgth is 420 nm, will it interfered?

adminSubmitted [ Aug 25 2022 ]

Answered: The kit is suitable for all plant samples even we didn't verify that whether the color would be a background, you may take a pre-test to see if it needs a control test, or we can do this verification for you if you'd like to contact with our technical support.

D********dSubmitted [ Aug 03 2022 ]

Asked: Can the kit work for animal tissue samples?

adminSubmitted [ Aug 03 2022 ]

Answered: This kit works for plant tissue only.

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