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PUM3 Polyclonal Antibody

Cat:E-AB-18185
Manual MSDS

Price: $ 399

Price: $ 240

Price: $ 143

Price: $ 73

Size:
200μL 120μL 60μL 20μL
Quantity:
  • Host: Rabbit
  • Reactivity: Human
  • Applications: WB
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Product Details
Verified Samples Verified Samples in WB:Jurkat,Raji
Dilution

WB 1:1000-1:5000

Western Blot Operation Guide
Clonality Polyclonal
Immunogen Synthetic peptide of human PUM3
Abbre PUM3
Synonyms HA-8;HBV X transactivated gene 5 protein;HBV X-transactivated gene 5 protein;HBV XAg-transactivated protein 5;HLA HA8;HLA-HA8;hPUF-A;K0020;Kiaa0020;KIAA0020 protein;MGC8749;Minor histocompatibility antigen HA 8;Minor histocompatibility antigen HA-8;PEN;Penguin homolog;Protein 5 transactivated by hepatitis B virus X antigen (HBxAg);Protein 5 transactivated by hepatitis B virus X antigen;PUF 6;PUF6;Pumilio domain containing protein KIAA0020;Pumilio domain-containing protein KIAA0020;RP11 526D20.2;XTP 5;XTP5
Swissprot
Calculated MW 74 kDa
Observed MW Refer to figures
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Cellular Localization Nucleus>nucleolus.
Concentration 1.32 mg/mL
Buffer PBS with 0.05% NaN3 and 40% Glycerol,pH7.4
Purification Method Antigen affinity purification
Research Areas Cell Biology
Conjugation Unconjugated
Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping Ice bag
background Inhibits the poly(ADP-ribosyl)ation activity of PARP1 and the degradation of PARP1 by CASP3 following genotoxic stress. Binds to double-stranded RNA or DNA without sequence specificity. Involved in development of the eye and of primordial germ cells.