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QuicKey Human CLU(Clusterin) ELISA Kit

Uniprot : P10909
  • Cat.No.:E-TSEL-H0014

  • Reactivity: Human

To Purchase E-TSEL-H0014

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $390
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Product Details

QuicKey Series

Get more sensitive and precise results with saving at least 1h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 2.5h
Detection range 1.56-100 ng/mL
Sensitivity 0.94 ng/mL
Sample type &Sample volume serum, plasma, urine, saliva; 50μL
Specificity This kit recognizes Human CLU in samples. No significant or interference between Human CLU and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human CLU concentrations in serum, plasma, urine, saliva.Please consult technical support for the applicability if other biological fluids need to be tested.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CLU. Samples (or Standards) and biotinylated detection antibody specific for Human CLU are added to the micro ELISA plate wells. Human CLU would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CLU, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human CLU. You can calculate the concentration of Human CLU in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for six months. After test, the unused wells and reagents should be stored according to the table below.
ItemSpecificationsStorage conditions after test
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
2-8℃, 1 month
Reference Standard 96T: 2 vials
48T: 1 vial
Discard unused reconstituted standard and dilutions
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8℃
Biotinylated Detection Ab Working Solution 1 vial, 6 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
2-8℃ (Protect from light)
Substrate Reagent 1 vial, 10 mL
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Product Description 1 copy
Certificate of Analysis 1 copy

Technical Data

Sample Values

sandwich-Ab-ELISA-Elabscience

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human CLU were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human CLU were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 4.57 10.92 48.94 4.60 12.45 41.92
Standard deviation 0.24 0.51 1.99 0.24 0.62 1.95
CV (%) 5.25 4.67 4.07 5.22 4.98 4.65

Recovery

The recovery of Human CLU spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-101 97
EDTA plasma(n=8) 88-98 95
Urine(n=8) 83-96 91

Linearity

Samples were spiked with high concentrations of Human CLU and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

sandwich-Ab-ELISA-Elabscience

Target Information

Background

Clusterin is a ubiquitous protein that is arousing increasing interest owing to its widespread diffusion and multifunctional role. It is composed of two 40 kD subments (NA1, NA2) encoded by a single gene, which hold together by disulphide bonds. The mature dimer has a molecular mass of 80 kD and is micro-heterogeneous in electrical charge due to glycosylation. This protein has been differently named as apolipoprotein J (ApoJ), sulfated glycoprotein 2 (SGP-2), glycoprotein III (GP- III), testosterone repressed prostate message (TRPM-2), SP-40, 40cytolysis inhibitor (CL1) and others [1].It is a component of the high-density lipoprotein (HDL) complex with putative functions in the transport of lipids, apoptosis, protection of cells from stress and, most important, in the regulation of complement activity. It has been postulated that clusterin is involved in a variety of human diseases including Alzheimer disease, brain injury following ischemia,nephrosis and myocarditis [2].
1. Wilson M R, Easterbrooksmith S B. Clusterin is a secreted mammalian chaperone. [J]. Trends in Biochemical Sciences, 2000, 25(3):95-98.
2. Mclaughlin L, Zhu G, Mistry M, et al. Apolipoprotein J/clusterin limits the severity of murine autoimmune myocarditis. [J]. Journal of Clinical Investigation, 2000, 106(9):1105-1113.
Database LinksSwissProt: P10909
Entrez Gene: 1191
SynonymsApo-J, APOJ, KUB1, CLI, NA1/NA2, SGP-2, TRPM-2, Aging-associated gene 4 protein, Apolipoprotein J, Complement cytolysis inhibitor, Complement-associated protein SP-40,40, Ku70-binding protein 1, Sulfated glycoprotein 2, Testosterone-repressed prostate message 2
Research AreaImmunology,Cardiovascular,Metabolism,Signaling transduction

Assay Procedures

step01

1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 90 min at 37°C

step02

2. Aspirate and wash the plate for 3 times

step03

3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

step04

4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

step05

5. Add 50μL Stop Solution

step06

6. Read the plate at 450nm immediately. Calculation of the results

Citations

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