Porcine Circovirus Type 2 Antibodies ELISA Kit

    • Animal Diseases ELISA Kit-Elabscience
    • Animal Diseases ELISA Kit-Elabscience
    <
    >
    • Animal Diseases ELISA Kit-Elabscience
    • Animal Diseases ELISA Kit-Elabscience
    • Animal Diseases ELISA Kit-Elabscience
    • Animal Diseases ELISA Kit-Elabscience

      Catalog number:E-AD-E003

      Size:
      • 96T
      Qty:
      - +
      Price: $203

      Reactivity: Swine

      Sample type: Serum, Plasma

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Test principle

      This kit is comprised by ELISA Microtiter plate pre-coated with recombinant cap protein of porcine circovirus (PCV2), HRP conjugate and other auxiliary reagents and apply the principle of enzyme-linked immunoassay (ELISA) to detect porcine circovirus antibody of porcine serum, plasma samples. During the experiment, add control serum and samples into the ELISA Microtiter plate. If PCV2 antibodies exist in the samples, it will be bound with the antigen pre-coated on the Microtiter plate after incubation. Then wash the plate to remove unbound antibodies and other components, add the HRP Conjugate to specifically bind with the compound of antibody and antigen on the Microtiter plate. The unbound HRP Conjugate will be removed by washing. TMB is added into the well, it will react with the enzyme and become blue. The color shade is of positive correlation with antibody levels in the samples. At last, end the reaction by adding stop solution to produce a yellow product. Measure the absorbance value of each well by using a Microtiter plate Reader with 450 nm wavelength, then the presence of PCV2 antibody can be determined.

       

      Kit components

      Item

      Specification

      ELISA Microtiter plate

      96 wells

      Dilution plate
      96 wells

      HRP Conjugate 

      11 mL

      Sample Diluent 

      50 mL

      20×Concentrated Wash Buffer

      40 mL

      Substrate Reagent

      6 mL

      Substrate Reagent B

      6 mL

      Stop Solution 

      6 mL

      Positive Control 

      1 mL

      Negative Control 

      1 mL

      Plate Sealer

      pieces

      Sealed Bag

      piece

      Manual

      1 copy

       

      Experimental instrument

      Microplate Reader with 450nm wavelength filter or dual-wavelength (450/630nm)

      High-precision transferpettor, EP tubes and disposable pipette tips

      37incubator or water bath

      Deionized or distilled water

      Absorbent paper



      Storage and Expiry date

      Store at 2~8℃. Avoid freeze for 12 months.

      Please store the opened plate at 2~8℃, protect from light and moisture. The valid period is 1 months.

      Expiry date: expiration date is on the packing box.

      • Show all (0)
      • Reviews (0)
      • Q&A (0)
      ... Show All Show Less
      MSDS for ELISA

      Browsing History


        People Also Bought