Porcine Foot and Mouth Disease Virus NSP Antibodies ELISA Kit

    • Animal Diseases ELISA Kit-Elabscience
    • Animal Diseases ELISA Kit-Elabscience
    <
    >
    • Animal Diseases ELISA Kit-Elabscience
    • Animal Diseases ELISA Kit-Elabscience
    • Animal Diseases ELISA Kit-Elabscience
    • Animal Diseases ELISA Kit-Elabscience

      Catalog number:E-AD-E043

      Size:
      • 96T
      Qty:
      - +
      Price: $305

      Reactivity: Swine

      Sample type: Serum

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual


      Test principle

      This ELISA kit is only used as the qualitative detection of Foot and Mouth Disease Virus (FMDV) non-structural protein (NSP) Antibody in porcine. The ELISA Microtiter plate provided in this kit has been pre-coated with FMDV-NSP antigen, Control solution and samples are added into Micro-plate and incubate. If FMD-NSP antibody exist in the samples, it will be bound with the antigen pre-coated on the micro-plate. Then wash the plate to remove unbound antibodies and other components, add the HRP conjugate to specifically bind with the compound of antibody and antigen on the microplate. The unbound HRP conjugate will be removed by washing. Add TMB substrate in the wells, the shade of color is of negative correlation with FMDV-NSP antibody levels in the samples. Finally, end the reaction by adding stop solution. Measure the absorbance value of each well by using a Microplate reader with 450 nm wavelength, then the presence of FMDV-NSP antibody can be determined.

       

      Kit components

      Item

      Specification

      ELISA Microtiter plate

      96 wells

      HRP Conjugate

      11 mL

      Sample Diluent

      100 mL

      10×Concentrated Wash Buffer

      100 mL

      Chromogenic agent

      11 mL

      Stop Solution

      15 mL

      Negative Control

      2 mL

      Positive Control

      1 mL

      Plate Sealer

      3 pieces

      Sealed Bag

      1 piece

      Manual

      1 copy

       

      Experimental instrument

      Microplate Reader with 450 nm wavelength filter or dual-wavelength (450/630 nm)

      High-precision transferpettor: Single channel (10-100 μL, 100-1000 μL)

      Measuring Cylinder: 500mL, EP tubes and disposable pipette tips

      Deionized or distilled water

      Absorbent paper

      Storage and Expiry date

      Store at 2~8with shading light for 12 months.

      Please store the opened plate at 2~8, protect from light and moisture. The valid period is 2 months.

      Expiry date: expiration date is on the packing box.


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