Potassium (K) Colorimetric Assay Kit (With standard)

    • Biochemical Assay Kit-Elabscience
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    >
    • Biochemical Assay Kit-Elabscience
    • Biochemical Assay Kit-Elabscience

      Catalog number:E-BC-K279

      Size:
      • 96T
      Qty:
      - +
      Price: $150

      Detection method: Colorimetric method

      Detection instrument: Microplate reader

      Valid period: 6 months

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      General information

      Detection significance

      Potassium ions are vital for the functioning of all living cells. The transfer of potassium ions across nerve cell membranes is necessary for normal nerve transmission; potassium deficiency and excess can each result in numerous signs and symptoms, including an abnormal heart rhythm and various electrocardiographic abnormalities. Fresh fruits and vegetables are good dietary sources of potassium. The body responds to the influx of dietary potassium, which raises serum potassium levels, with a shift of potassium from outside to inside cells and an increase in potassium excretion by the kidneys.

      Detection principle

      Under the alkaline condition, the sodium tetraphenylborate reacts with the potassium ions in the sample to form the potassium tetraphenylborate which is white and small particles with small solubility. Potassium tetraphenylborate particles are in a stable suspension state in the solution. The turbidity is proportional to the potassium ion concentration in the sample and potassium content can be calculated indirectly by measuring the OD value at 450 nm.

      The key point

      1. Prevent the formulation of bubbles when adding the liquid to the microplate.

      2. Hemolysis samples should not be adopted since red blood cells contain high concentrations of potassium ions.

      3. Ammonia, mercury, chlorine can interfere with the determination of potassium ion.

      4. It is recommended to use deionized water to prepare tissue homogenate and avoid potassium ion pollution.

      5. It is suggested to measure the total protein content when detecting the potassium content in tissue or cells (E-BC-K165, E-BC-K168 and E-BC-K318).

      Operation procedures

      Plate set up

       

      1

      2

      3

      4

      5

      6

      7

      8

      9

      10

      11

      12

      A

      A

      A

      S1

      S9

      S17

      S25

      S33

      S41

      S49

      S57

      S65

      S73

      B

      B

      B

      S2

      S10

      S18

      S26

      S34

      S42

      S50

      S58

      S66

      S74

      C

      C

      C

      S3

      S11

      S19

      S27

      S35

      S43

      S51

      S59

      S67

      S75

      D

      D

      D

      S4

      S12

      S20

      S28

      S36

      S44

      S52

      S60

      S68

      S76

      E

      E

      E

      S5

      S13

      S21

      S29

      S37

      S45

      S53

      S61

      S69

      S77

      F

      F

      F

      S6'

      S14

      S22

      S30

      S38

      S46

      S54

      S62

      S70

      S78

      G

      G

      G

      S7

      S15

      S23

      S31

      S39

      S47

      S55

      S63

      S71

      S79

      H

      H

      H

      S8

      S16

      S24

      S32

      S40

      S48

      S56

      S64

      S72

      S80

                                                    [Note]: A-H, standard wells; S1-S80, sample wells.

      The dilution of standard curve

      Dilute 1 mmol/L Potassium standard solution with deionized water to a serial concentration. The recommended dilution gradient is as follows: 0.8, 0.6, 0.5, 0.4, 0.3, 0.2, 0.1, 0 μmol/L.

      Operation steps

      1)   Standard tube: Take 50 μL of Standard solution with different concentrations to the wells.

      Sample tube: Take 50 μL of supernatant (pretreated Sample) to the wells.

      2)   Add 200 μL of Chromogenic agent into the wells of Step 1.

      3)   Mix fully and stand for 5 min at room temperature.

      4)   Measure the OD value at 450 nm with Microplate Reader.

      Operation table

       

      Standard well

      Sample well

      Potassium standard solution with different concentrations (μL)

      50

       

      Sample supernatant (μL)

       

      50

      Chromogenic agent (μL)

      200

      200

      Mix fully and stand for 5 min. Measure the OD value at 450 nm.

      Performance characteristics

      Technical parameter

      Detection range 0.01-0.80 mmol/L Average inter-assay CV 6.1%
      Sensitivity 0.002 mmol/L Average intra-assay CV 1.1%
      Average recovery rate 94%

      Example analysis

      For Rat liver tissue, take 0.1 g of fresh rat liver sample, add 0.9 mL of 2-8deionized water, then homogenize treat the sample in ice water bath, centrifuge at 10000 g for 10 min at 4℃, then dilute the supernatant with deionized water for 2 times and carry the assay according to the operation table.

      The results are as follows:

      standard curve: y = 0.77073 x – 0.00139, the average OD value of the sample well is 0.404, the average OD value of the blank well is 0.045, the concentration of protein in sample is 9.23 gprot/L, and the calculation result is:

      K+content (mmol/gprot)=0.404-0.045+0.00139÷0.77073×10×2÷9.23=1.01mmol/gprot

      Detect rat serum, 10% rat liver tissue homogenate (the concentration of protein is 9.23 gprot/L dilute for 2 times), culture supernatant of RAW264.7 cells and RAW264.7 cells (the concentration of protein is 4.40 gprot/L) according to the protocol, the result is as follows:
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