QuicKey Human CRP(C-Reactive Protein) ELISA Kit

Uniprot : P02741
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    <
    >
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience
    • sandwich-Ab-ELISA-Elabscience

      Catalog number:E-TSEL-H0007

      Synonyms:PTX1

      Size:
      • 96T
      • 48T
      Qty:
      - +
      Price: $390

      Reactivity: Human

      Detection Range: 0.39~25 ng/mL

      Sensitivity: 0.23 ng/mL

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request

      Product Details

      QuicKey Series

      Get more sensitive and precise results with saving at least 1h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

      Properties

      Assay type Sandwich-ELISA
      Format 96T/48T
      Assay time 2.5 h
      Reactivity Human
      Detection method Colormetric
      Colormetric 0.39-25 ng/mL
      Sensitivity 0.23 ng/mL
      Sample volume 50μL/well
      Sample type serum, plasma
      Specificity This kit recognizes Human CRP in samples. No significant cross-reactivity or interference between Human CRP and analogues was observed.
      Reproducibility Both intra-CV and inter-CV are < 10%.
      Application This ELISA kit applies to the in vitro quantitative determination of Human CRP concentrations in serum, plasma. Please consult technical support for the applicability if other biological fluids need to be tested.

      Test principle

      This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CRP. Samples (or Standards) and biotinylated detection antibody specific for Human CRP are added to the micro ELISA plate wells. Human CRP would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human CRP. You can calculate the concentration of Human CRP in the samples by comparing the OD of the samples to the standard curve.

      Kit components & Storage

      An unopened kit can be stored at 2-8℃ for six months. After test, the unused wells and reagents should be stored according to the table below. (The specific components might be slightly different in delivered kits, please refer to the manual for more information.)

      Item Specifications Storage conditions after test
      Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips
      48T: 8 wells ×6 strips
      2-8℃, 1 month
      Reference Standard 96T: 2 vials
      48T: 1 vial
      Discard unused reconstituted standard and dilutions
      Reference Standard & Sample Diluent 1 vial, 20 mL 2-8℃
      Biotinylated Detection Ab Working Solution 1 vial, 6 mL
      HRP Conjugate Diluent 1 vial, 14 mL
      Concentrated Wash Buffer (25×) 1 vial, 30 mL
      Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
      48T: 1 vial, 60 μL
      2-8℃ (Protect from light)
      Substrate Reagent 1 vial, 10 mL
      Stop Solution 1 vial, 10 mL 2-8℃
      Plate Sealer 5 pieces
      Product Description 1 copy
      Certificate of Analysis 1 copy

      Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution. The volume of reagents in partial shipments is a little more than the volume marked on the label, please use accurate measuring equipment instead of directly pouring into the vial(s).

      Other supplies required

      1. Microplate reader with 450nm wavelength filter

      2. High-precision transfer pipette, EP tubes and disposable pipette tips

      3. Incubator capable of maintaining 37℃

      4. Deionized or distilled water

      5. Absorbent paper

      6. Loading slot for Wash Buffer

      Technical Data

      Sample values

      sandwich-Ab-ELISA-Elabscience

      Typical Standard Curve

      As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.

      Concentration(ng/mL) 25 12.5 6.25 3.13 1.56 0.78 0.39 0
      OD 2.434 1.587 1.027 0.607 0.353 0.211 0.134 0.068
      Corrected OD 2.366 1.519 0.959 0.539 0.285 0.143 0.066 -
      sandwich-Ab-ELISA-Elabscience

      Precision

      Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human CRP were tested 20 times on one plate, respectively.

      Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human CRP were tested on 3 different plates, 20 replicates in each plate.

      Intra-assay Precision Inter-assay Precision
      Sample 1 2 3 1 2 3
      n 20 20 20 20 20 20
      Mean(ng/mL) 1.19 3.11 11.37 1.18 2.73 11.41
      Standard deviation 0.07 0.15 0.55 0.06 0.13 0.51
      CV (%) 5.88 4.82 4.84 5.08 4.76 4.47

      Recovery

      The recovery of Human CRP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

      Sample Type Range (%) Average Recovery (%)
      Serum(n=8) 95-103 98
      EDTA plasma(n=8) 95-108 103

      Linearity

      Samples were spiked with high concentrations of Human CRP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

      Serum(n=4) EDTA plasma (n=4)
      1:2 Range (%) 91-103 94-103
      Average (%) 96 98
      1:4 Range (%) 95-105 98-109
      Average (%) 99 102
      1:8 Range (%) 91-104 89-96
      Average (%) 99 92
      1:16 Range (%) 95-105 83-96
      Average (%) 99 91

      Target information

      Background

      C-reactive protein (CRP) is a member of the phylogenetically ancient and highly conserved ‘pentraxin’ family of proteins, which also includes serum amyloid P component (SAP), a constituent of all amyloid deposits [1]. In man and other animal species, CRP is a major acute-phase plasma protein displaying rapid and pronounced rise of its serum concentration in response to infection or tissue injury.CRP is used mainly as a marker of inflammation. Apart from liver failure, there are few known factors that interfere with CRP production. Interferon alpha inhibits CRP production from liver cells which may explain the relatively low levels of CRP found during viral infections compared to bacterial infections [2]. Measuring and charting CRP values can prove useful in determining disease progress or the effectiveness of treatments. ELISA, immunoturbidimetry, nephelometry, rapid immunodiffusion, and visual agglutination are all methods used to measure CRP.
      1. Gewurz, Henry. Biology of C-Reactive Protein and the Acute Phase Response [J]. Hospital Practice, 1982, 17(6):67-81.
      2. Enocsson H , Christopher Sjöwall, Skogh T , et al. Interferon-alpha mediates suppression of C-reactive protein: explanation for muted C-reactive protein response in lupus flares? [J]. Arthritis & Rheumatism, 2010, 60(12):3755-3760.

      Research area

      Database Links

      SwissProt P02741 Human
      Entrez Gene 1401 Human

      Synonyms

      PTX1

      Documents

      Citations

      Please kindly contact us if you have published a paper by citing any products from Elabscience, we will get back to you with the Elabscience Publication Reward ASAP.

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      Assay procedures

      step01 1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 90 min at 37°C
      step02 2. Aspirate and wash the plate for 3 times
      step03 3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
      step04 4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
      step05 5. Add 50μL Stop Solution
      step06 6. Read the plate at 450nm immediately. Calculation of the results
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      MSDS for ELISA ISO 9001

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