Procaspase Antibody Sampler Kit

    • Procaspase Antibody Sampler Kit -Elabscience
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    • Procaspase Antibody Sampler Kit -Elabscience
    • Procaspase Antibody Sampler Kit -Elabscience

      Catalog number:E-AB-K3110

      Size:
      • 7*20μL
      Qty:
      - +
      Price: $386

      Applications: WB

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      Product Includes

      Product name Specifications Application
      CASP3 Polyclonal Antibody 20μL WB,IHC-p,IF,ELISA
      CASP6 Polyclonal Antibody 20μL WB,IHC-p,IF,ELISA
      CASP7 Polyclonal Antibody 20μL WB,IHC-p,ELISA
      CASP8 Polyclonal Antibody 20μL WB,IHC-p,IF,ELISA
      CASP9 Polyclonal Antibody 20μL WB,IHC-p,IF,ELISA
      LMNA Polyclonal Antibody 20μL WB,IHC-p,IF,ELISA
      PARP1 Polyclonal Antibody 20μL WB,ELISA
      Goat Anti-Rabbit IgG (H+L)(peroxidase/HRP conjugated) 120μL WB,IHC,ELISA

      Product Photos

      Western Blot analysis of KB, MCF7 cells using CASP3 Polyclonal Antibody at dilution of 1:1000.

      Western Blot analysis of HepG2 cells using CASP6 Polyclonal Antibody at dilution of 1:1000.

      Western Blot analysis of HT29 cells using CASP7 Polyclonal Antibody at dilution of 1:1000.

      Western Blot analysis of 3T3 cells with CASP8 Polyclonal Antibody

      Western Blot analysis of various cells using CASP9 Polyclonal Antibody at dilution of 1:500.

      Western Blot analysis of 3T3 cells using LMNA Polyclonal Antibody at dilution of 1:2000.

      Western Blot analysis of Rat musle using PARP1 Polyclonal Antibody at dilution of 1:1000.

      Product description

      The Procaspase Antibody Sampler Kit provides an economical means to evaluate the abundance and activation of caspases. The kit contains enough primary antibody to perform at least two western blots per primary antibody.

      Background

      Apoptosis is a regulated physiological process leading to cell death. Caspases, a family of cysteine acid proteases, are central regulators of apoptosis. Initiator caspases (including 2, 8, 9, 10 and 12) are closely coupled to proapoptotic signals, which include the FasL, TNF-α, and DNA damage. Once activated, these caspases cleave and activate downstream effector caspases (including 3, 6 and 7), which in turn cleave cytoskeletal and nuclear proteins like PARP, α-fodrin, DFF and lamin A, and induce apoptosis. Caspase-8 (FLICE, Mch5, MACH) and Caspase-9 (ICE-LAP6, Mch6) are initiator caspases. CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activate caspase-8, leading to the release of the caspase-8 active fragments, p18 and p10. Cytochrome c released from the mitochondria associates with procaspase-9 (47 kDa)/Apaf 1. Apaf-1 mediated activation of caspase-9 involves intrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Another cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response. Caspase-3 (CPP-32, Apoptain, Yama, SCA-1), Caspase-6 (Mch2), and Caspase-7 (CMH-1, Mch3, ICE-LAP3) are effector caspases. Activation of caspase-3 requires proteolytic processing of its inactive zymogen/proform into activated p17 and p12 subunits. Procaspase-7 is activated through proteolytic processing by upstream caspases at Asp23, Asp198, and Asp206 to produce the mature subunits. Procaspase-6 is cleaved by caspase-3 at Asp23, Asp179 and Asp193 to form active large (p18) and small (p11) subunits. PARP, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress . This protein can be cleaved by many ICE-like caspases in vitro and is one of the main cleavage targets of caspase-3 in vivo. In human PARP, the cleavage occurs between Asp214 and Gly215, which separates the PARP amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa). PARP helps cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis. Lamins are nuclear membrane structural components that are important in maintaining normal cell functions such as cell cycle control, DNA replication, and chromatin organization. Lamin A/C is cleaved by caspase-6 and serves as a marker for caspase-6 activation. During apoptosis, lamin A/C is specifically cleaved into large (41-50 kDa) and small (28 kDa) fragments. The cleavage of lamins results in nuclear disregulation and cell death.

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