Porcine Japanese Encephalitis Virus Antibodies ELISA Kit

    • Animal Diseases ELISA Kit-Elabscience
    • Animal Diseases ELISA Kit-Elabscience
    <
    >
    • Animal Diseases ELISA Kit-Elabscience
    • Animal Diseases ELISA Kit-Elabscience
    • Animal Diseases ELISA Kit-Elabscience
    • Animal Diseases ELISA Kit-Elabscience

      Catalog number:E-AD-E002

      Size:
      • 96T
      Qty:
      - +
      Price: $203

      Reactivity: Swine

      Sample type: Serum, Plasma

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Test principle

      This kit is comprised by ELISA Microtiter plate pre-coated with recombinant Porcine Japanese Encephalitis Virus (JEV) E2 protein, HRP Conjugate and other auxiliary reagents, and apply the principle of enzyme-linked immunoassay (ELISA) to detect porcine JEV antibody of porcine serum, plasma samples. During the experiment, add control serum and samples into the ELISA Microtiter plate. If JEV specific antibodies exist in the samples after incubation, it will be bound with the recombinant protein on the micro-plate. Then wash the plate to remove unbound antibodies and other components, add the HRP Conjugate to specifically bind with the compound of antibody and antigen on the Micro-plate. The unbound HRP Conjugate will be removed by washing. TMB is added into the well, it will react with the enzyme and the products become blue. The color shade is of positive correlation with specific antibody levels in the samples. At last, end the reaction by adding Stop Solution to produce a yellow product. Measure the absorbance value of each well by using a Micro-plate Reader with 450 nm wavelength, then we can know whether there are porcine PJEV antibody in the samples.

       

      Kit components

      Item

      Specification

      ELISA Microtiter plate

      96 wells

      HRP Conjugate

      11 mL

      Sample Diluent 

      50 mL

      20×Concentrated Wash Buffer

      40 mL

      Substrate Reagent

      6 mL

      Substrate Reagent B

      6 mL

      Stop Solution

      6 mL
      Positive Control 

      1 mL

      Negative Control 

      1 mL

      Plate Sealer

      3 pieces

      Sealed Bag

      1 piece
      Manual
      1 copy

       

      Experimental instrument

      Microplate Reader with 450 nm wavelength filter or dual-wavelength (450/630 nm)

      High-precision transferpettor, EP tubes and disposable pipette tips

      37 incubator or water bath

      Deionized or distilled water

      Absorbent paper

       

      Storage and Expiry date

      Store at 2~8with shading light for 12 months.

      Please store the opened plate at 2~8, protect from light and moisture. The valid period is 2 months.

      Valid Period: expiration date is on the packing box.



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