Phospho-Chk1/2 Antibody Sampler Kit

    • Phospho-Chk1/2 Antibody Sampler Kit -Elabscience
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    • Phospho-Chk1/2 Antibody Sampler Kit -Elabscience
    • Phospho-Chk1/2 Antibody Sampler Kit -Elabscience

      Catalog number:E-AB-K3004

      Size:
      • 9*20μL
      Qty:
      - +
      Price: $497

      Applications: WB

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      Product Includes

      Product name Specifications Application
      CHEK1 Polyclonal Antibody 20μL WB,IHC-p,IF,ELISA
      Phospho-CHEK1 (Ser280) Polyclonal Antibody 20μL WB,ELISA
      Phospho-CHEK1 (Ser317) Polyclonal Antibody 20μL WB,IHC-p,ELISA
      Phospho-CHEK1 (Ser301) Polyclonal Antibody 20μL WB,IF,ELISA
      CHEK2 Polyclonal Antibody 20μL WB,IHC-p,ELISA
      Phospho-CHEK2 (Ser516) Polyclonal Antibody 20μL WB,ELISA
      Phospho-CHEK2 (Thr68) Polyclonal Antibody 20μL WB,IHC-p,IF,ELISA
      Phospho-CHEK2 (Thr383) Polyclonal Antibody 20μL WB,IF,ELISA
      Phospho-CHEK2 (Thr387) Polyclonal Antibody 20μL WB,IHC-p,ELISA
      Goat Anti-Rabbit IgG (H+L)(peroxidase/HRP conjugated) 120μL WB,IHC,ELISA

      Product Photos

      Western Blot analysis of K562 cells with CHEK1 Polyclonal Antibody

      Western Blot analysis of 293T cells using Phospho-CHEK1 (Ser280) Polyclonal Antibody at dilution of 1:500

      Western Blot analysis of 293T cells using Phospho-CHEK1 (Ser317) Polyclonal Antibody at dilution of 1:500

      Western Blot analysis of 293T cells using Phospho-CHEK1 (Ser301) Polyclonal Antibody at dilution of 1:2000

      Western Blot analysis of 22RV1 cells using CHEK2 Polyclonal Antibody at dilution of 1:2000.

      Western Blot analysis of COS7 cells with Phospho-CHEK2 (Ser516) Polyclonal Antibody

      Western Blot analysis of Hela cells using Phospho-CHEK2 (Thr68) Polyclonal Antibody at dilution of 1:500

      Western Blot analysis of Hela cells using Phospho-CHEK2 (Thr383) Polyclonal Antibody at dilution of 1:1000

      Western Blot analysis of Hela cells using Phospho-CHEK2 (Thr387) Polyclonal Antibody at dilution of 1:500

      Product description

      The Phospho-Chk1/2 Antibody Sampler Kit offers an economical means to evaluate the phosphorylation status of Chk1 and Chk2 on multiple residues. The kit contains enough primary and secondary antibodies to perform two Western blot experiments with each primary antibody.

      Background

      Chk1 kinase acts downstream of ATM/ATR kinase and plays an important role in DNA damage checkpoint control, embryonic development, and tumor suppression. Activation of Chk1 involves phosphorylation at Ser317 and Ser345 by ATM/ATR, followed by autophosphorylation of Ser296. Activation occurs in response to blocked DNA replication and certain forms of genotoxic stress. While phosphorylation at Ser345 serves to localize Chk1 to the nucleus following checkpoint activation, phosphorylation at Ser317 along with site-specific phosphorylation of PTEN allows for re-entry into the cell cycle following stalled DNA replication. Chk1 exerts its checkpoint mechanism on the cell cycle, in part, by regulating the cdc25 family of phosphatases. Chk1 phosphorylation of cdc25A targets it for proteolysis and inhibits its activity through 14-3-3 binding. Activated Chk1 can inactivate cdc25C via phosphorylation at Ser216, blocking the activation of cdc2 and transition into mitosis. Centrosomal Chk1 has been shown to phosphorylate cdc25B and inhibit its activation of CDK1-cyclin B1, thereby abrogating mitotic spindle formation and chromatin condensation. Furthermore, Chk1 plays a role in spindle checkpoint function through regulation of aurora B and BubR1. Research studies have implicated Chk1 as a drug target for cancer therapy as its inhibition leads to cell death in many cancer cell lines. Chk2 is the mammalian homologue of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases. The amino-terminal domain of Chk2 contains a series of seven serine or threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50 and Thr68) followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases. Indeed, after DNA damage by ionizing radiation (IR), UV irradiation and DNA replication blocked by hydroxyurea, Thr68 and other sites in this region become phosphorylated by ATM/ATR. The SQ/TQ cluster domain, therefore, seems to have a regulatory function. Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 on residues Thr383 and Thr387 in the activation loop of the kinase domain.

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