PNPO Polyclonal Antibody
- +1
Price: $ 399
Price: $ 240
Price: $ 143
Price: $ 73
- Host: Rabbit
- Reactivity: Human; Mouse; Rat
- Applications: WB;IHC
For research use only. Order now, ship in 3 days
Verified Samples |
Verified Samples in WB:293T Verified Samples in IHC:Human brain,Human gastric cancer |
Dilution |
WB 1:500-1:2000, IHC 1:20-1:100 Western Blot Operation Guide |
Clonality | Polyclonal |
Immunogen | Synthetic peptide of human PNPO |
Abbre | PNPO |
Synonyms | EC 1.4.3.5;FLJ10535;PDXPO;PNPO;PNPO;Pyridoxal 5' phosphate synthase;pyridoxamine 5' phosphate oxidase;Pyridoxamine phosphate oxidase ;Pyridoxamine-phosphate oxidase;pyridoxine 5' phosphate oxidase;Pyridoxine-5'-phosphate oxidase |
Swissprot | |
Calculated MW | 30 kDa |
Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Concentration | 0.9 mg/mL |
Buffer | PBS with 0.05% NaN3 and 40% Glycerol,pH7.4 |
Purification Method | Antigen affinity purification |
Research Areas | Metabolism; Neuroscience; Signal transduction |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | Ice bag |
background | The enzyme encoded by this gene catalyzes the terminal, rate-limiting step in the synthesis of pyridoxal 5'-phosphate, also known as vitamin B6. Vitamin B6 is a required co-factor for enzymes involved in both homocysteine metabolism and synthesis of neurotransmitters such as catecholamine. Mutations in this gene result in pyridoxamine 5'-phosphate oxidase (PNPO) deficiency, a form of neonatal epileptic encephalopathy. |