Porcine Transmissible Gastroenteritis Virus Antibodies ELISA Kit

    • Animal Diseases ELISA Kit-Elabscience
    • Animal Diseases ELISA Kit-Elabscience
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    >
    • Animal Diseases ELISA Kit-Elabscience
    • Animal Diseases ELISA Kit-Elabscience
    • Animal Diseases ELISA Kit-Elabscience
    • Animal Diseases ELISA Kit-Elabscience

      Catalog number:E-AD-E041

      Size:
      • 96T
      Qty:
      - +
      Price: $360

      Reactivity: Swine

      Sample type: Serum,plasma

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual


      Test principle

      This kit applies the ELISA method for the in vitro qualitative detection of concentration of antibody to Porcine Transmissible Gastroenteritis Virus (TGEV) in samples. The ELISA Microtiter plate provided in this kit has been pre-coated with the TGEV antigen. In experiment, add control serum and samples into plate. If TGEV antibodies exist in the samples, it will be bound with the antigen pre-coated on the micro-plate. Then wash to remove unbound antibodies and other components, add the HRP Conjugate to specifically bind with the compound of antibody and antigen on the Micro-plate; the unbound HRP Conjugate will be removed by washing. Add TMB substrate in the wells, it will react with the enzyme and become blue, the shade of color is of positive correlation with TGEV antibody levels in the samples. At last, end the reaction by adding Stop Solution to produce a yellow product. Measure the absorbance value of each well by using a Micro-plate Reader with 450 nm wavelength, then we can judge whether TGEV antibody exist in the sample.

       

      Kit components

      Item

      Specifications

      ELISA Microtiter plate

       96 wells

      Dilution plate
       96 wells

      HRP Conjugate 

      11 mL

      Sample Diluent 

      50 mL

      20×Concentrated Wash Buffer

      40 mL

      Substrate Reagent

      6 mL

      Substrate Reagent B

      6 mL

      Stop Solution 

      6 mL

      Positive Control 

      1 mL

      Negative Control 

      1 mL

      Plate Sealer

      3 pieces

      Sealed Bag
      1 piece

      Manual

      1 copy

       

      Experimental instrument

      Microplate Reader with 450nm wavelength filter or dual-wavelength (450/630nm)

      High-precision transferpettor, EP tubes and disposable pipette tips

      37 incubator or water bath

      Deionized or distilled water

      Absorbent paper


      Assay procedure
      1. Number: Take out the Micro-plate, set 1 well for blank control and 2 wells for negative/positive control respectively. The unused ELISA Microtiter plate should be sealed as soon as possible and stored at 2~8℃. Double well parallel experiment is recommended for detection.
      2. Add sample: Add 100 µL of sample diluent to the blank control well, add 100 μL of positive/negative control to positive/negative control well. Add 100 µL of diluted sample to the sample wells. 
      3. Incubate: Gently tap the plate to mix thoroughly and incubate at 37℃ for 30 min.
      4. Wash: remove the liquid in each well. Immediately add 300 μL of wash buffer to each well and wash. Repeat wash procedure for 5 times, 30 s intervals/time. Invert the plate and pat it against thick clean absorbent paper (If bubbles exist in the wells, clean tips can be used to prick them).
      5. HRP conjugate: Add 100 µL of HRP conjugate into each well (except the blank control well) and incubate at 37℃ for 30 min. 
      6. Wash: Repeat Step 4 for washing.
      7. Color Development: Add 50 µL of Substrate Reagent A and 50 µL of Substrate Reagent B into each well, gently tap the plate to mix thoroughly. Incubate at 37℃ for 10 min with shading light.
      8. Stop reaction: Add 50 µL of Stop Solution into each well and mix thoroughly. 
      9. OD Measurement: Determine the absorbance (A-value) of each well at 450 nm with a micro-plate reader (the 450/630 nm double wavelength is recommended). This step should be finished in 10 min after stop reaction.

      Storage and Expiry date

      Store at 2~8with shading light for 12 months.

      Please store the opened plate at 2~8, protect from light and moisture. The valid period is 2 months.

      Valid Period: expiration date is on the packing box.


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