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PNPO Polyclonal Antibody

Cat:E-AB-19759
Manual MSDS
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Price: $ 399

Price: $ 240

Price: $ 143

Price: $ 73

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human; Mouse; Rat
  • Applications: WB;IHC
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Product Details
Verified Samples Verified Samples in WB:293T
Verified Samples in IHC:Human brain,Human gastric cancer
Dilution

WB 1:500-1:2000, IHC 1:20-1:100

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Synthetic peptide of human PNPO
Abbre PNPO
Synonyms EC 1.4.3.5;FLJ10535;PDXPO;PNPO;PNPO;Pyridoxal 5' phosphate synthase;pyridoxamine 5' phosphate oxidase;Pyridoxamine phosphate oxidase ;Pyridoxamine-phosphate oxidase;pyridoxine 5' phosphate oxidase;Pyridoxine-5'-phosphate oxidase
Swissprot
Calculated MW 30 kDa
Observed MW Refer to figures
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Concentration 0.9 mg/mL
Buffer PBS with 0.05% NaN3 and 40% Glycerol,pH7.4
Purification Method Antigen affinity purification
Research Areas Metabolism; Neuroscience; Signal transduction
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background The enzyme encoded by this gene catalyzes the terminal, rate-limiting step in the synthesis of pyridoxal 5'-phosphate, also known as vitamin B6. Vitamin B6 is a required co-factor for enzymes involved in both homocysteine metabolism and synthesis of neurotransmitters such as catecholamine. Mutations in this gene result in pyridoxamine 5'-phosphate oxidase (PNPO) deficiency, a form of neonatal epileptic encephalopathy.