Phenyl-6HP can interact with some hydrophobic protein or antibody under high ionic strength conditions (high ionic strength may increase the interaction between ligand and hydrophobic groups), thus to achieve the purpose of separation and purification. Phenyl-6HP is mainly used for fine purification of samples.

Product Features

1. Rapid, easy to use (one-step purification).
2. Compared with reversed-phase chromatography, the ligand concentration in the hydrophobic interaction chromatography media is low and the elution conditions are mild, which helps to maintain the biological activity of biomolecules.
3. Wide application. Phenyl-6BB can be applied in fine purification, and it can be repeatedly used in combination ion exchange chromatography media.
4. High resolution.

Product Datasheet

Matrix	Highly cross-linked 6% agarose
Particle size range	25-45 µm
Average particle size	34 µm
Binding capacity	30 mg(IgG)/mL(media)
Ligand	Phenyl
Ligand density	25-30 µmol/mL
Media type	Hydrophobic Interaction Medium
pH stability	2-14(short-term) 3-13(long-term)
Chemical stability	All of the commonly used buffers, 8M urea, 6M guanidine hydrochloride
Maximum flow rate	150 cm/h
Storage buffer	20% Ethanol
Storage temperature	4~30℃(4~8℃ is preferred)

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Cat. No	E-CM-HI03
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