Pg(Progesterone) ELISA Kit

    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    <
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    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience
    • competitive-ELISA-Elabscience

      Catalog number:E-EL-0090

      Size:
      • 96T
      • 24T
      Qty:
      - +
      Price: $495

      Reactivity: Universal

      Detection Range: 0.50~30 ng/mL

      Sensitivity: 0.20 ng/mL

      Lead Time: 7~10 daysWelcome to order from local distributors.

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      可替代产品推荐: Pg(Progesterone) ELISA Kit

      Intended use

      This ELISA kit applies to the in vitro quantitative determination of Pg concentrations in serum, plasma and other biological fluids.


      Test principle

      This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with Goat Anti-Rabbit IgG, make solid-phase secondary antibody. And then add samples, horseradish peroxidase-labeled Progesterone and anti- Progesterone antibody, so as to form a coated secondary antibody - anti- Progesterone antibody - HRP-labeled Progesterone complex. The amount of bound labeled Progesterone is inversely proportional to that of Progesterone in the samples. The TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Progesterone in the samples is then determined by comparing the OD of the samples to the standard curve.


      Assay type

      Competitive

      Format

      96T

      Assay time

      2h

      Reactivity

      Universal

      Detection Method

      Colormetric

      Detection Range

      0.5—30 ng/mL

      Sensitivity

      0.2 ng/mL

      Sample Volume

      50μL

      Sample Type

      Serum, plasma and other biological fluids


      Specificity

      No significant cross-reactivity or interference with pregnenolone, androstenedione, estradiol.     


      Kit components & Storage

      An unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received


      Item

      Specifications

      Storage

      Micro ELISA Plate(Dismountable)  

      8 wells ×12 strips

      4

      Reference Standard (6 tubes)

      0.5mL/tube#

      4

      Detection Ab

      1vial  6mL

      4

      HRP-labeled Progesterone

      1vial  6mL

      4℃(shading light)

      Concentrated Wash Buffer (20×)

      1vial  15mL

      4

      Substrate Reagent A

      1vial  7mL

      4(shading light)

      Substrate Reagent B

      1vial  7mL

      4(shading light)

      Stop Solution

      1vial  7mL

      4

      Plate Sealer

      5pieces

       

      Product Description

      1 copy

       

      Certificate of Analysis

      1 copy

       


      #: A set of Standard concentrations is 0 ng/ mL, 0.5 ng/ mL, 2 ng/ mL, 6 ng/ mL, 15 ng/ mL, 30ng/ mL.

      The volume of reagents in partial shipments is a little more than the volume marked on the label, please use in measuring instead of directly pouring.


      Other supplies required

      Microplate reader with 450nm wavelength filter

      High-precision transferpettor, EP tubes and disposable pipette tips

      37 Incubator

      Deionized or distilled water

      Absorbent paper

      Loading slot for Wash Buffer


      Citations

      1. Publication: Yang X, Zhang W, Chen Y, et al. Activation Of Pparγ And Cd36 Expression--The Dual Pathophysiological Roles Of Progesterone[J]. Journal of Biological Chemistry, 2016.
        Sample Type: Serum
      2. Publication: Zheng Q, Li Y L, Zhang D D, et al. ANP Promotes Proliferation And Inhibits Apoptosis Of Ovarian Granulosa Cells By NPRA/PGRMC1/EGFR Complex And Improves Ovary Functions Of PCOS Rats[J]. Cell Death & Disease, 2017.
        Sample Type: Serum
      3. Publication: Kong L H, Wang Q E, Jin J W, et al. Insulin Resistance Enhances The Mitogen-Activated Protein Kinase Signaling Pathway In Ovarian Granulosa Cells[J]. Plos One, 2017.
        Sample Type: culture supernatant
      4. Publication: Yang C, Ye J, Liu Y, et al. Methylation pattern variation between goats and rats during the onset of puberty[J]. Reproduction in Domestic Animals, 2018.
      5. Publication: Wang M, Li L, Liu R, et al. Obesity-induced overexpression of miRNA-24 regulates cholesterol uptake and lipid metabolism by targeting SR-B1[J]. Gene, 2018, 668: 196-203.
        Sample Type: Mouse
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